One-pot electrochemical detection of foodborne pathogen based on in situ nucleic acid amplification and wash-free assay.
Electrochemical biosensor
Food pathogens detection
Loop-mediated isothermal amplification
Methylene blue
Screen printed carbon electrode
Journal
Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782
Informations de publication
Date de publication:
29 Jun 2024
29 Jun 2024
Historique:
received:
03
04
2024
accepted:
10
06
2024
medline:
2
7
2024
pubmed:
2
7
2024
entrez:
1
7
2024
Statut:
epublish
Résumé
A signal amplification electrochemical biosensor chip was developed to integrate loop-mediated isothermal amplification (LAMP) based on in situ nucleic acid amplification and methyl blue (MB) serving as the hybridization redox indicator for sensitive and selective foodborne pathogen detection without a washing step. The electrochemical biosensor chip was designed by a screen-printed carbon electrode modified with gold nanoparticles (Au NPs) and covered with polydimethylsiloxane membrane to form a microcell. The primers of the target were immobilized on the Au NPs by covalent attachment for in situ amplification. The electroactive MB was used as the electrochemical signal reporter and embedded into the double-stranded DNA (dsDNA) amplicons generated by LAMP. Differential pulse voltammetry was introduced to survey the dsDNA hybridization with MB, which differentiates the specifically electrode-unbound and -bound labels without a washing step. Pyrene as the back-filling agent can further improve response signaling by reducing non-specific adsorption. This method is operationally simple, specific, and effective. The biosensor showed a detection linear range of 10
Identifiants
pubmed: 38951263
doi: 10.1007/s00604-024-06500-3
pii: 10.1007/s00604-024-06500-3
doi:
Substances chimiques
Gold
7440-57-5
DNA, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
431Subventions
Organisme : Science and Technology Benefiting the People Demonstration Project of Qingdao
ID : 22-2-7-smjk-2-nsh
Organisme : Key Project of Shandong Province Natural Science Foundation
ID : ZR2020KH030
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
Références
Yu Z, Qiu C, Huang L, Gao Y, Tang D (2023) Microelectromechanical microsystems-supported photothermal immunoassay for point-of-care testing of aflatoxin B1 in foodstuff. Anal Chem 95:4212–4219. https://doi.org/10.1021/acs.analchem.2c05617
doi: 10.1021/acs.analchem.2c05617
pubmed: 36780374
Aung KT, Chen HJ, Chau ML, Yap G, Lim XF, Humaidi M, Chua C, Yeo G, Yap HM, Oh JQ, Manogaran V, Hapuarachchi HC, Maiwald M, Tee NWS, Barkham T, Koh TH, Gutiérrez RA, Schlundt J, Ng LC (2019) Salmonella in retail food and wild birds in Singapore—prevalence, antimicrobial resistance, and sequence types. Int J Environ Res Public Health 16:4235. https://doi.org/10.3390/ijerph16214235
doi: 10.3390/ijerph16214235
pubmed: 31683716
pmcid: 6862270
Ford L, Ellison Z, Schwensohn C, Griffin I, Birhane MG, Cote A, Fortenberry GZ, Tecle S, Higa J, Spencer S, Patton B, Patel J, Dow J, Maroufi A, Robbins A, Donovan D, Fitzgerald C, Burrell S, Tolar B, Folster JP, Cooley LA, Francois Watkins LK (2023) Strain of multidrug-resistant Salmonella Newport remains linked to travel to Mexico and U.S. beef products - United States, 2021-2022. MMWR Morb Mortal Wkly Rep 72:1225–1229. https://doi.org/10.15585/mmwr.mm7245a3
