Reshaping the Substrate Binding Pocket of β-Amino Acid Dehydrogenase for the Synthesis of Aromatic β-Amino Acids.

Amino Acids, Aromatic / metabolism Amino Acid Oxidoreductases / chemistry Amino Acids / metabolism Amination Keto Acids Substrate Specificity

Journal

Organic letters
ISSN: 1523-7052
Titre abrégé: Org Lett
Pays: United States
ID NLM: 100890393

Informations de publication

Date de publication:
01 12 2023
Historique:
medline: 4 12 2023
pubmed: 17 11 2023
entrez: 16 11 2023
Statut: ppublish

Résumé

By reshaping the substrate-binding pocket of β-amino acid dehydrogenase (β-AADH), some variants were obtained with up to 2560-fold enhanced activity toward the model substrates (

Identifiants

DOI: 10.1021/acs.orglett.3c03366 PMID: 37972311
pubmed: 37972311
doi: 10.1021/acs.orglett.3c03366
doi:

Substances chimiques

Amino Acids, Aromatic 0
Amino Acid Oxidoreductases EC 1.4.-
Amino Acids 0
Keto Acids 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

8469-8473

Auteurs

Na Liu (N)

National Engineering Research Center of Industrial Enzymes, Tianjin Engineering Research Center of Biocatalytic Technology, Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, National Center of Technology Innovation for Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.

Jinhui Feng (J)

National Engineering Research Center of Industrial Enzymes, Tianjin Engineering Research Center of Biocatalytic Technology, Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, National Center of Technology Innovation for Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
ORCID: 0000-0001-9320-7323

Xi Chen (X)

National Engineering Research Center of Industrial Enzymes, Tianjin Engineering Research Center of Biocatalytic Technology, Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, National Center of Technology Innovation for Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
ORCID: 0000-0002-1530-8353

Yuyang Luo (Y)

National Engineering Research Center of Industrial Enzymes, Tianjin Engineering Research Center of Biocatalytic Technology, Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, National Center of Technology Innovation for Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
School of Biotechnology, Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.

Tong Lv (T)

National Engineering Research Center of Industrial Enzymes, Tianjin Engineering Research Center of Biocatalytic Technology, Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, National Center of Technology Innovation for Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.

Qiaqing Wu (Q)

National Engineering Research Center of Industrial Enzymes, Tianjin Engineering Research Center of Biocatalytic Technology, Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, National Center of Technology Innovation for Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
ORCID: 0000-0001-7405-1562

Dunming Zhu (D)

National Engineering Research Center of Industrial Enzymes, Tianjin Engineering Research Center of Biocatalytic Technology, Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, National Center of Technology Innovation for Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
ORCID: 0000-0001-8783-5358

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés cycliques › Acides aminés aromatiques › Reshaping the Substrate Binding Pocket of...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Oxidoreductases acting on CH-NH2 group donors › Amino-acid oxidoreductases › Reshaping the Substrate Binding Pocket of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Reshaping the Substrate Binding Pocket of...
questionsmedicales.fr › Métabolisme › Amination › Reshaping the Substrate Binding Pocket of...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Cétoacides › Reshaping the Substrate Binding Pocket of...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Spécificité du substrat › Reshaping the Substrate Binding Pocket of...