Development and application of a universal extraction-free reagent based on an algal glycolipid.
Journal
Analytical methods : advancing methods and applications
ISSN: 1759-9679
Titre abrégé: Anal Methods
Pays: England
ID NLM: 101519733
Informations de publication
Date de publication:
16 11 2023
16 11 2023
Historique:
medline:
17
11
2023
pubmed:
3
11
2023
entrez:
3
11
2023
Statut:
epublish
Résumé
In this study, we independently developed a universal nasopharyngeal swab extraction-free reagent based on a trehalose lipid for the rapid detection of pathogen nucleic acids in respiratory infectious diseases. By comparing the isothermal amplification results of a 2019-nCoV pseudovirus solution treated with different components of the extraction-free reagent, we determined the optimal composition of the extraction-free reagent to be a mixed solution of 10 mmol L
Substances chimiques
Indicators and Reagents
0
Trehalose
B8WCK70T7I
DNA
9007-49-2
Nucleic Acids
0
Lipids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM