Biotransformation Of l-Tryptophan To Produce Arcyriaflavin A With Pseudomonas putida KT2440.
P. putida KT2440
arcyriaflavin A production
biotransformation
design of experiment
strain engineering
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
01 Dec 2023
01 Dec 2023
Historique:
revised:
20
09
2023
received:
16
08
2023
medline:
4
12
2023
pubmed:
25
9
2023
entrez:
24
9
2023
Statut:
ppublish
Résumé
Natural products such as indolocarbazoles are a valuable source of highly bioactive compounds with numerous potential applications in the pharmaceutical industry. Arcyriaflavin A, isolated from marine invertebrates and slime molds, is one representative of this group and acts as a cyclin D1-cyclin-dependent kinase 4 inhibitor. To date, access to this compound has mostly relied on multi-step total synthesis. In this study, biosynthetic access to arcyriaflavin A was explored using recombinant Pseudomonas putida KT2440 based on a previously generated producer strain. We used a Design of Experiment approach to analyze four key parameters, which led to the optimization of the bioprocess. By engineering the formation of outer membrane vesicles and using an adsorbent in the culture broth, we succeeded to increase the yield of arcyriaflavin A in the cell-free supernatant, resulting in a nearly eight-fold increase in the overall production titers. Finally, we managed to scale up the bioprocess leading to a final yield of 4.7 mg arcyriaflavin A product isolated from 1 L of bacterial culture. Thus, this study showcases an integrative approach to improve biotransformation and moreover also provides starting points for further optimization of indolocarbazole production in P. putida.
Identifiants
pubmed: 37743253
doi: 10.1002/cbic.202300576
doi:
Substances chimiques
arcyriaflavin A
0
Tryptophan
8DUH1N11BX
Carbazoles
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202300576Subventions
Organisme : German Federal Ministry of Education and Research
Organisme : NO-STRESS project
ID : 031B0852B
Organisme : Bioeconomy Science Center
Organisme : NRW Strategieprojekt
Organisme : Ministry of Culture and Science of the German federal state of North Rhine-Westphalia
ID : 313/323-400-00213
Informations de copyright
© 2023 The Authors. ChemBioChem published by Wiley-VCH GmbH.
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