Metagenomic 16S rRNA analysis and predictive functional profiling revealed intrinsic organohalides respiration and bioremediation potential in mangrove sediment.
16S rRNA
Mangrove sediment
Metagenomic analysis
Organohalides bioremediation
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
22 May 2024
22 May 2024
Historique:
received:
21
11
2023
accepted:
03
04
2024
medline:
23
5
2024
pubmed:
23
5
2024
entrez:
22
5
2024
Statut:
epublish
Résumé
Mangrove sediment microbes are increasingly attracting scientific attention due to their demonstrated capacity for diverse bioremediation activities, encompassing a wide range of environmental contaminants. The microbial communities of five Avicennia marina mangrove sediment samples collected from Al Rayyis White Head, Red Sea (KSA), were characterized using Illumina amplicon sequencing of the 16S rRNA genes. Our study investigated the microbial composition and potential for organohalide bioremediation in five mangrove sediments from the Red Sea. While Proteobacteria dominated four microbiomes, Bacteroidetes dominated the fifth. Given the environmental concerns surrounding organohalides, their bioremediation is crucial. Encouragingly, we identified phylogenetically diverse organohalide-respiring bacteria (OHRB) across all samples, including Dehalogenimonas, Dehalococcoides, Anaeromyxobacter, Desulfuromonas, Geobacter, Desulfomonile, Desulfovibrio, Shewanella and Desulfitobacterium. These bacteria are known for their ability to dechlorinate organohalides through reductive dehalogenation. PICRUSt analysis further supported this potential, predicting the presence of functional biomarkers for organohalide respiration (OHR), including reductive dehalogenases targeting tetrachloroethene (PCE) and 3-chloro-4-hydroxyphenylacetate in most sediments. Enrichment cultures studies confirmed this prediction, demonstrating PCE dechlorination by the resident microbial community. PICRUSt also revealed a dominance of anaerobic metabolic processes, suggesting the microbiome's adaptation to the oxygen-limited environment of the sediments. This study provided insights into the bacterial community composition of five mangrove sediments from the Red Sea. Notably, diverse OHRB were detected across all samples, which possess the metabolic potential for organohalide bioremediation through reductive dehalogenation pathways. Furthermore, PICRUSt analysis predicted the presence of functional biomarkers for OHR in most sediments, suggesting potential intrinsic OHR activity by the enclosed microbial community.
Sections du résumé
BACKGROUND
BACKGROUND
Mangrove sediment microbes are increasingly attracting scientific attention due to their demonstrated capacity for diverse bioremediation activities, encompassing a wide range of environmental contaminants.
MATERIALS AND METHODS
METHODS
The microbial communities of five Avicennia marina mangrove sediment samples collected from Al Rayyis White Head, Red Sea (KSA), were characterized using Illumina amplicon sequencing of the 16S rRNA genes.
RESULTS
RESULTS
Our study investigated the microbial composition and potential for organohalide bioremediation in five mangrove sediments from the Red Sea. While Proteobacteria dominated four microbiomes, Bacteroidetes dominated the fifth. Given the environmental concerns surrounding organohalides, their bioremediation is crucial. Encouragingly, we identified phylogenetically diverse organohalide-respiring bacteria (OHRB) across all samples, including Dehalogenimonas, Dehalococcoides, Anaeromyxobacter, Desulfuromonas, Geobacter, Desulfomonile, Desulfovibrio, Shewanella and Desulfitobacterium. These bacteria are known for their ability to dechlorinate organohalides through reductive dehalogenation. PICRUSt analysis further supported this potential, predicting the presence of functional biomarkers for organohalide respiration (OHR), including reductive dehalogenases targeting tetrachloroethene (PCE) and 3-chloro-4-hydroxyphenylacetate in most sediments. Enrichment cultures studies confirmed this prediction, demonstrating PCE dechlorination by the resident microbial community. PICRUSt also revealed a dominance of anaerobic metabolic processes, suggesting the microbiome's adaptation to the oxygen-limited environment of the sediments.
CONCLUSION
CONCLUSIONS
This study provided insights into the bacterial community composition of five mangrove sediments from the Red Sea. Notably, diverse OHRB were detected across all samples, which possess the metabolic potential for organohalide bioremediation through reductive dehalogenation pathways. Furthermore, PICRUSt analysis predicted the presence of functional biomarkers for OHR in most sediments, suggesting potential intrinsic OHR activity by the enclosed microbial community.
