Rhytidome- and cork-type barks of holm oak, cork oak and their hybrids highlight processes leading to cork formation.


Journal

BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807

Informations de publication

Date de publication:
03 Jun 2024
Historique:
received: 02 08 2023
accepted: 23 05 2024
medline: 3 6 2024
pubmed: 3 6 2024
entrez: 2 6 2024
Statut: epublish

Résumé

The periderm is basic for land plants due to its protective role during radial growth, which is achieved by the polymers deposited in the cell walls. In most trees, like holm oak, the first periderm is frequently replaced by subsequent internal periderms yielding a heterogeneous outer bark made of a mixture of periderms and phloem tissues, known as rhytidome. Exceptionally, cork oak forms a persistent or long-lived periderm which results in a homogeneous outer bark of thick phellem cell layers known as cork. Cork oak and holm oak distribution ranges overlap to a great extent, and they often share stands, where they can hybridize and produce offspring showing a rhytidome-type bark. Here we use the outer bark of cork oak, holm oak, and their natural hybrids to analyse the chemical composition, the anatomy and the transcriptome, and further understand the mechanisms underlying periderm development. We also include a unique natural hybrid individual corresponding to a backcross with cork oak that, interestingly, shows a cork-type bark. The inclusion of hybrid samples showing rhytidome-type and cork-type barks is valuable to approach cork and rhytidome development, allowing an accurate identification of candidate genes and processes. The present study underscores that abiotic stress and cell death are enhanced in rhytidome-type barks whereas lipid metabolism and cell cycle are enriched in cork-type barks. Development-related DEGs showing the highest expression, highlight cell division, cell expansion, and cell differentiation as key processes leading to cork or rhytidome-type barks. Transcriptome results, in agreement with anatomical and chemical analyses, show that rhytidome and cork-type barks are active in periderm development, and suberin and lignin deposition. Development and cell wall-related DEGs suggest that cell division and expansion are upregulated in cork-type barks whereas cell differentiation is enhanced in rhytidome-type barks.

Sections du résumé

BACKGROUND BACKGROUND
The periderm is basic for land plants due to its protective role during radial growth, which is achieved by the polymers deposited in the cell walls. In most trees, like holm oak, the first periderm is frequently replaced by subsequent internal periderms yielding a heterogeneous outer bark made of a mixture of periderms and phloem tissues, known as rhytidome. Exceptionally, cork oak forms a persistent or long-lived periderm which results in a homogeneous outer bark of thick phellem cell layers known as cork. Cork oak and holm oak distribution ranges overlap to a great extent, and they often share stands, where they can hybridize and produce offspring showing a rhytidome-type bark.
RESULTS RESULTS
Here we use the outer bark of cork oak, holm oak, and their natural hybrids to analyse the chemical composition, the anatomy and the transcriptome, and further understand the mechanisms underlying periderm development. We also include a unique natural hybrid individual corresponding to a backcross with cork oak that, interestingly, shows a cork-type bark. The inclusion of hybrid samples showing rhytidome-type and cork-type barks is valuable to approach cork and rhytidome development, allowing an accurate identification of candidate genes and processes. The present study underscores that abiotic stress and cell death are enhanced in rhytidome-type barks whereas lipid metabolism and cell cycle are enriched in cork-type barks. Development-related DEGs showing the highest expression, highlight cell division, cell expansion, and cell differentiation as key processes leading to cork or rhytidome-type barks.
CONCLUSION CONCLUSIONS
Transcriptome results, in agreement with anatomical and chemical analyses, show that rhytidome and cork-type barks are active in periderm development, and suberin and lignin deposition. Development and cell wall-related DEGs suggest that cell division and expansion are upregulated in cork-type barks whereas cell differentiation is enhanced in rhytidome-type barks.

Identifiants

pubmed: 38825683
doi: 10.1186/s12870-024-05192-4
pii: 10.1186/s12870-024-05192-4
doi:

Substances chimiques

suberin 8072-95-5
Lipids 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

488

Subventions

Organisme : Agencia Estatal de Investigación
ID : AGL2015-67495-C2-1-R (MINECO/FEDER,UE)
Organisme : Agencia Estatal de Investigación
ID : AGL2015-67495-C2-2-R (MINECO/FEDER,UE)
Organisme : Agencia Estatal de Investigación
ID : AGL2015-67495-C2-1-R (MINECO/FEDER,UE)
Organisme : Agencia Estatal de Investigación
ID : PID2019-110330GB-C21 (MCI/ AEI)
Organisme : Agencia Estatal de Investigación
ID : AGL2015-67495-C2-1-R (MINECO/FEDER,UE)
Organisme : Agencia Estatal de Investigación
ID : AGL2015-67495-C2-2-R (MINECO/FEDER,UE)
Organisme : Agencia Estatal de Investigación
ID : AGL2015-67495-C2-1-R (MINECO/FEDER,UE)
Organisme : Agencia Estatal de Investigación
ID : AGL2015-67495-C2-1-R (MINECO/FEDER,UE)
Organisme : Ministerio de Educación y Formación Profesional
ID : beca colaboración 2021_2022

Informations de copyright

© 2024. The Author(s).

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Auteurs

Iker Armendariz (I)

Laboratori del suro, Departament de Biologia, Facultat de Ciències, Universitat de Girona, Carrer Maria Aurèlia Campmany 40, Girona, 17003, Spain.

Unai López de Heredia (U)

Departamento de Sistemas y Recursos Naturales. ETSI Montes, Forestal y del Medio Natural, Universidad Politécnica de Madrid, José Antonio Novais 10, Madrid, 28040, Spain.

Marçal Soler (M)

Laboratori del suro, Departament de Biologia, Facultat de Ciències, Universitat de Girona, Carrer Maria Aurèlia Campmany 40, Girona, 17003, Spain.

Adrià Puigdemont (A)

Laboratori del suro, Departament de Biologia, Facultat de Ciències, Universitat de Girona, Carrer Maria Aurèlia Campmany 40, Girona, 17003, Spain.

Maria Mercè Ruiz (MM)

Institut Català del Suro. Carrer Miquel Vincke i Meyer 13, Palafrugell, 17200, Spain.

Patricia Jové (P)

Institut Català del Suro. Carrer Miquel Vincke i Meyer 13, Palafrugell, 17200, Spain.

Álvaro Soto (Á)

Departamento de Sistemas y Recursos Naturales. ETSI Montes, Forestal y del Medio Natural, Universidad Politécnica de Madrid, José Antonio Novais 10, Madrid, 28040, Spain.

Olga Serra (O)

Laboratori del suro, Departament de Biologia, Facultat de Ciències, Universitat de Girona, Carrer Maria Aurèlia Campmany 40, Girona, 17003, Spain.

Mercè Figueras (M)

Laboratori del suro, Departament de Biologia, Facultat de Ciències, Universitat de Girona, Carrer Maria Aurèlia Campmany 40, Girona, 17003, Spain. merce.figueras@udg.edu.

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