A rho-type GTPase activating protein affects the growth and development of Cordyceps cicadae.
Cordyceps cicadae
Active components
Growth and development
Rho-type GTPase activating protein
Journal
Archives of microbiology
ISSN: 1432-072X
Titre abrégé: Arch Microbiol
Pays: Germany
ID NLM: 0410427
Informations de publication
Date de publication:
03 Jul 2024
03 Jul 2024
Historique:
received:
29
04
2024
accepted:
23
06
2024
revised:
13
06
2024
medline:
3
7
2024
pubmed:
3
7
2024
entrez:
3
7
2024
Statut:
epublish
Résumé
Cordyceps cicadae is recognized for its medicinal properties, attributed to bioactive constituents like polysaccharides and adenosine, which have been shown to improve kidney and liver functions and possess anti-tumor properties. Rho GTPase activating proteins (Rho GAPs) serve as inhibitory regulators of Rho GTPases in eukaryotic cells by accelerating the GTP hydrolysis of Rho GTPases, leading to their inactivation. In this study, we explored the function of the CcRga8 gene in C. cicadae, which encodes a Rho-type GTPase activating protein. Our study found that the knockout of CcRga8 resulted in a decrease in polysaccharide levels and an increase in adenosine concentration. Furthermore, the mutants exhibited altered spore yield and morphology, fruiting body development, decreased infectivity, reduced resistance to hyperosmotic stress, oxidative conditions, and cell wall inhibitors. These findings suggest that CcRga8 plays a crucial role in the development, stress response, and bioactive compound production of C. cicadae.
Identifiants
pubmed: 38958759
doi: 10.1007/s00203-024-04072-7
pii: 10.1007/s00203-024-04072-7
doi:
Substances chimiques
GTPase-Activating Proteins
0
Fungal Proteins
0
rho GTPase-activating protein
0
Adenosine
K72T3FS567
Polysaccharides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
339Subventions
Organisme : State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products
ID : No. 2021DG700024-KF202315
Organisme : State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products
ID : No. 2021DG700024-KF202315
Organisme : State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products
ID : No. 2021DG700024-KF202315
Organisme : State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products
ID : No. 2021DG700024-KF202315
Organisme : State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products
ID : No. 2021DG700024-KF202315
Organisme : State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products
ID : No. 2021DG700024-KF202315
Organisme : Zhejiang Science and Technology Major Program on Agriculture New Variety Breeding
ID : No. 2021C02073-9
Organisme : Zhejiang Science and Technology Major Program on Agriculture New Variety Breeding
ID : No. 2021C02073-9
Organisme : Zhejiang Science and Technology Major Program on Agriculture New Variety Breeding
ID : No. 2021C02073-9
Organisme : Zhejiang Science and Technology Major Program on Agriculture New Variety Breeding
ID : No. 2021C02073-9
Organisme : Zhejiang Science and Technology Major Program on Agriculture New Variety Breeding
ID : No. 2021C02073-9
Organisme : Zhejiang Science and Technology Major Program on Agriculture New Variety Breeding
ID : No. 2021C02073-9
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Références
Adams AE, Johnson DI, Longnecker RM, Sloat BF, Pringle JR (1990) CDC42 and CDC43, two additional genes involved in budding and the establishment of cell polarity in the yeast Saccharomyces cerevisiae. J Cell Biol 111:131–142. https://doi.org/10.1083/jcb.111.1.131
doi: 10.1083/jcb.111.1.131
pubmed: 2195038
Ailin H (2021) Analysis and diversity of microorganism community of wild Cicada Flowers and their habitats in Anji, Zhejiang. Dissertation, Tianjin University of Commerce