Identifiants
pubmed: 38778276
doi: 10.1186/s12866-024-03291-8
pii: 10.1186/s12866-024-03291-8
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
DNA, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
176Informations de copyright
© 2024. The Author(s).
Références
Holguin G, Vazquez P, Bashan Y. The role of sediment microorganisms in the productivity, conservation, and rehabilitation of mangrove ecosystems: an overview. Biol Fertil Soils. 2001;33:265–78.
doi: 10.1007/s003740000319
Kathiresan K, Bingham BL. Biology of mangroves and mangrove ecosystems. Adv Mar Biol. 2001;40:81–251.
doi: 10.1016/S0065-2881(01)40003-4
Gomes NC, Cleary DF, Pires AC, Almeida A, Cunha A, Mendonça-Hagler LC, Smalla K. Assessing variation in bacterial composition between the rhizospheres of two mangrove tree species. Estuar Coast Shelf Sci. 2014;139:40–5.
doi: 10.1016/j.ecss.2013.12.022
Alongi DM. Carbon cycling and storage in mangrove forests. Ann Rev Mar Sci. 2014;6(1):195–219.
pubmed: 24405426
doi: 10.1146/annurev-marine-010213-135020
Liu M, Huang H, Bao S, Tong Y. Microbial community structure of soils in Bamenwan mangrove wetland. Sci Rep. 2019;9(1):1–11.
Kathiresan K, Selvam MM. Evaluation of beneficial bacteria from mangrove soil. Bot Mar. 2006;49:86–8. https://doi.org/10.1515/BOT.2006.011 .
doi: 10.1515/BOT.2006.011
Duke NC, Meynecke JO, Dittmann S, Ellison AM, Anger K, Berger U, et al. A world without mangroves. Sci. 2007;317(5834):41–2.
doi: 10.1126/science.317.5834.41b
Ramsay MA, Swannell RP, Shipton WA, Duke NC, Hill RT. Effect of bioremediation on the microbial community in oiled mangrove sediments. Mar Pollut Bull. 2000;41(7–12):413–9.
doi: 10.1016/S0025-326X(00)00137-5
Brito EM, Duran R, Guyoneaud R, Goñi-Urriza M, De Oteyza TG, Crapez MA, Wasserman JC. A case study of in situ oil contamination in a mangrove swamp (Rio De Janeiro, Brazil). Mar Pollut Bull. 2009;58(3):418–23.
pubmed: 19185324
doi: 10.1016/j.marpolbul.2008.12.008
Machado LF, de Assis Leite DC, da Costa Rachid CTC, Paes JE, Martins EF, Peixoto RS, Rosado AS. Tracking mangrove oil bioremediation approaches and bacterial diversity at different depths in an in situ mesocosms system. Front Microbiol. 2019;10: 2107.
pubmed: 31572322
pmcid: 6753392
doi: 10.3389/fmicb.2019.02107
Cabral L, Noronha MF, de Sousa STP, Lacerda-Júnior GV, Richter L, Fostier AH, de Oliveira VM. The metagenomic landscape of xenobiotics biodegradation in mangrove sediments. Ecotoxicol Environ Saf. 2019;179:232–40.
pubmed: 31051396
doi: 10.1016/j.ecoenv.2019.04.044
ATSDR, U. CERCLA priority list of hazardous substances. 2007. http://www.atsdr.cdc.gov/cercla/07list.html .
McCarty PL, Reinhard M. Biological and chemical transformations of halogenated aliphatic compounds in aquatic and terrestrial environments. In: Oremland RS, editor. The biogeochemistry of global change: radiative trace gases. New York: Chapman & Hall, Inc.; 1993. p. 839–52.
doi: 10.1007/978-1-4615-2812-8_45
Vogel TM, et al. Natural bioremediation of chlorinated solvents. In: Norris RD, editor., et al., Handbook of bioremediation. Boca Raton: Lewis Publishers; 1994. p. 201–25.
