Blocking of amino acid transporter OsAAP7 promoted tillering and yield by determining basic and neutral amino acids accumulation in rice.
Amino acid
Rice
Tillering
Transporter
Yield
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
23 May 2024
23 May 2024
Historique:
received:
16
01
2024
accepted:
16
05
2024
medline:
24
5
2024
pubmed:
24
5
2024
entrez:
23
5
2024
Statut:
epublish
Résumé
Amino acids are not only the main form of N in rice, but also are vital for its growth and development. These processes are facilitated by amino acid transporters within the plant. Despite their significance, only a few AAP amino acid transporters have been reported. In this study, we observed that there were differences in the expression of amino acid transporter OsAAP7 among 521 wild cultivated rice varieties, and it directly negatively correlated with tillering and grain yield per plant. We revealed that OsAAP7 protein was localized to the endoplasmic reticulum and had absorption and transport affinity for amino acids such as phenylalanine (Phe), lysine (Lys), leucine (Leu), and arginine (Arg) using subcellular localization, yeast substrate testing, fluorescent amino acid uptake, and amino acid content determination. Further hydroponic studies showed that exogenous application of amino acids Phe, Lys and Arg inhibited the growth of axillary buds in the overexpression lines, and promoted the elongation of axillary buds in the mutant lines. Finally, RNA-seq analysis showed that the expression patterns of genes related to nitrogen, auxin and cytokinin pathways were changed in axillary buds of OsAAP7 transgenic plants. This study revealed the gene function of OsAAP7, and found that blocking of amino acid transporter OsAAP7 with CRISPR/Cas9 technology promoted tillering and yield by determining basic and neutral amino acids accumulation in rice.
Sections du résumé
BACKGROUND
BACKGROUND
Amino acids are not only the main form of N in rice, but also are vital for its growth and development. These processes are facilitated by amino acid transporters within the plant. Despite their significance, only a few AAP amino acid transporters have been reported.
RESULTS
RESULTS
In this study, we observed that there were differences in the expression of amino acid transporter OsAAP7 among 521 wild cultivated rice varieties, and it directly negatively correlated with tillering and grain yield per plant. We revealed that OsAAP7 protein was localized to the endoplasmic reticulum and had absorption and transport affinity for amino acids such as phenylalanine (Phe), lysine (Lys), leucine (Leu), and arginine (Arg) using subcellular localization, yeast substrate testing, fluorescent amino acid uptake, and amino acid content determination. Further hydroponic studies showed that exogenous application of amino acids Phe, Lys and Arg inhibited the growth of axillary buds in the overexpression lines, and promoted the elongation of axillary buds in the mutant lines. Finally, RNA-seq analysis showed that the expression patterns of genes related to nitrogen, auxin and cytokinin pathways were changed in axillary buds of OsAAP7 transgenic plants.
CONCLUSIONS
CONCLUSIONS
This study revealed the gene function of OsAAP7, and found that blocking of amino acid transporter OsAAP7 with CRISPR/Cas9 technology promoted tillering and yield by determining basic and neutral amino acids accumulation in rice.
Identifiants
pubmed: 38783192
doi: 10.1186/s12870-024-05159-5
pii: 10.1186/s12870-024-05159-5
doi:
Substances chimiques
Plant Proteins
0
Amino Acid Transport Systems
0
Amino Acids, Neutral
0
Amino Acids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
447Subventions
Organisme : National Natural Science Foundation of China
ID : 32060064/32260498
Organisme : Guizhou Provincial Excellent Young Talents Project of Science and Technology
ID : qiankehepingtairencai-YQK (2023) 002
Organisme : Guizhou Provincial Science and Technology Projects
ID : qiankehechengguo(2024) General 116; qiankehejichu-ZK (2021) General 128; qiankehejichu-ZK (2022) Key 008
Organisme : Key Laboratory of Molecular Breeding for Grain and Oil Crops in Guizhou Province
ID : Qiankehezhongyindi (2023) 008
Organisme : Key Laboratory of Functional Agriculture of Guizhou Provincial Department of Education
ID : Qianjiaoji (2023)007
Organisme : Qiandongnan Science and Technology Support Project
ID : Qiandongnan Kehe Support (2023)06
Informations de copyright
© 2024. The Author(s).
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