doi: 10.15585/mmwr.mm7245a3
pubmed: 37943708
pmcid: 10651324
Ricke SC, Kim SA, Shi Z (2018) Molecular-based identification and detection of Salmonella in food production systems: current perspectives | Journal of Applied Microbiology | Oxford Academic. J Appl Microbiol 125:313–327. https://doi.org/10.1111/jam.13888
doi: 10.1111/jam.13888
pubmed: 29675864
Blanco G, Tuesta JA (2018) Culture- and molecular-based detection of swine-adapted Salmonella shed by avian scavengers. Sci Total Environ 634:1513–1518. https://doi.org/10.1016/j.scitotenv.2018.04.089
doi: 10.1016/j.scitotenv.2018.04.089
pubmed: 29710649
Mainar-Jaime RC, Andrés S, Vico JP, San Román B, Garrido V, Grilló MJ (2020) Sensitivity of the ISO 6579:2002/Amd 1:2007 standard method for detection of Salmonella spp. on mesenteric lymph nodes from slaughter pigs. J Clin Microbiol 51:89–94. https://doi.org/10.1128/JCM.02099-12
doi: 10.1128/JCM.02099-12
Zhou Y-Y, Kang X-L, Meng C, Xiong D, Xu Y, Geng S-Z, Pan Z-M, Jiao X-A (2020) Multiple PCR assay based on the cigR gene for detection of Salmonella spp. and Salmonella pullorum/gallinarum identification. Poult Sci 99:5991–5998. https://doi.org/10.1016/j.psj.2020.07.026
doi: 10.1016/j.psj.2020.07.026
pubmed: 33142517
pmcid: 7647733
Rohde A, Hammerl JA, Boone I, Jansen W, Fohler S, Klein G, Dieckmann R, Al Dahouk S (2017) Overview of validated alternative methods for the detection of foodborne bacterial pathogens. Trends Food Sci Technol 62:113–118. https://doi.org/10.1016/j.tifs.2017.02.006
doi: 10.1016/j.tifs.2017.02.006
Gu K, Song Z, Zhou C, Ma P, Li C, Lu Q, Liao Z, Huang Z, Tang Y, Li H, Zhao Y, Yan W, Lei C, Wang H (2022) Development of nanobody-horseradish peroxidase-based sandwich ELISA to detect Salmonella enteritidis in milk and in vivo colonization in chicken. J Nanobiotechnology 20:167. https://doi.org/10.1186/s12951-022-01376-y
doi: 10.1186/s12951-022-01376-y
pubmed: 35361208
pmcid: 8973953
Zhang C, Liu Z, Bai M, Wang Y, Liao X, Zhang Y, Wang P, Wei J, Zhang H, Wang J, Wang H, Wang Y (2022) An ultrasensitive sandwich chemiluminescent enzyme immunoassay based on phage-mediated double-nanobody for detection of Salmonella typhimurium in food. Sens Actuators B Chem 352:131058. https://doi.org/10.1016/j.snb.2021.131058
doi: 10.1016/j.snb.2021.131058
Chen H, Qiu H, Zhong H, Cheng F, Wu Z, Shi T (2023) Non-typhoidal Salmonella infections among children in Fuzhou, Fujian, China: a 10-year retrospective review from 2012 to 2021. Infect Drug Resist 16:2737–2749. https://doi.org/10.2147/IDR.S408152
doi: 10.2147/IDR.S408152
pubmed: 37180635
pmcid: 10171219
Gong J, Kelly P, Wang C (2017) Prevalence and antimicrobial resistance of Salmonella enterica serovar Indiana in China (1984–2016). Zoonoses Public Health 64:239–251. https://doi.org/10.1111/zph.12328
doi: 10.1111/zph.12328
pubmed: 28009105
Zhang X, Lowe SB, Gooding JJ (2014) Brief review of monitoring methods for loop-mediated isothermal amplification (LAMP). Biosens Bioelectron 61:491–499. https://doi.org/10.1016/j.bios.2014.05.039
doi: 10.1016/j.bios.2014.05.039
pubmed: 24949822