Jugder BE, Ertan H, Bohl S, Lee M, Marquis CP, Manefield M. Organohalide respiring bacteria and reductive dehalogenases: key tools in organohalide bioremediation. Front Microbiol. 2016;7:249.
pubmed: 26973626
pmcid: 4771760
doi: 10.3389/fmicb.2016.00249
Häggblom MM, Bossert ID. Halogenated organic compounds-a global perspective. In: Dehalogenation: microbial processes and environmental applications. Boston: Springer US; 2003. p. 3–29.
doi: 10.1007/b101852
Leys D, Adrian L, Smidt H. Organohalide respiration: microbes breathing chlorinated molecules. Philosophical Trans Royal Soc B Biol Sci. 2013;368(1616):20120316.
doi: 10.1098/rstb.2012.0316
Chapelle FH. Identifying redox conditions that favour the natural attenuation of chlorinated ethenes in contaminated ground-water systems. In: Symposium on natural attenuation of chlorinated organics in ground water. 1996. p. 17–20.
Gossett JM, Zinder SH. Microbiological aspects relevant to natural attenuation of chlorinated ethenes. In: Symposium on natural attenuation of chlorinated organics in ground water. 1997. p. 10–13.
El-Sayed WS, Al-Senani SR, Elbahloul Y. Diversity of dehalorespiring bacteria and selective enrichment of aryl halides-dechlorinating consortium from sedimentary environment near an oil refinery. J Taibah Univ Sci. 2018;12(6):711–22.
doi: 10.1080/16583655.2018.1495869
Zanaroli G, Balloi A, Negroni A, Daffonchio D, Young LY, Fava F. Characterization of the microbial community from the marine sediment of the Venice lagoon capable of reductive dechlorination of coplanar polychlorinated biphenyls (PCBs). J Haz Mat. 2010;178:417–26.
doi: 10.1016/j.jhazmat.2010.01.097
Kjellerup BV, Naff C, Edwards SJ, Ghosh U, Baker JE, Sowers KR. Effects of activated carbon on reductive dechlorination of PCBs by organohalide respiring bacteria indigenous to sediments. Water Res. 2014;52:1–10.
pubmed: 24440760
doi: 10.1016/j.watres.2013.12.030
Vandermeeren P, Herrmann S, Cichocka D, Busschaert P, Lievens B, Richnow HH, Springael D. Diversity of dechlorination pathways and organohalide respiring bacteria in chlorobenzene dechlorinating enrichment cultures originating from river sludge. Biodegradation. 2014;25(5):757–76.
pubmed: 25037978
doi: 10.1007/s10532-014-9697-y
El-Sayed WS. Characterization of a highly enriched microbial consortium reductively dechlorinating 2,3-dichlorophenol and 2,4,6-trichlorophenol and the corresponding cprA genes from river sediment. Pol J Microbiol. 2016;65(3):341.
pubmed: 29334051
doi: 10.5604/17331331.1215613
Zhu H, Wang Y, Wang X, Luan T, Tam NF. Distribution and accumulation of polybrominated diphenyl ethers (PBDEs) in Hong Kong mangrove sediments. Sci Total Environ. 2014;468:130–9.
pubmed: 24012900
doi: 10.1016/j.scitotenv.2013.08.021
Pan Y, Chen J, Zhou H, Cheung SG, Tam NF. Degradation of BDE-47 in mangrove sediments under alternating anaerobic-aerobic conditions. J Haz Mat. 2019;378:120709.
doi: 10.1016/j.jhazmat.2019.05.102
Robin SL, Marchand C. Polycyclic aromatic hydrocarbons (PAHs) in mangrove ecosystems: a review. Environ Pollut. 2022;311: 119959.
pubmed: 35977644
doi: 10.1016/j.envpol.2022.119959
Ghizelini AM, Mendonça-Hagler LCS, Macrae A. Microbial diversity in Brazilian mangrove sediments: a mini review. Braz J Microbiol. 2012;43:1242–54.
pubmed: 24031949
pmcid: 3769006
doi: 10.1590/S1517-83822012000400002
Meyer F, Paarmann D, D’Souza M, Olson R, Glass EM, Kubal M, et al. The metagenomics RAST server – a public resource for the automatic phylogenetic and functional analysis of metagenomes. BMC Bioinform. 2008;9:386.
doi: 10.1186/1471-2105-9-386
Cox MP, Peterson DA, Biggs PJ. SolexaQA: at-a-glance quality assessment of Illumina second-generation sequencing data. BMC Bioinform. 2010;11:485.
doi: 10.1186/1471-2105-11-485
Langmead B, Trapnell C, Pop M, Salzberg SL. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 2009;10: R25.
pubmed: 19261174
pmcid: 2690996
doi: 10.1186/gb-2009-10-3-r25
Pruesse E, Quast C, Knittel K, et al. SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Res. 2007;35(21):7188–96.
