Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
22 Nov 2023
22 Nov 2023
Historique:
received:
19
12
2022
accepted:
03
11
2023
medline:
24
11
2023
pubmed:
23
11
2023
entrez:
22
11
2023
Statut:
epublish
Résumé
While mesenchymal stem cells (MSCs) have gained enormous attention due to their unique properties of self-renewal, colony formation, and differentiation potential, the MSC secretome has become attractive due to its roles in immunomodulation, anti-inflammatory activity, angiogenesis, and anti-apoptosis. However, the precise stimulation and efficient production of the MSC secretome for therapeutic applications are challenging problems to solve. Here, we report on Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs: AIMS. We create an acoustofluidic mechanobiological environment to form reproducible three-dimensional MSC aggregates, which produce the MSC secretome with high efficiency. We confirm the increased MSC secretome is due to improved cell-cell interactions using AIMS: the key mediator N-cadherin was up-regulated while functional blocking of N-cadherin resulted in no enhancement of the secretome. After being primed by IFN-γ, the secretome profile of the MSC aggregates contains more anti-inflammatory cytokines and can be used to inhibit the pro-inflammatory response of M1 phenotype macrophages, suppress T cell activation, and support B cell functions. As such, the MSC secretome can be modified for personalized secretome-based therapies. AIMS acts as a powerful tool for improving the MSC secretome and precisely tuning the secretory profile to develop new treatments in translational medicine.
Identifiants
pubmed: 37993431
doi: 10.1038/s41467-023-43239-6
pii: 10.1038/s41467-023-43239-6
pmc: PMC10665559
doi:
Substances chimiques
Cytokines
0
Anti-Inflammatory Agents
0
Cadherins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7639Subventions
Organisme : NCI NIH HHS
ID : R33 CA223908
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM141055
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM145960
Pays : United States
Informations de copyright
© 2023. The Author(s).
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