Agroinfiltration for Enhanced Transgene Expression in Coffee Leaves (Coffea arabica L.).
Agrobacterium-mediated transformation
Gene function analysis
Genetic engineering
Leaf infiltration
Transgene expression
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2024
2024
Historique:
medline:
24
4
2024
pubmed:
24
4
2024
entrez:
24
4
2024
Statut:
ppublish
Résumé
The Coffea spp. plant is a significant crop in Latin America, Africa, and Asia, and recent advances in genomics and transcriptomics have opened possibilities for studying candidate genes and introducing new desirable traits through genetic engineering. While stable transformation of coffee plants has been reported using various techniques, it is a time-consuming and laborious process. To overcome this, transient transformation methods have been developed, which avoid the limitations of stable transformation. This chapter describes an ex vitro protocol for transient expression using A. tumefaciens-mediated infiltration of coffee leaves, which could be used to produce coffee plants expressing desirable traits against biotic and abiotic stresses, genes controlling biochemical and physiological traits, as well as for gene editing through CRISPR/Cas9.
Identifiants
pubmed: 38656517
doi: 10.1007/978-1-0716-3782-1_13
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
227-241Informations de copyright
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Ariyoshi C, Freitas de Oliveira F, Harumi Shigueoka L, Garbossi da Silva A, Gatica Arias A, Villalta-Villalobos J, Ivamoto-Suzuki ST, Hiroshi Sera G, Teixeira Caixeta E, da Rocha MR, Bucker Moraes W, Partelli Fábio L, Protasio Pereira LF (2022) Current challenges and genomic advances toward the development of coffee genotypes resistant to biotic stress. In: Kole C (ed) Genomic designing for biotic stress resistant technical crops. Springer, Cham. https://doi.org/10.1007/978-3-031-09293-0_4
doi: 10.1007/978-3-031-09293-0_4
Cao DV, Pamplona RS, Kim J, Oh YK, Cho SK, Ahn J, Yang SW, Riu KZ, Boo KH (2017) Optimization of Agrobacterium-mediated transient expression of heterologous genes in spinach. Plant Biotechnol Rep. https://doi.org/10.1007/s11816-017-0457-4
Zhao H, Tan Z, Wen X, Wang Y (2017) An improved syringe agroinfiltration protocol to enhance transformation efficiency by combinative use of 5-azacytidine, ascorbate acid and tween-20. Plan Theory 6:9. https://doi.org/10.3390/plants6010009
doi: 10.3390/plants6010009
Ribas AF, Dechamp E, Champion A, Bertrand B, Combes MC, Verdeil JL, Lapeyre F, Lashermes P, Etienne H (2011) Agrobacterium mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures. BMC Plant 11:92. https://doi.org/10.1186/1471-2229-11-92
doi: 10.1186/1471-2229-11-92
Villalta-Villalobos J, Gatica-Arias A (2019) Una mirada en el tiempo: mejoramiento genético de café mediante la aplicación de la biotecnología. Agron Mesoam 30(2):577–599. https://doi.org/10.15517/am.v30i2.34173
doi: 10.15517/am.v30i2.34173
Acanda Y, Welker S, Orbović V et al (2021) A simple and efficient agroinfiltration method for transient gene expression in Citrus. Plant Cell Rep 40:1171–1179. https://doi.org/10.1007/s00299-021-02700-w
doi: 10.1007/s00299-021-02700-w
pubmed: 33948685