Synergistic activity of gold nanoparticles with amphotericin B on persister cells of Candida tropicalis biofilms.


Journal

Journal of nanobiotechnology
ISSN: 1477-3155
Titre abrégé: J Nanobiotechnology
Pays: England
ID NLM: 101152208

Informations de publication

Date de publication:
16 May 2024
Historique:
received: 23 10 2023
accepted: 18 03 2024
medline: 17 5 2024
pubmed: 17 5 2024
entrez: 16 5 2024
Statut: epublish

Résumé

The antifungal activity was studied on sessile and persister cells (PCs) of Candida tropicalis biofilms of gold nanoparticles (AuNPs) stabilized with cetyltrimethylammonium bromide (CTAB-AuNPs) and those conjugated with cysteine, in combination with Amphotericin B (AmB). The PC model was used and synergistic activity was tested by the checkerboard assay. Biofilms were studied by crystal violet and scanning electron microscopy. After the combination of both AuNPs and AmB the biofilm biomass was reduced, with significant differences in architecture being observed with a reduced biofilm matrix. In addition, the CTAB-AuNPs-AmB combination significantly reduced PCs. Understanding how these AuNPs aid in the fight against biofilms and the development of new approaches to eradicate PCs has relevance for chronic infection treatment.

Identifiants

pubmed: 38755625
doi: 10.1186/s12951-024-02415-6
pii: 10.1186/s12951-024-02415-6
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

254

Subventions

Organisme : Consejo Nacional de Investigaciones Científicas y Técnicas
ID : PIP
Organisme : Consejo Nacional de Investigaciones Científicas y Técnicas
ID : PIP
Organisme : Consejo Nacional de Investigaciones Científicas y Técnicas
ID : PIP
Organisme : Consejo Nacional de Investigaciones Científicas y Técnicas
ID : PIP
Organisme : Consejo Nacional de Investigaciones Científicas y Técnicas
ID : PIP
Organisme : Consejo Nacional de Investigaciones Científicas y Técnicas
ID : PIP
Organisme : Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación
ID : 2021
Organisme : Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación
ID : 2021
Organisme : Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación
ID : 2021
Organisme : Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación
ID : 2021

Informations de copyright

© 2024. The Author(s).

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Auteurs

M A Dasilva (MA)

Instituto Multidisciplinario de Biología Vegetal (IMBIV), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Córdoba, Argentina.
Cátedra de Microbiología, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, X5000HUA, Argentina.

K F Crespo Andrada (KFC)

Cátedra de Microbiología, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, X5000HUA, Argentina.

M Maldonado Torales (MM)

Cátedra de Microbiología, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, X5000HUA, Argentina.

I Manrrique Hughes (IM)

Cátedra de Microbiología, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, X5000HUA, Argentina.

P Pez (P)

Cátedra de Microbiología, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, X5000HUA, Argentina.
Departamento de Ciencias Farmacéuticas, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.

J C García-Martínez (JC)

Facultad de Farmacia de Albacete, Centro Regional de Investigaciones Biomédicas, Universidad de Castilla-La Mancha, Ciudad Real, España.

María Gabriela Paraje (MG)

Instituto Multidisciplinario de Biología Vegetal (IMBIV), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Córdoba, Argentina. gabrielaparaje@gmail.com.
Cátedra de Microbiología, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, X5000HUA, Argentina. gabrielaparaje@gmail.com.

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