Significant influence of four highly conserved amino-acids in lipochaperon-active sHsps on the structure and functions of the Lo18 protein.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
03 11 2023
03 11 2023
Historique:
received:
08
09
2023
accepted:
30
10
2023
medline:
6
11
2023
pubmed:
4
11
2023
entrez:
4
11
2023
Statut:
epublish
Résumé
To cope with environmental stresses, bacteria have developed different strategies, including the production of small heat shock proteins (sHSP). All sHSPs are described for their role as molecular chaperones. Some of them, like the Lo18 protein synthesized by Oenococcus oeni, also have the particularity of acting as a lipochaperon to maintain membrane fluidity in its optimal state following cellular stresses. Lipochaperon activity is poorly characterized and very little information is available on the domains or amino-acids key to this activity. The aim in this paper is to investigate the importance at the protein structure and function level of four highly conserved residues in sHSP exhibiting lipochaperon activity. Thus, by combining in silico, in vitro and in vivo approaches the importance of three amino-acids present in the core of the protein was shown to maintain both the structure of Lo18 and its functions.
Identifiants
pubmed: 37923897
doi: 10.1038/s41598-023-46306-6
pii: 10.1038/s41598-023-46306-6
pmc: PMC10624808
doi:
Substances chimiques
Amino Acids
0
Heat-Shock Proteins, Small
0
Molecular Chaperones
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
19036Informations de copyright
© 2023. The Author(s).
Références
J Appl Microbiol. 2018 Jul;125(1):2-15
pubmed: 29377375
Cell Stress Chaperones. 2019 Sep;24(5):947-956
pubmed: 31338686
FEBS Lett. 2005 Feb 14;579(5):1235-42
pubmed: 15710419
Cell Stress Chaperones. 2021 Jan;26(1):15-18
pubmed: 33083932
Neuroscience. 2008 May 2;153(2):483-91
pubmed: 18384969
Lett Appl Microbiol. 1997 May;24(5):393-6
pubmed: 9172446
Plant Physiol. 2011 Sep;157(1):132-46
pubmed: 21730198
Int J Food Microbiol. 2000 Apr 10;55(1-3):269-73
pubmed: 10791756
J Mol Biol. 2003 May 9;328(4):927-37
pubmed: 12729765
Biochimie. 1996;78(2):117-22
pubmed: 8818220
Protein Sci. 2003 Jun;12(6):1262-70
pubmed: 12761397
Cell Stress Chaperones. 2020 Jul;25(4):593-600
pubmed: 32301005
Annu Rev Microbiol. 2019 Sep 8;73:89-110
pubmed: 31091419
Cell Stress Chaperones. 2023 Jan;28(1):21-33
pubmed: 36367671
Cell Stress Chaperones. 2016 Mar;21(2):327-38
pubmed: 26631139
Methods Mol Biol. 2022;2538:217-234
pubmed: 35951303
Appl Microbiol Biotechnol. 2001 Oct;57(1-2):34-42
pubmed: 11693931
Appl Environ Microbiol. 1997 Feb;63(2):609-14
pubmed: 9023938
Can J Biochem Physiol. 1959 Aug;37(8):911-7
pubmed: 13671378
Biochem Biophys Res Commun. 1997 Jul 18;236(2):370-4
pubmed: 9240443
Biochim Biophys Acta. 1978 Dec 15;515(4):367-94
pubmed: 365237
Nat Commun. 2019 Mar 6;10(1):1068
pubmed: 30842409
Cell Stress Chaperones. 2023 Jan;28(1):79-89
pubmed: 36417097
Nature. 1952 Jul 12;170(4315):53-5
pubmed: 14957017
Genomics. 2021 Jul;113(4):2812-2825
pubmed: 34129932
Biochim Biophys Acta. 2005 Dec 30;1720(1-2):92-8
pubmed: 16472556
Infect Immun. 1992 May;60(5):2066-74
pubmed: 1563797
Nucleic Acids Res. 2022 Jul 5;50(W1):W90-W98
pubmed: 35544232
J Biol Chem. 1993 Jan 25;268(3):1517-20
pubmed: 8093612
FEMS Microbiol Lett. 2010 Aug 1;309(1):8-15
pubmed: 20546310
Curr Mol Med. 2015;15(1):38-46
pubmed: 25601467
Appl Environ Microbiol. 1989 Mar;55(3):751-3
pubmed: 16347882
Acc Chem Res. 2016 Feb 16;49(2):184-92
pubmed: 26756718
J Bacteriol. 2005 Aug;187(15):5236-41
pubmed: 16030217
Anal Biochem. 2004 Dec 15;335(2):338-9
pubmed: 15556575
Front Pharmacol. 2019 Sep 18;10:1047
pubmed: 31619995
J Mol Biol. 2015 Apr 10;427(7):1537-48
pubmed: 25681016
Fold Des. 1996;1(2):91-101
pubmed: 9079369
J Biol Chem. 1996 Mar 22;271(12):7218-23
pubmed: 8636160
J Biol Chem. 2005 Jun 24;280(25):23861-8
pubmed: 15843375
Nucleic Acids Res. 2018 Jul 2;46(W1):W296-W303
pubmed: 29788355
Appl Environ Microbiol. 2014 May;80(10):2973-80
pubmed: 24584255
Appl Microbiol Biotechnol. 2019 Sep;103(17):6867-6883
pubmed: 31300854
J Mol Biol. 2004 Oct 15;343(2):435-44
pubmed: 15451671
J Mol Microbiol Biotechnol. 2001 Oct;3(4):601-10
pubmed: 11545277
Biophys J. 2012 Oct 3;103(7):1460-9
pubmed: 23062338
Proc Natl Acad Sci U S A. 1982 Apr;79(7):2360-4
pubmed: 6285380
J Bacteriol. 2004 Apr;186(7):2200-5
pubmed: 15028706
Biochemistry (Mosc). 2017 Feb;82(2):106-121
pubmed: 28320295
FEBS Lett. 1995 Sep 25;372(2-3):283-7
pubmed: 7556685
Biochem J. 2012 May 15;444(1):97-104
pubmed: 22360742
Biochem Biophys Res Commun. 2005 May 20;330(4):1055-61
pubmed: 15823550
J Biol Chem. 2018 Dec 21;293(51):19511-19521
pubmed: 30348902
Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3098-103
pubmed: 11248038