A CYP78As-small grain4-coat protein complex Ⅱ pathway promotes grain size in rice.
Journal
The Plant cell
ISSN: 1532-298X
Titre abrégé: Plant Cell
Pays: England
ID NLM: 9208688
Informations de publication
Date de publication:
30 Nov 2023
30 Nov 2023
Historique:
received:
11
07
2023
accepted:
11
08
2023
medline:
4
12
2023
pubmed:
22
9
2023
entrez:
22
9
2023
Statut:
ppublish
Résumé
CYP78A, a cytochrome P450 subfamily that includes rice (Oryza sativa L.) BIG GRAIN2 (BG2, CYP78A13) and Arabidopsis thaliana KLUH (KLU, CYP78A5), generate an unknown mobile growth signal (referred to as a CYP78A-derived signal) that increases grain (seed) size. However, the mechanism by which the CYP78A pathway increases grain size remains elusive. Here, we characterized a rice small grain mutant, small grain4 (smg4), with smaller grains than its wild type due to restricted cell expansion and cell proliferation in spikelet hulls. SMG4 encodes a multidrug and toxic compound extrusion (MATE) transporter. Loss of function of SMG4 causes smaller grains while overexpressing SMG4 results in larger grains. SMG4 is mainly localized to endoplasmic reticulum (ER) exit sites (ERESs) and partially localized to the ER and Golgi. Biochemically, SMG4 interacts with coat protein complex Ⅱ (COPⅡ) components (Sar1, Sec23, and Sec24) and CYP78As (BG2, GRAIN LENGTH 3.2 [GL3.2], and BG2-LIKE 1 [BG2L1]). Genetically, SMG4 acts, at least in part, in a common pathway with Sar1 and CYP78As to regulate grain size. In summary, our findings reveal a CYP78As-SMG4-COPⅡ regulatory pathway for grain size in rice, thus providing new insights into the molecular and genetic regulatory mechanism of grain size.
Identifiants
pubmed: 37738653
pii: 7280383
doi: 10.1093/plcell/koad239
pmc: PMC10689148
doi:
Substances chimiques
Plant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4325-4346Subventions
Organisme : National Key R&D Program of China
ID : 2021YFF1000200
Organisme : National Natural Science Foundation of China
ID : 31830064
Organisme : International Science & Technology Innovation Program of Chinese Academy of Agricultural Sciences
Organisme : Central Public-Interest Scientific Institution Basal Research Found, China
ID : Y2021YJ18
Organisme : Frontier exploration project of Jiangsu Province
ID : BK20212010
Organisme : Key project for Jiangsu Agricultural New Variety Innovation
ID : PZCZ201701
Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.
Déclaration de conflit d'intérêts
Conflict of interest statement. The authors declare no conflicts of interests.
Références
CSH Protoc. 2008 Apr 01;2008:pdb.prot4995
pubmed: 21356813
Front Plant Sci. 2020 May 27;11:691
pubmed: 32536934
Plant Physiol. 2020 Jul;183(3):974-985
pubmed: 32327549
Curr Biol. 2010 Mar 23;20(6):527-32
pubmed: 20188559
Plant Cell Environ. 2015 Apr;38(4):800-11
pubmed: 25255828
Dev Cell. 2007 Dec;13(6):843-56
pubmed: 18061566
J Exp Bot. 2019 Sep 24;70(18):4643-4656
pubmed: 31106838
Mol Plant. 2018 May 7;11(5):736-749
pubmed: 29567449
Plant Methods. 2011 Sep 30;7(1):30
pubmed: 21961694
Plant Physiol. 2021 May 27;186(1):767-781
pubmed: 33620479
Plant Mol Biol. 2023 Feb;111(3):291-307
pubmed: 36469200
Proc Natl Acad Sci U S A. 2020 May 5;117(18):9884-9895
pubmed: 32321832
Annu Rev Biochem. 2010;79:777-802
pubmed: 20533886
Nat Genet. 2011 Oct 23;43(12):1266-9
pubmed: 22019783
Plant Cell. 2007 Jun;19(6):2023-38
pubmed: 17601828
Nat Plants. 2017 Apr 10;3:17043
pubmed: 28394310
Plant J. 2007 Sep;51(6):1126-36
pubmed: 17666025
Plant Physiol. 2019 May;180(1):381-391
pubmed: 30796160
Proc Natl Acad Sci U S A. 2009 Nov 24;106(47):20115-20
pubmed: 19892740
Annu Rev Cell Dev Biol. 2016 Oct 6;32:197-222
pubmed: 27298089
Nature. 2006 Jan 12;439(7073):234-8
pubmed: 16407955
Mol Biol Rep. 2015 Feb;42(2):489-96
pubmed: 25324172
Plant Physiol. 2006 Nov;142(3):945-62
pubmed: 16980567
Nature. 1993 Sep 23;365(6444):347-9
pubmed: 8377826
Plant Cell. 2016 Feb;28(2):583-96
pubmed: 26744218
Sci Adv. 2021 Jan 15;7(3):
pubmed: 33523901
Plant J. 2012 Jun;70(6):929-39
pubmed: 22251317
BMC Plant Biol. 2016 Sep 26;16(1):207
pubmed: 27669820
Plant Cell. 2007 Jan;19(1):296-319
pubmed: 17209124
Nat Genet. 2007 May;39(5):623-30
pubmed: 17417637
Nat Commun. 2017 Mar 16;8:14752
pubmed: 28300078
EMBO J. 2002 Nov 15;21(22):6105-13
pubmed: 12426382
Plant J. 2015 Jul;83(2):312-25
pubmed: 26043144
Nat Commun. 2018 Feb 27;9(1):852
pubmed: 29487282
J Biol Chem. 2002 Feb 15;277(7):5360-8
pubmed: 11739388
Nat Commun. 2017 Nov 21;8(1):1633
pubmed: 29158478
Plant J. 2011 Mar;65(6):882-96
pubmed: 21251105
Plant J. 1994 Aug;6(2):271-82
pubmed: 7920717
Plant Cell. 2016 Nov;28(11):2850-2865
pubmed: 27803308
Plant Cell. 2014 Jul;26(7):3132-47
pubmed: 25082856
Plant Cell. 2000 Sep;12(9):1541-50
pubmed: 11006330
Plant Cell. 2015 Aug;27(8):2288-300
pubmed: 26276834
Plant Cell. 2004 Mar;16(3):672-93
pubmed: 14973159
Plant Mol Biol. 2016 Jan;90(1-2):33-47
pubmed: 26482479
Trends Plant Sci. 2016 Sep;21(9):758-769
pubmed: 27317568
Mol Plant. 2021 Feb 1;14(2):330-343
pubmed: 33246053
Plant J. 2015 Aug;83(3):427-38
pubmed: 26043067
Nat Commun. 2018 Mar 27;9(1):1240
pubmed: 29588443
Proc Natl Acad Sci U S A. 2013 Oct 15;110(42):17125-30
pubmed: 24082112
Nat Genet. 2008 Aug;40(8):1023-8
pubmed: 18604208
Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):11102-7
pubmed: 26283354
Proc Natl Acad Sci U S A. 2015 Nov 17;112(46):14360-5
pubmed: 26578783
Plant Physiol. 2009 Jan;149(1):297-305
pubmed: 19011004
Nat Genet. 2015 Aug;47(8):949-54
pubmed: 26147620
J Exp Bot. 2018 Mar 24;69(7):1615-1633
pubmed: 29390074
Curr Opin Plant Biol. 2016 Oct;33:23-32
pubmed: 27294659
Plant Physiol. 2021 Mar 15;185(2):352-368
pubmed: 33721894
J Exp Bot. 2013 Jul;64(10):2831-45
pubmed: 23682119
Front Plant Sci. 2017 Dec 05;8:2076
pubmed: 29259616
Plant Mol Biol. 2020 Nov;104(4-5):429-450
pubmed: 32808190
PLoS Genet. 2017 Jul 14;13(7):e1006906
pubmed: 28708858
Plant J. 1999 Aug;19(3):259-68
pubmed: 10476073
Mol Plant. 2014 Oct;7(10):1522-32
pubmed: 24851876
Annu Rev Plant Biol. 2010;61:421-42
pubmed: 20192739
Annu Rev Plant Biol. 2019 Apr 29;70:435-463
pubmed: 30795704
Nature. 2002 Sep 19;419(6904):271-7
pubmed: 12239560
Theor Appl Genet. 2006 Apr;112(6):1164-71
pubmed: 16453132
Science. 2001 Nov 16;294(5546):1528-31
pubmed: 11711675
PLoS Genet. 2014 Jan;10(1):e1003954
pubmed: 24391508
Plant Cell. 2020 Mar;32(3):758-777
pubmed: 31949008
Proc Natl Acad Sci U S A. 2021 Apr 27;118(17):
pubmed: 33879613
Biochim Biophys Acta. 2013 Nov;1833(11):2464-72
pubmed: 23419775
Mol Plant. 2013 Nov;6(6):1945-60
pubmed: 23775595
Plant Cell. 2014 Mar;26(3):1053-68
pubmed: 24619610
Plant Mol Biol. 2008 Jun;67(3):283-94
pubmed: 18322804