Contractile injection systems facilitate sporogenic differentiation of Streptomyces davawensis through the action of a phage tapemeasure protein-related effector.

Streptomyces / virology Bacteriophages / genetics Spores, Bacterial / metabolism Bacterial Proteins / metabolism Phylogeny Viral Proteins / metabolism Viral Tail Proteins / metabolism

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
24 May 2024
Historique:
received: 17 11 2023
accepted: 13 05 2024
medline: 25 5 2024
pubmed: 25 5 2024
entrez: 24 5 2024
Statut: epublish

Résumé

Contractile injection systems (CISs) are prokaryotic phage tail-like nanostructures loading effector proteins that mediate various biological processes. Although CIS functions have been diversified through evolution and hold the great potential as protein delivery systems, the functional characterisation of CISs and their effectors is currently limited to a few CIS lineages. Here, we show that the CISs of Streptomyces davawensis belong to a unique group of bacterial CISs distributed across distant phyla and facilitate sporogenic differentiation of this bacterium. CIS loss results in decreases in extracellular DNA release, biomass accumulation, and spore formation in S. davawensis. CISs load an effector, which is a remote homolog of phage tapemeasure proteins, and its C-terminal domain has endonuclease activity responsible for the CIS-associated phenotypes. Our findings illustrate that CISs can contribute to the reproduction of bacteria through the action of the effector and suggest an evolutionary link between CIS effectors and viral cargos.

Identifiants

DOI: 10.1038/s41467-024-48834-9 PMID: 38789435
pubmed: 38789435
doi: 10.1038/s41467-024-48834-9
pii: 10.1038/s41467-024-48834-9
doi:

Substances chimiques

Bacterial Proteins 0
Viral Proteins 0
Viral Tail Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

4442

Subventions

Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 19K15726
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 23K13863
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 23K26811
Organisme : MEXT | Japan Science and Technology Agency (JST)
ID : JPMJMI21G8
Organisme : MEXT | Japan Science and Technology Agency (JST)
ID : JPMJGX23B2

Informations de copyright

© 2024. The Author(s).

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Auteurs

Toshiki Nagakubo (T)

Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan. nagakubo.toshiki.gp@u.tsukuba.ac.jp.
Microbiology Research Center for Sustainability (MiCS), University of Tsukuba, Tsukuba, Japan. nagakubo.toshiki.gp@u.tsukuba.ac.jp.
ORCID: 0000-0003-0661-0304

Tatsuya Nishiyama (T)

Life Science Research Center, College of Bioresource Sciences, Nihon University, Chiyoda, Japan.

Tatsuya Yamamoto (T)

Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan.

Nobuhiko Nomura (N)

Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan.
Microbiology Research Center for Sustainability (MiCS), University of Tsukuba, Tsukuba, Japan.
Life Science Center for Survival Dynamics, University of Tsukuba, Tsukuba, Japan.

Masanori Toyofuku (M)

Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan. toyofuku.masanori.gf@u.tsukuba.ac.jp.
Microbiology Research Center for Sustainability (MiCS), University of Tsukuba, Tsukuba, Japan. toyofuku.masanori.gf@u.tsukuba.ac.jp.
ORCID: 0000-0003-4627-9230

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Streptomycetaceae › Streptomyces › Contractile injection systems facilitate...
questionsmedicales.fr › Virus › Bactériophages › Contractile injection systems facilitate...
questionsmedicales.fr › Stades d'organismes › Spores › Spores bactériens › Contractile injection systems facilitate...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Contractile injection systems facilitate...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Contractile injection systems facilitate...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Contractile injection systems facilitate...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales structurales › Protéines virales queue › Contractile injection systems facilitate...