Fate of telomere entanglements is dictated by the timing of anaphase midregion nuclear envelope breakdown.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
03 Jun 2024
03 Jun 2024
Historique:
received:
21
01
2024
accepted:
29
04
2024
medline:
4
6
2024
pubmed:
4
6
2024
entrez:
3
6
2024
Statut:
epublish
Résumé
Persisting replication intermediates can confer mitotic catastrophe. Loss of the fission yeast telomere protein Taz1 (ortholog of mammalian TRF1/TRF2) causes telomeric replication fork (RF) stalling and consequently, telomere entanglements that stretch between segregating mitotic chromosomes. At ≤20 °C, these entanglements fail to resolve, resulting in lethality. Rif1, a conserved DNA replication/repair protein, hinders the resolution of telomere entanglements without affecting their formation. At mitosis, local nuclear envelope (NE) breakdown occurs in the cell's midregion. Here we demonstrate that entanglement resolution occurs in the cytoplasm following this NE breakdown. However, in response to taz1Δ telomeric entanglements, Rif1 delays midregion NE breakdown at ≤20 °C, in turn disfavoring entanglement resolution. Moreover, Rif1 overexpression in an otherwise wild-type setting causes cold-specific NE defects and lethality, which are rescued by membrane fluidization. Hence, NE properties confer the cold-specificity of taz1Δ lethality, which stems from postponement of NE breakdown. We propose that such postponement promotes clearance of simple stalled RFs, but resolution of complex entanglements (involving strand invasion between nonsister telomeres) requires rapid exposure to the cytoplasm.
Identifiants
pubmed: 38830842
doi: 10.1038/s41467-024-48382-2
pii: 10.1038/s41467-024-48382-2
doi:
Substances chimiques
Schizosaccharomyces pombe Proteins
0
Telomere-Binding Proteins
0
taz1 protein, S pombe
0
Rif1 protein, S pombe
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4707Subventions
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : GM145820-01
Informations de copyright
© 2024. The Author(s).
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