Antagonistic and protective activity of Lactobacillus plantarum strain 17 M against E. amylovora.
2,3-Butadione
Acetic acid
Biocontrol
E. amylovora
Fire blight
Lactobacillus plantarum
Journal
World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472
Informations de publication
Date de publication:
21 Sep 2023
21 Sep 2023
Historique:
received:
20
02
2023
accepted:
14
09
2023
medline:
22
9
2023
pubmed:
21
9
2023
entrez:
21
9
2023
Statut:
epublish
Résumé
The subject of the study was to identify the antagonism of the Lactobacillus plantarum strain 17 M against the causative agent of fire blight, the bacterium Erwinia amylovora, and to evaluate its protective capabilities on apple blossoms against this disease. For comparison 9 strains of lactic acid bacteria from the LLP "SPC of Microbiology and Virology" collection were included in the study. Strain 17 M appeared to be superior in limiting the growth of the pathogen on all 3 liquid media tested. Its maximum inhibitory activity was proved on MRS Broth medium. The analysis of the secondary metabolites produced by strain 17 M in liquid medium revealed that it consisted mainly of acetic acid (53.2 ± 4.3%), lactic acid (16.3 ± 2.3%) and 2,3-butanedione (14.84 ± 4.1%). The presence of other organic compounds was also detected but in a smaller amount. Study on influence of those compounds on growth of E. amylovora showed that lactic acid at concentration of 5% showed inhibitory activity but it was not toxic to apple flowers. The effectiveness of strain 17 M culture liquid diluted with sterile water and added to E. amylovora inoculum at 10% or 20% was 76.7 ± 5.8% and 88.3 ± 12.6%, respectively. This study confirms the potential use of strain 17 M as an active microbial agent to combat fire blight of fruit crops in Kazakhstan.
Identifiants
pubmed: 37733156
doi: 10.1007/s11274-023-03765-3
pii: 10.1007/s11274-023-03765-3
doi:
Substances chimiques
Acetic Acid
Q40Q9N063P
Culture Media
0
Lactic Acid
33X04XA5AT
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
314Subventions
Organisme : Ministry of Science and Higher Education of the Republic of Kazakhstan
ID : BR18574022
Organisme : Ministry of Science and Higher Education of the Republic of Kazakhstan
ID : BR18574022
Organisme : Ministry of Science and Higher Education of the Republic of Kazakhstan
ID : BR18574022
Organisme : Ministry of Science and Higher Education of the Republic of Kazakhstan
ID : BR18574022
Organisme : Ministry of Science and Higher Education of the Republic of Kazakhstan
ID : BR18574022
Organisme : Ministry of Science and Higher Education of the Republic of Kazakhstan
ID : BR18574022
Organisme : Ministry of Science and Higher Education of the Republic of Kazakhstan
ID : BR18574022
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature B.V.
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