Chen G-C, Liu C-H, Wu W-C (2021) Electrochemical immunosensor for serum parathyroid hormone using voltammetric techniques and a portable simulator. Anal Chim Acta 1143:84–92. https://doi.org/10.1016/j.aca.2020.11.045
doi: 10.1016/j.aca.2020.11.045
pubmed: 33384133
Ye F, Qian J, Xia J, Li L, Wang S, Zeng Z, Mao J, Ahamad M, Xiao Z, Zhang Q (2024) Efficient photoelectrocatalytic degradation of pollutants over hydrophobic carbon felt loaded with Fe-doped porous carbon nitride via direct activation of molecular oxygen. Environ Res 249:118497. https://doi.org/10.1016/j.envres.2024.118497
doi: 10.1016/j.envres.2024.118497
pubmed: 38365054
Yan J, Cheng Q, Liu H, Wang L, Yu K (2023) Sensitive and rapid detection of influenza A virus for disease surveillance using dual-probe electrochemical biosensor. Bioelectrochemistry Amst Neth 153:108497. https://doi.org/10.1016/j.bioelechem.2023.108497
doi: 10.1016/j.bioelechem.2023.108497
Hashem A, Hossain MAM, Marlinda AR, Mamun MA, Sagadevan S, Shahnavaz Z, Simarani K, Johan MR (2022) Nucleic acid-based electrochemical biosensors for rapid clinical diagnosis: advances, challenges, and opportunities. Crit Rev Clin Lab Sci 59:156–177. https://doi.org/10.1080/10408363.2021.1997898
doi: 10.1080/10408363.2021.1997898
pubmed: 34851806
Bolourinezhad M, Rezayi M, Meshkat Z, Soleimanpour S, Mojarrad M, Zibadi F, Aghaee-Bakhtiari SH, Taghdisi SM (2023) Design of a rapid electrochemical biosensor based on MXene/Pt/C nanocomposite and DNA/RNA hybridization for the detection of COVID-19. Talanta 265:124804. https://doi.org/10.1016/j.talanta.2023.124804
doi: 10.1016/j.talanta.2023.124804
pubmed: 37329753
pmcid: 10259158
Wu C, Chen Z, Li C, Hao Y, Tang Y, Yuan Y, Chai L, Fan T, Yu J, Ma X, Al-Hartomy OA, Wageh S, Al-Sehemi AG, Luo Z, He Y, Li J, Xie Z, Zhang H (2022) CRISPR-Cas12a-empowered electrochemical biosensor for rapid and ultrasensitive detection of SARS-CoV-2 delta variant. Nano-Micro Lett 14:159. https://doi.org/10.1007/s40820-022-00888-4
doi: 10.1007/s40820-022-00888-4
Li Q, Li Y, Gao Q, Jiang C, Tian Q, Ma C, Shi C (2022) Real-time monitoring of isothermal nucleic acid amplification on a smartphone by using a portable electrochemical device for home-testing of SARS-CoV-2. Anal Chim Acta 1229:340343. https://doi.org/10.1016/j.aca.2022.340343
doi: 10.1016/j.aca.2022.340343
pubmed: 36156220
pmcid: 9449873
Guan Z, Sun Y, Ma C-B, Lee JJ, Zhang S, Zhang X, Guo Z, Du Y (2023) Dual targets-induced specific hemin/G-quadruplex assemblies for label-free electrochemical detection capable of distinguishing Salmonella and its common serotype in food samples. Biosens Bioelectron 236:115438. https://doi.org/10.1016/j.bios.2023.115438
doi: 10.1016/j.bios.2023.115438
pubmed: 37263053
Li Z, Ding X, Yin K, Xu Z, Cooper K, Liu C (2021) Electric field-enhanced electrochemical CRISPR biosensor for DNA detection. Biosens Bioelectron 192:113498. https://doi.org/10.1016/j.bios.2021.113498
doi: 10.1016/j.bios.2021.113498
pubmed: 34280652
pmcid: 8453050
Foguel MV, Zamora V, Ojeda J, Reed M, Bennett A, Calvo-Marzal P, Gerasimova YV, Kolpashchikov D, Chumbimuni-Torres KY (2024) DNA nanotechnology for nucleic acid analysis: sensing of nucleic acids with DNA junction-probes. Analyst 149:968–974. https://doi.org/10.1039/D3AN01707A
