UV-based solvent system screening for high-speed counter-current chromatography fractionation of compounds with similar UV absorption from complex samples followed by preparative HPLC purification: Flavonoids from barley seedlings as sample.
counter-current chromatography
flavonoids
fractionation
preparative HPLC
ultraviolet spectrophotometry screening
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
revised:
09
09
2023
received:
02
08
2023
accepted:
28
09
2023
medline:
11
12
2023
pubmed:
11
10
2023
entrez:
11
10
2023
Statut:
ppublish
Résumé
This article proposes a solvent system screening strategy for compounds with similar UV absorption in complex samples by UV spectrophotometer. There is no need to calculate the partition coefficient value of each compound, only the partition coefficient of the whole sample. The partition coefficient value should be close to 1 in order to obtain as many high-speed counter-current chromatography fractions as possible. Then, preparative HPLC was used to purify the high-speed counter-current chromatography fractions. Based on the above strategy, seven c-glycosyl flavonoids and an amino acid were successfully obtained from barley seedlings through high-speed counter-current chromatography fractionation with ethyl acetate/n-butanol/water (8:2:10, v:v:v) system followed by preparative HPLC purification. The research shows that high-speed counter-current chromatography could be well developed as a tool for fractionation before purification, and greatly improves the separation efficiency.
Identifiants
pubmed: 37817348
doi: 10.1002/jssc.202300558
doi:
Substances chimiques
Flavonoids
0
Solvents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300558Subventions
Organisme : Basic Research Program of Qinghai Province
ID : 2022-ZJ-721
Organisme : National Natural Science Foundation of China
ID : 82174052
Informations de copyright
© 2023 Wiley-VCH GmbH.
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