Dual stop codon suppression in mammalian cells with genomically integrated genetic code expansion machinery.

Codon, Terminator / genetics Genetic Code / genetics RNA, Transfer / genetics Amino Acids / genetics Amino Acyl-tRNA Synthetases / genetics
CP: Biotechnology CP: Molecular biology Genetic code expansion amber suppression bioorthogonal labeling click chemistry mammalian cell culture non-canonical amino acids random genomic integration stop codon suppression unnatural amino acids

Journal

Cell reports methods
ISSN: 2667-2375
Titre abrégé: Cell Rep Methods
Pays: United States
ID NLM: 9918227360606676

Informations de publication

Date de publication:
20 Nov 2023
Historique:
received: 22 04 2023
revised: 22 08 2023
accepted: 12 10 2023
medline: 27 11 2023
pubmed: 8 11 2023
entrez: 7 11 2023
Statut: ppublish

Résumé

Stop codon suppression using dedicated tRNA/aminoacyl-tRNA synthetase (aaRS) pairs allows for genetically encoded, site-specific incorporation of non-canonical amino acids (ncAAs) as chemical handles for protein labeling and modification. Here, we demonstrate that piggyBac-mediated genomic integration of archaeal pyrrolysine tRNA (tRNA

Identifiants

DOI: 10.1016/j.crmeth.2023.100626 PMID: 37935196 PMC: PMC10694491
pubmed: 37935196
pii: S2667-2375(23)00290-4
doi: 10.1016/j.crmeth.2023.100626
pmc: PMC10694491
pii:
doi:

Substances chimiques

Codon, Terminator 0
RNA, Transfer 9014-25-9
Amino Acids 0
Amino Acyl-tRNA Synthetases EC 6.1.1.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

100626

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

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Auteurs

Birthe Meineke (B)

Science for Life Laboratory, Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Genome Biology, 17165 Stockholm, Sweden; Ming Wai Lau Centre for Reparative Medicine, Stockholm Node, Karolinska Institutet, 17165 Stockholm, Sweden. Electronic address: birthe.meineke@scilifelab.se.

Johannes Heimgärtner (J)

Science for Life Laboratory, Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Genome Biology, 17165 Stockholm, Sweden; Ming Wai Lau Centre for Reparative Medicine, Stockholm Node, Karolinska Institutet, 17165 Stockholm, Sweden.

Rozina Caridha (R)

Science for Life Laboratory, Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Genome Biology, 17165 Stockholm, Sweden; Ming Wai Lau Centre for Reparative Medicine, Stockholm Node, Karolinska Institutet, 17165 Stockholm, Sweden.

Matthias F Block (MF)

Science for Life Laboratory, Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Genome Biology, 17165 Stockholm, Sweden.

Kyle J Kimler (KJ)

Science for Life Laboratory, Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Genome Biology, 17165 Stockholm, Sweden.

Maria F Pires (MF)

Science for Life Laboratory, Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Genome Biology, 17165 Stockholm, Sweden.

Michael Landreh (M)

Department of Microbiology, Tumor and Cell Biology, Science for Life Laboratory, Karolinska Institutet, 17165 Stockholm, Sweden.

Simon J Elsässer (SJ)

Science for Life Laboratory, Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Genome Biology, 17165 Stockholm, Sweden; Ming Wai Lau Centre for Reparative Medicine, Stockholm Node, Karolinska Institutet, 17165 Stockholm, Sweden. Electronic address: simon.elsasser@scilifelab.se.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Codon › Codon stop › Dual stop codon suppression in mammalian cells...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Code génétique › Dual stop codon suppression in mammalian cells...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN de transfert › Dual stop codon suppression in mammalian cells...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Dual stop codon suppression in mammalian cells...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Ligases › Carbon-oxygen ligases › Amino acyl-tRNA synthetases › Dual stop codon suppression in mammalian cells...