doi: 10.1039/D3AN01707A
pubmed: 38197474
Pothipor C, Aroonyadet N, Bamrungsap S, Jakmunee J, Ounnunkad K (2021) A highly sensitive electrochemical microRNA-21 biosensor based on intercalating methylene blue signal amplification and a highly dispersed gold nanoparticles/graphene/polypyrrole composite. Analyst 146:2679–2688. https://doi.org/10.1039/D1AN00116G
doi: 10.1039/D1AN00116G
pubmed: 33687386
Kim HE, Schuck A, Lee SH, Lee Y, Kang M, Kim Y-S (2021) Sensitive electrochemical biosensor combined with isothermal amplification for point-of-care COVID-19 tests. Biosens Bioelectron 182:113168. https://doi.org/10.1016/j.bios.2021.113168
doi: 10.1016/j.bios.2021.113168
pubmed: 33780853
pmcid: 7970423
Yuan R, Wei J, Geng R, Li B, Xiong W, Fang X, Wang K (2023) Sensitive detection of African swine fever virus p54 based on in-situ amplification of disposable electrochemical sensor chip. Sens Actuators B Chem 380:133363. https://doi.org/10.1016/j.snb.2023.133363
doi: 10.1016/j.snb.2023.133363
Zheng S, Yang Q, Yang H, Zhang Y, Guo W, Zhang W (2023) An ultrasensitive and specific ratiometric electrochemical biosensor based on SRCA-CRISPR/Cas12a system for detection of Salmonella in food. Food Control 146:109528. https://doi.org/10.1016/j.foodcont.2022.109528
doi: 10.1016/j.foodcont.2022.109528
Zhu D, Yan Y, Lei P, Shen B, Cheng W, Ju H, Ding S (2014) A novel electrochemical sensing strategy for rapid and ultrasensitive detection of Salmonella by rolling circle amplification and DNA–AuNPs probe. Anal Chim Acta 846:44–50. https://doi.org/10.1016/j.aca.2014.07.024
doi: 10.1016/j.aca.2014.07.024
pubmed: 25220140
Wang C, Xu Z, Hou X, Wang M, Zhou C, Liang J, Wei P (2022) Rapid, sensitive, specific, and visual detection of Salmonella in retail meat with loop-mediated isothermal amplification, targeting the invA gene. J Food Prot 85:6–12. https://doi.org/10.4315/JFP-21-186
doi: 10.4315/JFP-21-186
pubmed: 34436593
Shatleh-Rantisi D, Tamimi A, Ashhab Y (2020) Improving sensitivity of single tube nested PCR to detect fastidious microorganisms. Heliyon 6:e03246. https://doi.org/10.1016/j.heliyon.2020.e03246
doi: 10.1016/j.heliyon.2020.e03246
pubmed: 32042975
pmcid: 7002839
Valdés-Aguayo JJ, Garza-Veloz I, Vargas-Rodríguez JR, Martinez-Vazquez MC, Avila-Carrasco L, Bernal-Silva S, González-Fuentes C, Comas-García A, Alvarado-Hernández DE, Centeno-Ramirez ASH, Rodriguez-Sánchez IP, Delgado-Enciso I, Martinez-Fierro ML (2021) Peripheral blood mitochondrial DNA levels were modulated by SARS-CoV-2 infection severity and its lessening was associated with mortality among hospitalized patients with COVID-19. Front Cell Infect Microbiol 11. https://doi.org/10.3389/fcimb.2021.754708
Argoubi W, Saadaoui M, Aoun SB, Raouafi N (2015) Optimized design of a nanostructured SPCE-based multipurpose biosensing platform formed by ferrocene-tethered electrochemically-deposited cauliflower-shaped gold nanoparticles. Beilstein J Nanotechnol 6:1840–1852. https://doi.org/10.3762/bjnano.6.187
doi: 10.3762/bjnano.6.187
pubmed: 26425435
pmcid: 4578399
Yu Z, Luan Y, Li H, Wang W, Wang X, Zhang Q (2019) A disposable electrochemical aptasensor using single-stranded DNA–methylene blue complex as signal-amplification platform for sensitive sensing of bisphenol A. Sens Actuators B Chem 284:73–80. https://doi.org/10.1016/j.snb.2018.12.126