pubmed: 17947321
pmcid: 2175337
doi: 10.1093/nar/gkm864
Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol. 2017;67:1613.
pubmed: 28005526
pmcid: 5563544
doi: 10.1099/ijsem.0.001755
Rognes T, Flouri T, Nichols B, Quince C, Mahe F. VSEARCH: a versatile open source tool for metagenomics. PeerJ. 2016;4: e2584.
pubmed: 27781170
pmcid: 5075697
doi: 10.7717/peerj.2584
Edgar RC. Search and clustering orders of magnitude faster than BLAST. Bioinform. 2010;26(19):2460–1.
doi: 10.1093/bioinformatics/btq461
Fu L, Niu B, Zhu Z, Wu S, Li W. CD-HIT: accelerated for clustering the next-generation sequencing data. Bioinform. 2012;28(23):3150–2.
doi: 10.1093/bioinformatics/bts565
Langille MG, Zaneveld J, Caporaso JG, McDonald D, Knights D, Reyes JA, Huttenhower C. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat Biotechnol. 2013;31(9):814–21.
pubmed: 23975157
pmcid: 3819121
doi: 10.1038/nbt.2676
Kanehisa M, Goto S, Sato Y, Furumichi M, Tanabe M. KEGG for integration and interpretation of large-scale molecular data sets. Nucleic Acids res. 2012;40(D1):D109–114.
pubmed: 22080510
doi: 10.1093/nar/gkr988
Kim SS, Eun JW, Cho HJ, Song DS, Kim CW, Kim YS, Lee SW, Kim YK, Yang J, Choi J, et al. Microbiome as a potential diagnostic and predictive biomarker in severe alcoholic hepatitis. Aliment Pharmacol Ther. 2021;53(4):540–51.
pubmed: 33264437
doi: 10.1111/apt.16200
Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. 2018;35:1547–9.
pubmed: 29722887
pmcid: 5967553
doi: 10.1093/molbev/msy096
Widdel F, Kohring GW, Mayer F. Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids: III. Characterization of the filamentous gliding Desulfonema limicola gen. nov. sp. nov., and Desulfonema magnum sp. nov. Arch Microbiol. 1983;134:286–94.
doi: 10.1007/BF00407804
Ismaeil M, Yoshida N, Katayama A. Identification of multiple dehalogenase genes involved in tetrachloroethene-toethene dechlorination in a Dehalococcoides-dominated enrichment culture. BioMed Res Int. 2017;2017:9191086.
pubmed: 28894752
pmcid: 5574268
doi: 10.1155/2017/9191086
Atashgahi S, Lu Y, Smidt H. Overview of known organohalide-respiring bacteria – phylogenetic diversity and environmental distribution. In: Adrian L, Löffler FE, editors. Organohalide-respiring bacteria. Berlin: Springer Berlin Heidelberg; 2016. p. 63–105.
doi: 10.1007/978-3-662-49875-0_5
Türkowsky D, Jehmlich N, Diekert G, Adrian L, Von Bergen M, Goris T. An integrative overview of genomic, transcriptomic and proteomic analyses in organohalide respiration research. FEMS Microbiol Ecol. 2018;94(3):fiy013.
doi: 10.1093/femsec/fiy013
Villemur R, Lanthier M, Beaudet R, Lépine F. The Desulfitobacterium genus. FEMS Microbiol rev. 2006;30(5):706–33.
pubmed: 16911041
doi: 10.1111/j.1574-6976.2006.00029.x
Cole JR, Cascarelli AL, Mohn WW, Tiedje JM. Isolation and characterization of a novel bacterium growing via reductive dehalogenation of 2-chlorophenol. Appl Environ Microbiol. 1994;60(10):3536–42.
pubmed: 7527200
pmcid: 201851
doi: 10.1128/aem.60.10.3536-3542.1994
Sanford RA, Cole JR, Tiedje JM. Characterization and description of Anaeromyxobacter dehalogenans gen. nov., sp. nov., an aryl-halorespiring facultative anaerobic myxobacterium. Appl Environ Microbiol. 2002;68(2):893–900.
pubmed: 11823233
pmcid: 126698
doi: 10.1128/AEM.68.2.893-900.2002
Krumholz LR. Desulfuromonas chloroethenica sp. nov. uses tetrachloroethylene andtrichloroethylene as electron acceptors. Int J Syst Bacteriol. 1997;47(4):1262–3.
