Genome-wide chromatin interaction map for Trypanosoma cruzi.
Journal
Nature microbiology
ISSN: 2058-5276
Titre abrégé: Nat Microbiol
Pays: England
ID NLM: 101674869
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
14
03
2023
accepted:
25
08
2023
medline:
8
11
2023
pubmed:
13
10
2023
entrez:
12
10
2023
Statut:
ppublish
Résumé
Trypanosomes are eukaryotic, unicellular parasites, such as Trypanosoma brucei, which causes sleeping sickness, and Trypanosoma cruzi, which causes Chagas disease. Genomes of these parasites comprise core regions and species-specific disruptive regions that encode multigene families of surface glycoproteins. Few transcriptional regulators have been identified in these parasites, and the role of spatial organization of the genome in gene expression is unclear. Here we mapped genome-wide chromatin interactions in T. cruzi using chromosome conformation capture (Hi-C), and we show that the core and disruptive regions form three-dimensional chromatin compartments named C and D. These chromatin compartments differ in levels of DNA methylation, nucleosome positioning and chromatin interactions, affecting genome expression dynamics. Our data reveal that the trypanosome genome is organized into chromatin-folding domains and transcription is affected by the local chromatin structure. We propose a model in which epigenetic mechanisms affect gene expression in trypanosomes.
Identifiants
pubmed: 37828247
doi: 10.1038/s41564-023-01483-y
pii: 10.1038/s41564-023-01483-y
pmc: PMC10627812
doi:
Substances chimiques
Chromatin
0
Membrane Glycoproteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2103-2114Subventions
Organisme : Agencia Nacional de Investigación e Innovación (National Agency for Research and Innovation)
ID : DCI-ALA/2011/023-502
Organisme : Agencia Nacional de Investigación e Innovación (National Agency for Research and Innovation)
ID : POS_NAC_2016_1_129916
Organisme : Agencia Nacional de Investigación e Innovación (National Agency for Research and Innovation)
ID : FCE_3_2022_1_172653
Organisme : Universidad de la República Uruguay (University of the Republic Uruguay)
ID : BFPD_2021_1#45569540
Organisme : Universidad de la República Uruguay (University of the Republic Uruguay)
ID : 22320200200121UD
Informations de copyright
© 2023. The Author(s).
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