Coordinated regulation of the entry and exit steps of aromatic amino acid biosynthesis supports the dual lignin pathway in grasses.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
09 11 2023
09 11 2023
Historique:
received:
16
03
2023
accepted:
16
10
2023
medline:
13
11
2023
pubmed:
10
11
2023
entrez:
9
11
2023
Statut:
epublish
Résumé
Vascular plants direct large amounts of carbon to produce the aromatic amino acid phenylalanine to support the production of lignin and other phenylpropanoids. Uniquely, grasses, which include many major crops, can synthesize lignin and phenylpropanoids from both phenylalanine and tyrosine. However, how grasses regulate aromatic amino acid biosynthesis to feed this dual lignin pathway is unknown. Here we show, by stable-isotope labeling, that grasses produce tyrosine >10-times faster than Arabidopsis without compromising phenylalanine biosynthesis. Detailed in vitro enzyme characterization and combinatorial in planta expression uncovered that coordinated expression of specific enzyme isoforms at the entry and exit steps of the aromatic amino acid pathway enables grasses to maintain high production of both tyrosine and phenylalanine, the precursors of the dual lignin pathway. These findings highlight the complex regulation of plant aromatic amino acid biosynthesis and provide novel genetic tools to engineer the interface of primary and specialized metabolism in plants.
Identifiants
pubmed: 37945591
doi: 10.1038/s41467-023-42587-7
pii: 10.1038/s41467-023-42587-7
pmc: PMC10636026
doi:
Substances chimiques
Lignin
9005-53-2
Amino Acids, Aromatic
0
Phenylalanine
47E5O17Y3R
Tyrosine
42HK56048U
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
7242Informations de copyright
© 2023. The Author(s).
Références
Plant Cell. 2005 May;17(5):1612-24
pubmed: 15805488
Trends Plant Sci. 2020 Jan;25(1):66-79
pubmed: 31679994
Proc Natl Acad Sci U S A. 1986 Sep;83(18):6873-7
pubmed: 16593759
Nat Plants. 2016 Jun 03;2(6):16080
pubmed: 27255843
Planta. 1982 Dec;156(3):233-40
pubmed: 24272471
Front Plant Sci. 2013 Mar 27;4:62
pubmed: 23543266
Plant J. 2003 Nov;36(3):301-17
pubmed: 14617088
Plant J. 2022 Feb;109(4):844-855
pubmed: 34807484
Biochemistry. 1984 Dec 4;23(25):6240-9
pubmed: 6395895
Curr Opin Plant Biol. 2022 Jun;67:102219
pubmed: 35550985
Annu Rev Plant Biol. 2003;54:519-46
pubmed: 14503002
Plant Cell. 2017 Aug;29(8):1806-1821
pubmed: 28808136
Annu Rev Plant Biol. 2021 Jun 17;72:185-216
pubmed: 33848429
Phytochemistry. 2018 May;149:82-102
pubmed: 29477627
Prep Biochem Biotechnol. 2004 Aug;34(3):209-14
pubmed: 15461137
Plant Physiol. 2004 Oct;136(2):3058-69
pubmed: 15448200
Front Plant Sci. 2012 May 24;3:100
pubmed: 22654888
Plant Cell. 2011 Jul;23(7):2738-53
pubmed: 21750236
Plant Physiol. 2001 Mar;125(3):1198-205
pubmed: 11244101
Plant Physiol. 2018 Feb;176(2):1452-1468
pubmed: 29196539
Nat Chem Biol. 2011 Jan;7(1):19-21
pubmed: 21102469
PLoS One. 2011 Feb 18;6(2):e16765
pubmed: 21364738
Front Plant Sci. 2021 Jan 12;11:604349
pubmed: 33510749
Nat Chem Biol. 2015 Jan;11(1):52-7
pubmed: 25402771
New Phytol. 2018 Jan;217(2):896-908
pubmed: 28990194
Plant Cell. 2021 May 5;33(3):671-696
pubmed: 33955484
Plant Cell. 1995 Jul;7(7):907-919
pubmed: 12242393
Plant Physiol. 2016 Mar;170(3):1878-94
pubmed: 26754669
Plant Physiol. 1972 Oct;50(4):480-4
pubmed: 16658200
Nat Plants. 2016 May 09;2(6):16050
pubmed: 27255834
J Biol Chem. 2010 Oct 1;285(40):30567-76
pubmed: 20667835
Mol Plant. 2010 Nov;3(6):956-72
pubmed: 20817774
Nat Chem Biol. 2017 Sep;13(9):1029-1035
pubmed: 28671678
Phytochemistry. 2004 Jun;65(11):1557-64
pubmed: 15276452
Trends Plant Sci. 2020 Jul;25(7):670-681
pubmed: 32526172
Appl Environ Microbiol. 2005 Nov;71(11):7224-8
pubmed: 16269762
Nat Commun. 2019 Jan 3;10(1):15
pubmed: 30604768
Plant Cell Physiol. 2015 Jan;56(1):e6
pubmed: 25505007
BMC Plant Biol. 2008 Nov 07;8:112
pubmed: 18992143
Eur J Biochem. 2002 Oct;269(19):4753-61
pubmed: 12354106
Sci Adv. 2022 Jun 10;8(23):eabo3416
pubmed: 35675400
Plant Physiol. 2004 Dec;136(4):4326-34
pubmed: 15531710
Plant Physiol. 1997 Jan;113(1):175-9
pubmed: 9008393
Plant Biotechnol J. 2015 Jan;13(1):125-36
pubmed: 25283446
Front Plant Sci. 2015 Jul 16;6:538
pubmed: 26236327
Methods Mol Biol. 2017;1653:157-166
pubmed: 28822132
Plant J. 2019 Mar;97(5):901-922
pubmed: 30457178
Plant Cell. 2010 Mar;22(3):832-49
pubmed: 20215586
Plant Cell Physiol. 2018 Jan 1;59(1):e3
pubmed: 29216398
J Biol Chem. 1976 Sep 25;251(18):5440-7
pubmed: 9387
Plant J. 2021 Nov;108(3):737-751
pubmed: 34403557
ACS Synth Biol. 2014 Nov 21;3(11):839-43
pubmed: 24933124
Plant Physiol. 2022 Jan 20;188(1):134-150
pubmed: 34633048
Proc Natl Acad Sci U S A. 1986 Oct;83(19):7231-5
pubmed: 3463961
Front Plant Sci. 2021 Sep 14;12:714164
pubmed: 34594350
J Biol Chem. 2012 Mar 30;287(14):11446-59
pubmed: 22311980
Mol Biol Evol. 2021 Jun 25;38(7):3022-3027
pubmed: 33892491
Mol Plant. 2010 Jan;3(1):2-20
pubmed: 20035037
Nucleic Acids Res. 2012 Jan;40(Database issue):D1178-86
pubmed: 22110026
New Phytol. 2012 Apr;194(2):430-439
pubmed: 22296303
Nucleic Acids Res. 2009 Apr;37(6):e45
pubmed: 19237396
Annu Rev Plant Biol. 2012;63:73-105
pubmed: 22554242
Z Naturforsch C J Biosci. 1986 Jan-Feb;41(1-2):69-78
pubmed: 2939643