Controlling the expression of heterologous genes in Bdellovibrio bacteriovorus using synthetic biology strategies.
Journal
Microbial biotechnology
ISSN: 1751-7915
Titre abrégé: Microb Biotechnol
Pays: United States
ID NLM: 101316335
Informations de publication
Date de publication:
Jun 2024
Jun 2024
Historique:
received:
23
03
2024
accepted:
14
06
2024
medline:
27
6
2024
pubmed:
27
6
2024
entrez:
27
6
2024
Statut:
ppublish
Résumé
Bdellovibrio bacteriovorus HD100 is an obligate predatory bacterium that preys upon Gram-negative bacteria. It has been proposed to be applied as a "living antibiotic" in several fields such as agriculture or even medicine, since it is able to prey upon bacterial pathogens. Its interesting lifestyle makes this bacterium very attractive as a microbial chassis for co-culture systems including two partners. A limitation to this goal is the scarcity of suitable synthetic biology tools for predator domestication. To fill this gap, we have firstly adapted the hierarchical assembly cloning technique Golden Standard (GS) to make it compatible with B. bacteriovorus HD100. The chromosomal integration of the Tn7 transposon's mobile element, in conjunction with the application of the GS technique, has allowed the systematic characterization of a repertoire of constitutive and inducible promoters, facilitating the control of the expression of heterologous genes in this bacterium. PJ
Identifiants
pubmed: 38934530
doi: 10.1111/1751-7915.14517
doi:
Substances chimiques
DNA Transposable Elements
0
Recombinant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14517Subventions
Organisme : Horizon 2020 Framework Programme
ID : 76099
Organisme : HORIZON EUROPE Framework Programme
ID : 101081776
Organisme : Agencia Estatal de Investigación
ID : BIO2017-8344-8-R
Organisme : Agencia Estatal de Investigación
ID : PID2020-112766RB-C21
Organisme : Comunidad de Madrid
ID : P2018/NMT4389
Organisme : Spanish Ministry of Science, Innovation and Universities
ID : FPU17/03978
Informations de copyright
© 2024 The Author(s). Microbial Biotechnology published by John Wiley & Sons Ltd.
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