Turning waste into treasure: Biosynthesis of value-added 2-O-α-glucosyl glycerol and d-allulose from waste cane molasses through an in vitro synthetic biology platform.
Bienzyme system
Glycosides
Rare sugars
Sucrose phosphorylase
d-allulose 3-epimerase
Journal
Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523
Informations de publication
Date de publication:
Jan 2024
Jan 2024
Historique:
received:
14
09
2023
revised:
02
11
2023
accepted:
02
11
2023
medline:
23
11
2023
pubmed:
6
11
2023
entrez:
5
11
2023
Statut:
ppublish
Résumé
The efficient and economical conversion of agricultural waste into glycosides and rare sugars is challenging. Herein, an in vitro synthetic bienzyme system consisting of sucrose phosphorylase and d-allulose 3-epimerase was constructed to produce 2-O-α-glucosyl glycerol and d-allulose from cane molasses. Lactic acid in the cane molasses significantly induced sucrose phosphorylase to hydrolyze sucrose instead of glycosylation. Notably, lactic acid significantly inhibited the catalytic performance of d-allulose 3-epimerase only in the presence of Na
Identifiants
pubmed: 37926357
pii: S0960-8524(23)01410-4
doi: 10.1016/j.biortech.2023.129982
pii:
doi:
Substances chimiques
psicose
23140-52-5
Glycerol
PDC6A3C0OX
Fructose
30237-26-4
Sucrose
57-50-1
Lactic Acid
33X04XA5AT
Racemases and Epimerases
EC 5.1.-
Glycosides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
129982Informations de copyright
Copyright © 2023 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.