doi: 10.1016/j.snb.2018.12.126
Luo Z, Xu Y, Huang Z, Chen J, Wang X, Li D, Li Y, Duan Y (2020) A rapid, adaptative DNA biosensor based on molecular beacon-concatenated dual signal amplification strategies for ultrasensitive detection of p53 gene and cancer cells. Talanta 210:120638. https://doi.org/10.1016/j.talanta.2019.120638
doi: 10.1016/j.talanta.2019.120638
pubmed: 31987215
Li J, Zhao F, Zhao J, Zeng B (2005) Adsorptive and stripping behavior of methylene blue at gold electrodes in the presence of cationic gemini surfactants. Electrochim Acta 51:297–303. https://doi.org/10.1016/j.electacta.2005.04.024
doi: 10.1016/j.electacta.2005.04.024
Chen Y, Shi Y, Chen Y, Yang Z, Wu H, Zhou Z, Li J, Ping J, He L, Shen H, Chen Z, Wu J, Yu Y, Zhang Y, Chen H (2020) Contamination-free visual detection of SARS-CoV-2 with CRISPR/Cas12a: a promising method in the point-of-care detection. Biosens Bioelectron 169:112642. https://doi.org/10.1016/j.bios.2020.112642
doi: 10.1016/j.bios.2020.112642
pubmed: 32979593
pmcid: 7502227
Wang N, Zhang J, Xiao B, Li H, Chen J, Sun X, Huang F, Li H, Chen A (2023) Integration of in-cassette lysis, purification, and lateral flow strips-based sensor for rapid and on-site detection of yak milk adulteration. Sens Actuators B Chem 394:134309. https://doi.org/10.1016/j.snb.2023.134309
doi: 10.1016/j.snb.2023.134309
Fortunati S, Vasini I, Giannetto M, Mattarozzi M, Porchetta A, Bertucci A, Careri M (2022) Controlling dynamic DNA reactions at the surface of single-walled carbon nanotube electrodes to design hybridization platforms with a specific amperometric readout. Anal Chem 94:5075–5083. https://doi.org/10.1021/acs.analchem.1c05294
doi: 10.1021/acs.analchem.1c05294
pubmed: 35303407
pmcid: 8968946
Hanpanich O, Lomae A, Maruyama A, Palaga T, Chailapakul O, Ngamrojanavanich N (2023) Label-free detection of HPV mRNA with an artificial chaperone-enhanced MNAzyme (ACEzyme)-based electrochemical sensor. Biosens Bioelectron 221:114352. https://doi.org/10.1016/j.bios.2022.114352
doi: 10.1016/j.bios.2022.114352
pubmed: 35690559
Huang X, Niu W, Wu J, Wang Y, Li C, Qiu J, Xue J (2019) A triple-amplification differential pulse voltammetry for sensitive detection of DNA based on exonuclease III, strand displacement reaction and terminal deoxynucleotidyl transferase. Biosens Bioelectron 143:111609. https://doi.org/10.1016/j.bios.2019.111609
doi: 10.1016/j.bios.2019.111609
pubmed: 31476597
Tang J, Zou G, Chen C, Ren J, Wang F, Chen Z (2021) Highly selective electrochemical detection of 5-formyluracil relying on (2-benzimidazolyl) acetonitrile labeling. Anal Chem 93:16439–16446. https://doi.org/10.1021/acs.analchem.1c03389
doi: 10.1021/acs.analchem.1c03389
pubmed: 34813282
Fortunati S, Rozzi A, Curti F, Giannetto M, Corradini R, Careri M (2019) Novel amperometric genosensor based on peptide nucleic acid (PNA) probes immobilized on carbon nanotubes-screen printed electrodes for the determination of trace levels of non-amplified DNA in genetically modified (GM) soy. Biosens Bioelectron 129:7–14. https://doi.org/10.1016/j.bios.2019.01.020
doi: 10.1016/j.bios.2019.01.020
pubmed: 30682690