Programming crystallization kinetics of self-assembled DNA crystals with 5-methylcytosine modification.
5-methylcytosine
DNA crystal
DNA nanotechnology
crystallization
nucleobase chemical modification
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
12 Mar 2024
12 Mar 2024
Historique:
medline:
6
3
2024
pubmed:
4
3
2024
entrez:
4
3
2024
Statut:
ppublish
Résumé
Self-assembled DNA crystals offer a precise chemical platform at the ångström-scale for DNA nanotechnology, holding enormous potential in material separation, catalysis, and DNA data storage. However, accurately controlling the crystallization kinetics of such DNA crystals remains challenging. Herein, we found that atomic-level 5-methylcytosine (5mC) modification can regulate the crystallization kinetics of DNA crystal by tuning the hybridization rates of DNA motifs. We discovered that by manipulating the axial and combination of 5mC modification on the sticky ends of DNA tensegrity triangle motifs, we can obtain a series of DNA crystals with controllable morphological features. Through DNA-PAINT and FRET-labeled DNA strand displacement experiments, we elucidate that atomic-level 5mC modification enhances the affinity constant of DNA hybridization at both the single-molecule and macroscopic scales. This enhancement can be harnessed for kinetic-driven control of the preferential growth direction of DNA crystals. The 5mC modification strategy can overcome the limitations of DNA sequence design imposed by limited nucleobase numbers in various DNA hybridization reactions. This strategy provides a new avenue for the manipulation of DNA crystal structure, valuable for the advancement of DNA and biomacromolecular crystallography.
Identifiants
pubmed: 38437555
doi: 10.1073/pnas.2312596121
doi:
Substances chimiques
5-Methylcytosine
6R795CQT4H
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2312596121Subventions
Organisme : the National Nature Science Foundation of China
ID : T2188102 21991134
Organisme : the National Nature Science Founation of China
ID : 92056117 21934007
Organisme : the National Research Programs from the Ministry of Science and Technology of China
ID : 2022YFF0710000
Organisme : the National Nature Science Foundation of China
ID : 22122406
Organisme : the National Key R&D Program of China
ID : 2022YFF1201801
Organisme : the China Postdoctoral Science Foundation
ID : 2021M702106
Organisme : the China Postdoctoral Science Foundation
ID : 2022M722710
Déclaration de conflit d'intérêts
Competing interests statement:The authors declare no competing interest.
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