Transcriptomic Profiling of Embryogenic and Non-Embryogenic Callus Provides New Insight into the Nature of Recalcitrance in Cannabis.
Cannabis sativa
embryonic callus
gene regulation
non-embryogenic callus
plant growth regulators
rooty callus
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
27 Sep 2023
27 Sep 2023
Historique:
received:
31
07
2023
revised:
14
09
2023
accepted:
22
09
2023
medline:
1
11
2023
pubmed:
14
10
2023
entrez:
14
10
2023
Statut:
epublish
Résumé
Differential gene expression profiles of various cannabis calli including non-embryogenic and embryogenic (i.e., rooty and embryonic callus) were examined in this study to enhance our understanding of callus development in cannabis and facilitate the development of improved strategies for plant regeneration and biotechnological applications in this economically valuable crop. A total of 6118 genes displayed significant differential expression, with 1850 genes downregulated and 1873 genes upregulated in embryogenic callus compared to non-embryogenic callus. Notably, 196 phytohormone-related genes exhibited distinctly different expression patterns in the calli types, highlighting the crucial role of plant growth regulator (PGRs) signaling in callus development. Furthermore, 42 classes of transcription factors demonstrated differential expressions among the callus types, suggesting their involvement in the regulation of callus development. The evaluation of epigenetic-related genes revealed the differential expression of 247 genes in all callus types. Notably, histone deacetylases, chromatin remodeling factors, and EMBRYONIC FLOWER 2 emerged as key epigenetic-related genes, displaying upregulation in embryogenic calli compared to non-embryogenic calli. Their upregulation correlated with the repression of embryogenesis-related genes, including LEC2, AGL15, and BBM, presumably inhibiting the transition from embryogenic callus to somatic embryogenesis. These findings underscore the significance of epigenetic regulation in determining the developmental fate of cannabis callus. Generally, our results provide comprehensive insights into gene expression dynamics and molecular mechanisms underlying the development of diverse cannabis calli. The observed repression of auxin-dependent pathway-related genes may contribute to the recalcitrant nature of cannabis, shedding light on the challenges associated with efficient cannabis tissue culture and regeneration protocols.
Identifiants
pubmed: 37834075
pii: ijms241914625
doi: 10.3390/ijms241914625
pmc: PMC10572465
pii:
doi:
Substances chimiques
Plant Growth Regulators
0
Hallucinogens
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Natural Sciences and Engineering Research Council
ID : ALLRP555969-20
Références
Curr Biol. 2013 Jul 22;23(14):1324-9
pubmed: 23810531
Nat Commun. 2019 Aug 2;10(1):3484
pubmed: 31375676
Pest Manag Sci. 2021 Dec;77(12):5356-5366
pubmed: 34309997
Plant Physiol Biochem. 2008 May-Jun;46(5-6):559-68
pubmed: 18406157
Plant J. 2012 Aug;71(3):427-42
pubmed: 22429691
Proteomics. 2018 Mar;18(5-6):e1700265
pubmed: 29369500
Plants (Basel). 2022 Jan 11;11(2):
pubmed: 35050066
Proteomics. 2005 Feb;5(2):461-73
pubmed: 15627954
Int J Mol Sci. 2021 May 26;22(11):
pubmed: 34073522
Plants (Basel). 2021 Jun 14;10(6):
pubmed: 34198660
Nat Protoc. 2009;4(8):1184-91
pubmed: 19617889
Plant Biotechnol J. 2021 Oct;19(10):1979-1987
pubmed: 33960612
Plant Cell. 2007 Jan;19(1):118-30
pubmed: 17259263
Proc Natl Acad Sci U S A. 2006 Feb 28;103(9):3468-73
pubmed: 16492731
Bioinformatics. 2014 Apr 1;30(7):923-30
pubmed: 24227677
Nucleic Acids Res. 2023 Jan 6;51(D1):D445-D451
