Overexpression of PavHIPP16 from Prunus avium enhances cold stress tolerance in transgenic tobacco.
Genetic transformation
HIPP
Low-temperature stress
Protein interaction
Sweet cherry
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
12 Jun 2024
12 Jun 2024
Historique:
received:
15
01
2024
accepted:
07
06
2024
medline:
12
6
2024
pubmed:
12
6
2024
entrez:
11
6
2024
Statut:
epublish
Résumé
The heavy metal-associated isoprenylated plant protein (HIPP) is an important regulatory element in response to abiotic stresses, especially playing a key role in low-temperature response. This study investigated the potential function of PavHIPP16 up-regulated in sweet cherry under cold stress by heterologous overexpression in tobacco. The results showed that the overexpression (OE) lines' growth state was better than wild type (WT), and the germination rate, root length, and fresh weight of OE lines were significantly higher than those of WT. In addition, the relative conductivity and malondialdehyde (MDA) content of the OE of tobacco under low-temperature treatment were substantially lower than those of WT. In contrast, peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) activities, hydrogen peroxide (H This study provides genetic resources for analyzing the biological functions of PavHIPPs, which is important for elucidating the mechanisms of cold resistance in sweet cherry.
Sections du résumé
BACKGROUND
BACKGROUND
The heavy metal-associated isoprenylated plant protein (HIPP) is an important regulatory element in response to abiotic stresses, especially playing a key role in low-temperature response.
RESULTS
RESULTS
This study investigated the potential function of PavHIPP16 up-regulated in sweet cherry under cold stress by heterologous overexpression in tobacco. The results showed that the overexpression (OE) lines' growth state was better than wild type (WT), and the germination rate, root length, and fresh weight of OE lines were significantly higher than those of WT. In addition, the relative conductivity and malondialdehyde (MDA) content of the OE of tobacco under low-temperature treatment were substantially lower than those of WT. In contrast, peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) activities, hydrogen peroxide (H
CONCLUSIONS
CONCLUSIONS
This study provides genetic resources for analyzing the biological functions of PavHIPPs, which is important for elucidating the mechanisms of cold resistance in sweet cherry.
Identifiants
pubmed: 38862890
doi: 10.1186/s12870-024-05267-2
pii: 10.1186/s12870-024-05267-2
doi:
Substances chimiques
Plant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
536Subventions
Organisme : the National Natural Science Foundation of China
ID : Grant No. 32160700
Organisme : he Guizhou Provincial Science and Technology Projects of China
ID : Grant No. YQK[2023]008
Organisme : the National Guidance of Local Science and Technology Development Fund of China
ID : Grant No. [2023]009
Organisme : the Guizhou Provincial Science and Technology Projects of China
ID : Grant No. [2021] Yiban231
Informations de copyright
© 2024. The Author(s).
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