Genomic Analysis of Antifungal Drug Resistance Induced in Trichophyton rubrum After Prolonged Culture with Terbinafine.
Genome analysis
Mutation
Resistance induction
Terbinafine
Trichophyton rubrum
Journal
Mycopathologia
ISSN: 1573-0832
Titre abrégé: Mycopathologia
Pays: Netherlands
ID NLM: 7505689
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
18
08
2023
accepted:
05
09
2023
medline:
1
12
2023
pubmed:
29
9
2023
entrez:
29
9
2023
Statut:
ppublish
Résumé
In this study, we induced terbinafine (TRF) resistance in a T. rubrum strain in vitro for 18 months then compared the genomes of the TRF-resistant (N42-3) and TRF-susceptible wild-type (N42WT) strains to identify mutations. In the SQLE gene, N42WT had no mutation while N42-3 had a F397L mutation. We sequenced approximately 22.53 Mb of the genomes of the N43WT and N42-3 strains. Other than the F397L mutation in SQLE, there were three other genetic mutations in three different genes that were found in N42-3, but not in N43WT; however, these three mutations were not detected in other TRF-resistant T. rubrum strains. From this genome sequencing analysis, the only variation that was confirmed to be associated with drug resistance in the genome of the TRF-resistant T. rubrum was a hotspot mutation in SQLE.
Identifiants
pubmed: 37773561
doi: 10.1007/s11046-023-00795-1
pii: 10.1007/s11046-023-00795-1
doi:
Substances chimiques
Terbinafine
G7RIW8S0XP
Antifungal Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1079-1083Subventions
Organisme : Japan Society for the Promotion of Science
ID : 22K08442
Organisme : Research Organization (ACRO) Incubation Grant from Teikyo
ID : In23-87
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature B.V.
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