pubmed: 36350662
Development. 2004 Nov;131(21):5263-76
pubmed: 15456723
J Plant Res. 2015 May;128(3):349-59
pubmed: 25725626
Appl Microbiol Biotechnol. 2021 Jun;105(12):5201-5212
pubmed: 34086118
Regeneration (Oxf). 2017 Dec 05;4(4):201-216
pubmed: 29299323
Bioinformatics. 2017 Apr 15;33(8):1216-1217
pubmed: 28110292
Plants (Basel). 2020 Sep 24;9(10):
pubmed: 32987766
Exp Cell Res. 1968 Apr;50(1):151-8
pubmed: 5650857
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
Genome Biol. 2014;15(12):550
pubmed: 25516281
BMC Genomics. 2021 Feb 27;22(1):140
pubmed: 33639840
BMC Genomics. 2023 Jan 24;24(1):41
pubmed: 36694132
Front Plant Sci. 2019 Jan 08;9:1915
pubmed: 30671070
Front Plant Sci. 2019 Apr 26;10:536
pubmed: 31134106
BMC Biotechnol. 2023 Aug 1;23(1):27
pubmed: 37528396
Curr Opin Plant Biol. 2021 Oct;63:102091
pubmed: 34343847
Front Plant Sci. 2021 Mar 03;12:627240
pubmed: 33747008
Biotechnol Adv. 2023 Jan-Feb;62:108074
pubmed: 36481387
Plant Cell. 2009 Sep;21(9):2563-77
pubmed: 19767455
Front Plant Sci. 2023 Jun 08;14:1154332
pubmed: 37360738
Annu Rev Plant Biol. 2019 Apr 29;70:377-406
pubmed: 30786238
Int J Mol Sci. 2020 Aug 08;21(16):
pubmed: 32784357
Plant Physiol. 2008 Jan;146(1):149-61
pubmed: 18024558
Trends Plant Sci. 2019 Feb;24(2):177-185
pubmed: 30446307
Plant Physiol. 2004 Mar;134(3):995-1005
pubmed: 14963244
Int J Mol Sci. 2021 Aug 09;22(16):
pubmed: 34445260
BMC Plant Biol. 2018 Aug 10;18(1):164
pubmed: 30097018
PLoS Genet. 2011 Mar;7(3):e1002014
pubmed: 21423668
Plant Cell. 2011 Oct;23(10):3671-83
pubmed: 22003076
J Genet Eng Biotechnol. 2020 Jul 13;18(1):31
pubmed: 32661633
Nat Rev Genet. 2022 Jan;23(1):55-68
pubmed: 34526697
Gene. 2019 Feb 5;684:131-138
pubmed: 30321656
PLoS One. 2017 Jul 27;12(7):e0182055
pubmed: 28750086
Trends Plant Sci. 2018 Mar;23(3):235-247
pubmed: 29338924
Hortic Res. 2022 Jan 5;9:
pubmed: 35031801
PLoS Genet. 2017 Jan 17;13(1):e1006562
pubmed: 28095419
Plant Cell Physiol. 2011 Apr;52(4):618-28
pubmed: 21357580
Plant Cell Physiol. 2018 Apr 1;59(4):744-755
pubmed: 29121271
Annu Rev Cell Dev Biol. 2016 Oct 6;32:441-468
pubmed: 27298090
aBIOTECH. 2020 Sep 3;1(3):185-193
pubmed: 36303566
Curr Protoc Bioinformatics. 2015 Sep 03;51:11.14.1-11.14.19
pubmed: 26334920
PLoS One. 2021 Aug 13;16(8):e0235525
pubmed: 34388148
Sci Rep. 2022 Mar 23;12(1):5038
pubmed: 35322147
J Proteomics. 2023 Feb 20;273:104790
pubmed: 36535623
J Exp Bot. 2014 Mar;65(5):1343-60
pubmed: 24622953
Gene. 2022 Jun 5;826:146453
pubmed: 35337851
New Phytol. 2023 May;238(3):1059-1072
pubmed: 36751948
Plants (Basel). 2021 Jan 19;10(1):
pubmed: 33478171
J Cell Biol. 1992 Dec;119(5):1371-80
pubmed: 1280275
Nat Plants. 2015 Jun 29;1:15089
pubmed: 27250255
Dev Cell. 2004 Sep;7(3):373-85
pubmed: 15363412
J Chem Biol. 2009 Nov;2(4):177-90
pubmed: 19763658
Plant J. 2018 Sep;95(6):961-975
pubmed: 29923261
Plant Cell. 2013 Sep;25(9):3159-73
pubmed: 24076977
BMC Genomics. 2014 Jul 04;15:553
pubmed: 24993107
Plant Mol Biol. 2010 Jul;73(4-5):481-92
pubmed: 20405311
Int J Mol Sci. 2020 Mar 26;21(7):
pubmed: 32225116
J Exp Bot. 2023 Feb 13;74(4):1198-1206
pubmed: 34966932
BMC Dev Biol. 2020 Jul 1;20(1):13
pubmed: 32605594
Curr Biol. 2011 Mar 22;21(6):508-14
pubmed: 21396822
Front Plant Sci. 2019 Feb 07;10:77
pubmed: 30792725
Plant J. 2018 Jul;95(2):233-251
pubmed: 29681137
Sci Rep. 2022 Mar 22;12(1):4885
pubmed: 35318409
Cell Res. 2012 Jul;22(7):1169-80
pubmed: 22508267
Front Plant Sci. 2015 Oct 06;6:824
pubmed: 26500668
Biol Futur. 2022 Sep;73(3):259-277
pubmed: 35829936
Development. 2016 May 1;143(9):1442-51
pubmed: 27143753
BMC Dev Biol. 2020 Dec 2;20(1):25
pubmed: 33267776