Identification and characterization of differentially expressed circular RNAs in extraocular muscle of oculomotor nerve palsy.
Circular RNA
Constant exotropia
Extraocular muscle
Oculomotor nerve palsy
Journal
BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258
Informations de publication
Date de publication:
17 Oct 2023
17 Oct 2023
Historique:
received:
09
06
2023
accepted:
11
10
2023
medline:
23
10
2023
pubmed:
18
10
2023
entrez:
17
10
2023
Statut:
epublish
Résumé
Oculomotor nerve palsy (ONP) is a neuroparalytic disorder resulting in dysfunction of innervating extraocular muscles (EOMs), of which the pathological characteristics remain underexplored. In this study, medial rectus muscle tissue samples from four ONP patients and four constant exotropia (CXT) patients were collected for RNA sequencing. Differentially expressed circular RNAs (circRNAs) were identified and included in functional enrichment analysis, followed by interaction analysis with microRNAs and mRNAs as well as RNA binding proteins. Furthermore, RT-qPCR was used to validate the expression level of the differentially expressed circRNAs. A total of 84 differentially expressed circRNAs were identified from 10,504 predicted circRNAs. Functional enrichment analysis indicated that the differentially expressed circRNAs significantly correlated with skeletal muscle contraction. In addition, interaction analyses showed that up-regulated circRNA_03628 was significantly interacted with RNA binding protein AGO2 and EIF4A3 as well as microRNA hsa-miR-188-5p and hsa-miR-4529-5p. The up-regulation of circRNA_03628 was validated by RT-qPCR, followed by further elaboration of the expression, location and clinical significance of circRNA_03628 in EOMs of ONP. Our study may shed light on the role of differentially expressed circRNAs, especially circRNA_03628, in the pathological changes of EOMs in ONP.
Sections du résumé
BACKGROUND
BACKGROUND
Oculomotor nerve palsy (ONP) is a neuroparalytic disorder resulting in dysfunction of innervating extraocular muscles (EOMs), of which the pathological characteristics remain underexplored.
METHODS
METHODS
In this study, medial rectus muscle tissue samples from four ONP patients and four constant exotropia (CXT) patients were collected for RNA sequencing. Differentially expressed circular RNAs (circRNAs) were identified and included in functional enrichment analysis, followed by interaction analysis with microRNAs and mRNAs as well as RNA binding proteins. Furthermore, RT-qPCR was used to validate the expression level of the differentially expressed circRNAs.
RESULTS
RESULTS
A total of 84 differentially expressed circRNAs were identified from 10,504 predicted circRNAs. Functional enrichment analysis indicated that the differentially expressed circRNAs significantly correlated with skeletal muscle contraction. In addition, interaction analyses showed that up-regulated circRNA_03628 was significantly interacted with RNA binding protein AGO2 and EIF4A3 as well as microRNA hsa-miR-188-5p and hsa-miR-4529-5p. The up-regulation of circRNA_03628 was validated by RT-qPCR, followed by further elaboration of the expression, location and clinical significance of circRNA_03628 in EOMs of ONP.
CONCLUSIONS
CONCLUSIONS
Our study may shed light on the role of differentially expressed circRNAs, especially circRNA_03628, in the pathological changes of EOMs in ONP.
Identifiants
pubmed: 37848864
doi: 10.1186/s12864-023-09733-3
pii: 10.1186/s12864-023-09733-3
pmc: PMC10583365
doi:
Substances chimiques
RNA, Circular
0
MicroRNAs
0
RNA, Messenger
0
EIF4A3 protein, human
EC 3.6.1.-
Eukaryotic Initiation Factor-4A
EC 2.7.7.-
DEAD-box RNA Helicases
EC 3.6.4.13
MIRN188 microRNA, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
617Subventions
Organisme : Natural Science Foundation of Shanghai
ID : 20ZR1409800 to LQ. W
Organisme : National Natural Science Foundation of China
ID : 82271126, 81600765 to LQ. W
Organisme : National Natural Science Foundation of China
ID : 82020108006, 81730025, 81670864 and 81525006 to C. Z
Informations de copyright
© 2023. BioMed Central Ltd., part of Springer Nature.
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