Reporter Gene-Based qRT-PCR Assay for Rho-Dependent Termination In Vivo.
Rho-dependent termination
operon
qRT-PCR
reporter gene
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
09 Nov 2023
09 Nov 2023
Historique:
received:
15
09
2023
revised:
06
11
2023
accepted:
07
11
2023
medline:
27
11
2023
pubmed:
24
11
2023
entrez:
24
11
2023
Statut:
epublish
Résumé
In bacteria, the Rho protein mediates Rho-dependent termination (RDT) by identifying a non-specific cytosine-rich Rho utilization site on the newly synthesized RNA. As a result of RDT, downstream RNA transcription is reduced. Due to the bias in reverse transcription and PCR amplification, we could not identify the RDT site by directly measuring the amount of mRNA upstream and downstream of RDT sites. To overcome this difficulty, we employed a 77 bp reporter gene
Identifiants
pubmed: 37998331
pii: cells12222596
doi: 10.3390/cells12222596
pmc: PMC10670590
pii:
doi:
Substances chimiques
RNA, Transfer, Arg
0
RNA
63231-63-0
RNA, Messenger
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Key Research and Development Program of China
ID : 2022YFF1000700
Organisme : National Natural Science Foundation of China
ID : 31971339
Organisme : National Natural Science Foundation of China
ID : 32171422
Organisme : Fundamental Research Funds for the Central Universities
ID : 2662022SKYJ004
Organisme : Chungnam National University
ID : 2020
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