Characterization of Klebsiella pneumoniae Extracellular Polysaccharides.
Klebsiella pneumoniae
capsule
extracellular polysaccharide
hypermucoviscosity
silver stain
Journal
Current protocols
ISSN: 2691-1299
Titre abrégé: Curr Protoc
Pays: United States
ID NLM: 101773894
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
pmc-release:
01
11
2024
medline:
29
11
2023
pubmed:
27
11
2023
entrez:
27
11
2023
Statut:
ppublish
Résumé
Klebsiella pneumoniae is a clinically significant, Gram-negative pathogen in which the production of extracellular polysaccharides is a key virulence factor. Extracellular polysaccharides such as the capsule and its mucoviscosity play a significant role in K. pneumoniae infection. In this article, we explain several standard protocols used to characterize the extracellular polysaccharides of K. pneumoniae. Several of these protocols are adapted specifically for K. pneumoniae and describe methods to purify and quantify the extracellular polysaccharide of K. pneumoniae. We also present a standardized protocol to quantify K. pneumoniae mucoviscosity, a unique feature of K. pneumoniae extracellular polysaccharide. These protocols will help create uniformity in standard protocols used in K. pneumoniae extracellular polysaccharide studies. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Extracellular polysaccharide isolation and purification Basic Protocol 2: Large-scale isolation and purification of extracellular polysaccharide Basic Protocol 3: Uronic acid quantification of extracellular polysaccharide Basic Protocol 4: Extracellular polysaccharide visualization by SDS-PAGE Basic Protocol 5: Klebsiella pneumoniae mucoviscosity measurement by sedimentation resistance assay Alternate Protocol 5: 96-well plate-based Klebsiella pneumoniae sedimentation resistance assay Support Protocol 5: Determination of plate to cuvette conversion factor.
Identifiants
pubmed: 38010271
doi: 10.1002/cpz1.937
pmc: PMC10683871
mid: NIHMS1942868
doi:
Substances chimiques
Polysaccharides
0
Virulence Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e937Subventions
Organisme : American Heart Association-American Stroke Association
ID : 23CDA1056712
Pays : United States
Organisme : NIAID NIH HHS
ID : K22 AI145849
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM150588
Pays : United States
Informations de copyright
© 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.
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