Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
27 Nov 2023
27 Nov 2023
Historique:
received:
16
05
2023
accepted:
15
11
2023
medline:
29
11
2023
pubmed:
28
11
2023
entrez:
27
11
2023
Statut:
epublish
Résumé
Formic acid (FA) has emerged as a promising one-carbon feedstock for biorefinery. However, developing efficient microbial hosts for economically competitive FA utilization remains a grand challenge. Here, we discover that the bacterium Vibrio natriegens has exceptional FA tolerance and metabolic capacity natively. This bacterium is remodeled by rewiring the serine cycle and the TCA cycle, resulting in a non-native closed loop (S-TCA) which as a powerful metabolic sink, in combination with laboratory evolution, enables rapid emergence of synthetic strains with significantly improved FA-utilizing ability. Further introduction of a foreign indigoidine-forming pathway into the synthetic V. natriegens strain leads to the production of 29.0 g · L
Identifiants
pubmed: 38012202
doi: 10.1038/s41467-023-43631-2
pii: 10.1038/s41467-023-43631-2
pmc: PMC10682008
doi:
Substances chimiques
formic acid
0YIW783RG1
Formates
0
Carbon
7440-44-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7758Informations de copyright
© 2023. The Author(s).
Références
Cell Biochem Biophys. 2014 Apr;68(3):475-8
pubmed: 24068518
Trends Biotechnol. 2016 Mar;34(3):191-197
pubmed: 26702790
Proc Natl Acad Sci U S A. 2018 Oct 2;115(40):E9271-E9279
pubmed: 30224468
FEMS Microbiol Ecol. 2016 May;92(5):fiw069
pubmed: 27037359
Front Microbiol. 2021 Feb 19;12:627181
pubmed: 33679648
J Bacteriol. 2003 Apr;185(8):2548-54
pubmed: 12670979
Nat Microbiol. 2020 Dec;5(12):1459-1463
pubmed: 32989263
ACS Synth Biol. 2019 May 17;8(5):911-917
pubmed: 31002757
Environ Microbiol. 2020 Oct;22(10):4394-4408
pubmed: 32537803
J Bacteriol. 1999 Jul;181(13):3890-7
pubmed: 10383954
Biochemistry. 2016 Jul 19;55(28):3851-63
pubmed: 27348189
Proc Natl Acad Sci U S A. 2014 Apr 29;111(17):6419-24
pubmed: 24733917
J Bacteriol. 2003 Nov;185(21):6448-55
pubmed: 14563880
J Bacteriol. 1962 Apr;83:879-86
pubmed: 13888944
Acta Biochim Biophys Sin (Shanghai). 2021 Apr 15;53(5):620-627
pubmed: 33764372
Nat Commun. 2020 Oct 23;11(1):5385
pubmed: 33097726
Nat Commun. 2023 May 9;14(1):2682
pubmed: 37160875
Microb Cell Fact. 2005 Aug 25;4:25
pubmed: 16122392
Nat Chem Biol. 2020 May;16(5):538-545
pubmed: 32042198
ACS Synth Biol. 2022 Jan 21;11(1):135-143
pubmed: 34979802
Anticancer Res. 2005 Jan-Feb;25(1A):59-62
pubmed: 15816519
Microorganisms. 2020 Mar 23;8(3):
pubmed: 32210133
Appl Environ Microbiol. 2017 Oct 31;83(22):
pubmed: 28887417
ISME J. 2021 Mar;15(3):673-687
pubmed: 33082573
Synth Biol (Oxf). 2021 Aug 06;6(1):ysab020
pubmed: 34651085
Microb Biotechnol. 2015 Jan;8(1):155-63
pubmed: 25123319
Nat Methods. 2016 Oct;13(10):849-51
pubmed: 27571549
Nat Commun. 2020 Mar 20;11(1):1496
pubmed: 32198415
J Bacteriol. 2011 Mar;193(6):1393-404
pubmed: 21216997
Metab Eng. 2021 May;65:52-65
pubmed: 33722653
Front Microbiol. 2020 Jan 31;11:58
pubmed: 32082285
Nucleic Acids Res. 2019 Jan 8;47(D1):D1164-D1171
pubmed: 30357390
J Bacteriol. 1969 Nov;100(2):890-4
pubmed: 5354954
Biotechnol Biofuels. 2017 Jan 26;10:22
pubmed: 28149324
Adv Sci (Weinh). 2021 May 03;8(12):2100199
pubmed: 34194943
Essays Biochem. 2021 Jul 26;65(2):381-392
pubmed: 33835156
J Bacteriol. 1975 Apr;122(1):47-53
pubmed: 235511
Nat Commun. 2020 Oct 9;11(1):5090
pubmed: 33037220
ACS Synth Biol. 2017 Sep 15;6(9):1650-1655
pubmed: 28571309
Biochem J. 1983 Dec 15;216(3):709-16
pubmed: 6365081
J Bacteriol. 2016 Dec 13;199(1):
pubmed: 27795317
Nat Microbiol. 2019 Jul;4(7):1105-1113
pubmed: 30962569
ACS Synth Biol. 2018 Sep 21;7(9):2023-2028
pubmed: 29763299
Mol Cell Proteomics. 2012 Jun;11(6):M111.015420
pubmed: 22232491
Nat Chem Biol. 2021 Aug;17(8):845-855
pubmed: 34312558
ACS Synth Biol. 2017 Sep 15;6(9):1722-1731
pubmed: 28558223