The essential role of aggregation for the emulsifying ability of a fungal CYS-rich protein.
Aggregation
Bioemulsifier
CFEM domain
Fungal proteins
Plastic-degrading fungi
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
03 Jun 2024
03 Jun 2024
Historique:
received:
08
02
2024
accepted:
10
05
2024
revised:
29
04
2024
medline:
3
6
2024
pubmed:
3
6
2024
entrez:
3
6
2024
Statut:
epublish
Résumé
Biosurfactants are in demand by the global market as natural commodities suitable for incorporation into commercial products or utilization in environmental applications. Fungi are promising producers of these molecules and have garnered interest also for their metabolic capabilities in efficiently utilizing recalcitrant and complex substrates, like hydrocarbons, plastic, etc. Within this framework, biosurfactants produced by two Fusarium solani fungal strains, isolated from plastic waste-contaminated landfill soils, were analyzed. Mycelia of these fungi were grown in the presence of 5% olive oil to drive biosurfactant production. The characterization of the emulsifying and surfactant capacity of these extracts highlighted that two different components are involved. A protein was purified and identified as a CFEM (common in fungal extracellular membrane) containing domain, revealing a good propensity to stabilize emulsions only in its aggregate form. On the other hand, an unidentified cationic smaller molecule exhibits the ability to reduce surface tension. Based on the 3D structural model of the protein, a plausible mechanism for the formation of very stable aggregates, endowed with the emulsifying ability, is proposed. KEY POINTS: • Two Fusarium solani strains are analyzed for their surfactant production. • A cationic surfactant is produced, exhibiting the ability to remarkably reduce surface tension. • An identified protein reveals a good propensity to stabilize emulsions only in its aggregate form.
Identifiants
pubmed: 38829381
doi: 10.1007/s00253-024-13182-7
pii: 10.1007/s00253-024-13182-7
doi:
Substances chimiques
Fungal Proteins
0
Surface-Active Agents
0
Emulsifying Agents
0
Emulsions
0
Cysteine
K848JZ4886
Olive Oil
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
358Subventions
Organisme : European Union Next-Generation EU
ID : PE00000004
Informations de copyright
© 2024. The Author(s).
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