doi: 10.1099/00207713-47-4-1262
Fredrickson JK, Romine MF, Beliaev AS, Auchtung JM, Driscoll ME, Gardner TS, et al. Towards environmental systems biology of Shewanella. Nat Rev Microbiol. 2008;6:592–603.
pubmed: 18604222
doi: 10.1038/nrmicro1947
Sung Y, Fletcher KE, Ritalahti KM, Apkarian RP, Ramos-Hernández N, Sanford RA, Löffler FE. Geobacter lovleyi sp. nov. strain SZ, a novel metal-reducing and tetrachloroethene-dechlorinating bacterium. Appl Environ Microbiol. 2006;72(4):2775–82.
pubmed: 16597982
pmcid: 1448980
doi: 10.1128/AEM.72.4.2775-2782.2006
Ding C, He J. Molecular techniques in the biotechnological fight against halogenated compounds in anoxic environments. Microb Biotechnol. 2012;5(3):347–67.
pubmed: 22070763
pmcid: 3821678
doi: 10.1111/j.1751-7915.2011.00313.x
Basak P, Pramanik A, Sengupta S, Nag S, Bhattacharyya A, Roy D, Bhattacharyya M. Bacterial diversity assessment of pristine mangrove microbial community from Dhulibhashani, Sundarbans using 16S rRNA gene tag sequencing. Genom Data. 2016;7:76–8.
pubmed: 26981367
doi: 10.1016/j.gdata.2015.11.030
Mendes LW, Tsai SM. Variations of bacterial community structure and composition in mangrove sediment at different depths in Southeastern Brazil. Diversity. 2014;6:827–43.
doi: 10.3390/d6040827
Wu P, Xiong X, Xu Z, Lu C, Cheng H, Lyu X, Lou Q. Bacterial communities in the rhizospheres of three mangrove tree species from Beilun Estuary, China. PLoS One. 2016;11(10): e0164082.
pubmed: 27695084
pmcid: 5047532
doi: 10.1371/journal.pone.0164082
Zhao H, Yan B, Mo S, Nie S, Li Q, Ou Q, Wu B, Jiang G, Tang J, Li N, Jiang C. Carbohydrate metabolism genes dominant in a subtropical marine mangrove ecosystem revealed by metagenomics analysis. J Microbiol. 2019;57:575–86.
pubmed: 31073898
doi: 10.1007/s12275-019-8679-5
Alzubaidy H, Essack M, Malas TB, Bokhari A, Motwalli O, Kamanu FK, Archer JA. Rhizosphere microbiome metagenomics of gray mangroves (Avicennia marina) in the Red Sea. Gene. 2016;576(2):626–36.
pubmed: 26475934
doi: 10.1016/j.gene.2015.10.032
Sanka Loganathachetti D, Sadaiappan B, Poosakkannu A, Muthuraman S. Pyrosequencing-based seasonal observation of prokaryotic diversity in pneumatophore-associated soil of Avicennia marina. Curr Microbiol. 2016;72:68–74.
pubmed: 26446550
doi: 10.1007/s00284-015-0920-9
Fennell DE, Nijenhuis I, Wilson SF, Zinder SH, Häggblom MM. Dehalococcoides ethenogenes strain 195 reductively dechlorinates diverse chlorinated aromatic pollutants. Environ sci Technol. 2004;38(7):2075–81.
pubmed: 15112809
doi: 10.1021/es034989b
Wang S, Chng KR, Wilm A, Zhao S, Yang KL, Nagarajan N, He J. Genomic characterization of three unique dehalococcoides that respire on persistent polychlorinated biphenyls. Proc Natl Acad Sci. 2014;111(33):12103–8.
pubmed: 25028492
pmcid: 4142991
doi: 10.1073/pnas.1404845111
Yang Y, Higgins SA, Yan J, Şimşir B, Chourey K, Iyer R, Löffler FE. Grape pomace compost harbors organohalide-respiring Dehalogenimonas species with novel reductive dehalogenase genes. ISME J. 2017;11(12):2767–80.
pubmed: 28809851
pmcid: 5702733
doi: 10.1038/ismej.2017.127
Pan Y, Chen J, Zhou H, Farzana S, Tam NF. Vertical distribution of dehalogenating bacteria in mangrove sediment and their potential to remove polybrominated diphenyl ether contamination. Mar Pollut bull. 2017;124(2):1055–62.
