Synthetic Gene Circuits Combining CRISPR Interference and CRISPR Activation in
CRISPR activation
CRISPR interference
bacterial synthetic biology
dCas9
resource competition
synthetic gene circuits
Journal
ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075
Informations de publication
Date de publication:
20 10 2023
20 10 2023
Historique:
medline:
23
10
2023
pubmed:
10
10
2023
entrez:
9
10
2023
Statut:
ppublish
Résumé
Gene expression control based on clustered regularly interspaced short palindromic repeats (CRISPR) has emerged as a powerful approach for constructing synthetic gene circuits. While the use of CRISPR interference (CRISPRi) is already well-established in prokaryotic circuits, CRISPR activation (CRISPRa) is less mature, and a combination of the two in the same circuits is only just emerging. Here, we report that combining CRISPRi with SoxS-based CRISPRa in
Identifiants
pubmed: 37813387
doi: 10.1021/acssynbio.3c00375
pmc: PMC10594877
doi:
Substances chimiques
RNA
63231-63-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3064-3071Références
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