pubmed: 28034497
doi: 10.1016/j.marpolbul.2016.12.030
Liang Y, Lu Q, Liang Z, Liu X, Fang W, Liang D, Wang S. Substrate-dependent competition and cooperation relationships between Geobacter and Dehalococcoides for their organohalide respiration. ISME Commun. 2021;1(1):23.
pubmed: 37938613
pmcid: 9723705
doi: 10.1038/s43705-021-00025-z
Fernandes SO, Bonin PC, Michotey VD, Garcia N, LokaBharathi PA. Nitrogen-limited mangrove ecosystems conserve N through dissimilatory nitrate reduction to ammonium. Sci Rep. 2012;2: 419.
pubmed: 22639727
pmcid: 3358729
doi: 10.1038/srep00419
Cao WZ, Yang JX, Li Y, Liu BL, Wang FF, Chang CT. Dissimilatory nitrate reduction to ammonium conserves nitrogen in anthropogenically affected subtropical mangrove sediments in Southeast China. Mar Pollut Bull. 2016;110:155–61.
pubmed: 27368926
doi: 10.1016/j.marpolbul.2016.06.068
Li CH, Wong YS, Tam NFY. Anaerobic biodegradation of polycyclic aromatic hydrocarbons with amendment of iron (III) in mangrove sediment slurry. Bioresour Technol. 2010;101:8083–92.
pubmed: 20594830
doi: 10.1016/j.biortech.2010.06.005
Li CH, Zhou HW, Wong YS, Tam NFY. Vertical distribution and anaerobic biodegradation of polycyclic aromatic hydrocarbons in mangrove sediments in Hong Kong, South China. Sci Total Environ. 2009;407:5772–9.
pubmed: 19683792
doi: 10.1016/j.scitotenv.2009.07.034
Andrade LL, Leite DC, Ferreira EM, Ferreira LQ, Paula GR, Maguire MJ, Hubert CR, Peixoto RS, Domingues RM, Rosado AS. Microbial diversity and anaerobic hydrocarbon degradation potential in an oil-contaminated mangrove sediment. BMC Microbiol. 2012;12: 186.
pubmed: 22935169
pmcid: 3579730
doi: 10.1186/1471-2180-12-186
Lyu Z, Shao N, Akinyemi T, Whitman WB. Methanogenesis. Curr Biol. 2018;28:R727–32.
pubmed: 29990451
doi: 10.1016/j.cub.2018.05.021
Wuebbles DJ, Hayhoe K. Atmospheric methane and global change. Earth Sci Rev. 2002;57:177–210.
doi: 10.1016/S0012-8252(01)00062-9
Allen DE, Dalal RC, Rennenberg H, Meyer RL, Reeves S, Schmidt S. Spatial and temporal variation of nitrous oxide and methane flux between subtropical mangrove sediments and the atmosphere. Soil Biol Biochem. 2007;39:622–31.
doi: 10.1016/j.soilbio.2006.09.013
Chan OC, Wolf M, Hepperle D, Casper P. Methanogenic archaeal community in the sediment of an artificially partitioned acidic bog lake. FEMS Microbiol Ecol. 2002;42:119–29.
pubmed: 19709271
doi: 10.1111/j.1574-6941.2002.tb01001.x
Futagami T, Goto M, Furukawa K. Biochemical and genetic bases of dehalorespiration. Chem Rec. 2008;8(1):1–12.
pubmed: 18302277
doi: 10.1002/tcr.20134
Maymó-Gatell X, Nijenhuis I, Zinder SH. Reductive dechlorination of cis-1, 2-dichloroethene and vinyl chloride by “Dehalococcoides ethenogenes”. Environ Sci Technol. 2001;35(3):516–21.
pubmed: 11351722
doi: 10.1021/es001285i
Wang YF, Zhu HW, Wang Y, Zhang XL, Tam NFY. Diversity and dynamics of microbial community structure in different mangrove, marine and freshwater sediments during anaerobic debromination of PBDEs. Front Microbiol. 2018;9:952.
pubmed: 29867858
pmcid: 5962692
doi: 10.3389/fmicb.2018.00952
Chen J, Wang C, Pan Y, Farzana SS, Tam NFY. Biochar accelerates microbial reductive debromination of 2, 2′, 4, 4′-tetrabromodiphenyl ether (BDE-47) in anaerobic mangrove sediments. J Hazard Mater. 2018;341:177–86.
pubmed: 28777963
doi: 10.1016/j.jhazmat.2017.07.063