Altschul SF et al (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402. https://doi.org/10.1093/nar/25.17.3389
doi: 10.1093/nar/25.17.3389
pubmed: 9254694
pmcid: 146917
Andrianopoulos A et al (2008) Rac1 is required for pathogenicity and Chm1-dependent conidiogenesis in rice fungal pathogen Magnaporthe Grisea. PLOS Pathog 4:e1000202. https://doi.org/10.1371/journal.ppat.1000202
doi: 10.1371/journal.ppat.1000202
Bement WM, Goryachev AB, Miller AL, von Dassow G (2024) Patterning of the cell cortex by rho GTPases. Nat Rev Mol Cell Biol 25:290–308. https://doi.org/10.1038/s41580-023-00682-z
doi: 10.1038/s41580-023-00682-z
pubmed: 38172611
Dautt-Castro M, Rosendo-Vargas M, Casas-Flores S (2021) The small GTPases in fungal signaling conservation and function. Cells 10:1039. https://doi.org/10.3390/cells10051039
doi: 10.3390/cells10051039
pubmed: 33924947
pmcid: 8146680
Dong C, Guo S, Wang W, Liu X (2015) Cordyceps industry in China. Mycology 6:121–129. https://doi.org/10.1080/21501203.2015.1043967
doi: 10.1080/21501203.2015.1043967
pubmed: 30151320
pmcid: 6106062
Garrett MD, Major GN, Totty N, Hall A (1991) Purification and N-terminal sequence of the p21rho GTPase-activating protein, Rho GAP. Biochem J 276:833–836. https://doi.org/10.1042/bj2760833
doi: 10.1042/bj2760833
pubmed: 1905930
pmcid: 1151079
Ho Y, Ren X, He L, Cheng J, Chang J (2014) Optimization of the solid-state fermentation and properties of a polysaccharide from Paecilomyces cicadae (Miquel) Samson and its antioxidant activities in vitro. PLoS ONE 9:e87578. https://doi.org/10.1371/journal.pone.0087578
doi: 10.1371/journal.pone.0087578
Huang X (2020) Liquid fermentation and active ingredient determination of lsaria cicadae. Jiangsu Agricultural Sci 48:197–201. https://doi.org/10.15889/j.issn.1002-1302.2020.02.036
doi: 10.15889/j.issn.1002-1302.2020.02.036
Hwang J, Vernoud V, Szumlanski A, Nielsen E, Yang Z (2008) A tip-localized RhoGAP controls cell polarity by globally inhibiting rho GTPase at the cell apex. Curr Biol 18:1907–1916. https://doi.org/10.1016/j.cub.2008.11.057
doi: 10.1016/j.cub.2008.11.057
pubmed: 19108776
pmcid: 2615002
Layland J, Carrick D, Lee M, Oldroyd K, Berry C (2014) Adenosine: physiology, pharmacology, and clinical applications. Jacc-Cardiovasc Inte 7:581–591. https://doi.org/10.1016/j.jcin.2014.02.009
doi: 10.1016/j.jcin.2014.02.009
Li HJ, Lu JP, Liu XH, Zhang LL, Lin FC (2012) Vectors building and usage for gene knockout, protein expression and fluorescent Fusion protein in the Rice Blast Fungus. 20:94–104. https://doi.org/10.3969/j.issn.1674-7968.2012.01.013
Mahlert M, Leveleki L, Hlubek A, Sandrock B, Bölker M (2005) Rac1 and Cdc42 regulate hyphal growth and cytokinesis in the dimorphic fungus Ustilago maydis. Mol Microbiol 59:567–578. https://doi.org/10.1111/j.1365-2958.2005.04952.x
doi: 10.1111/j.1365-2958.2005.04952.x
Miller PJ, Johnson DI (1994) Cdc42p GTPase is involved in controlling polarized cell growth in Schizosaccharomyces Pombe. Mol Cell Biol 14:1075–1083. https://doi.org/10.1128/mcb.14.2.1075
doi: 10.1128/mcb.14.2.1075
pubmed: 8289788
pmcid: 358463
Nahm M, Lee S (2011) Characterization of the rho GTPase-Activating protein RhoGAP68F. Exp. Neurobiol 20:29–34. https://doi.org/10.5607/en.2011.20.1.29
doi: 10.5607/en.2011.20.1.29
Nobes CD, Hall A (1995) Rho, Rac, and Cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell 81:53–62. https://doi.org/10.1016/0092-8674(95)90370-4
doi: 10.1016/0092-8674(95)90370-4
pubmed: 7536630
Nxumalo W, Elateeq AA, Sun Y (2020) Can cordyceps cicadae be used as an alternative to Cordyceps militaris and cordyceps sinensis? - a review. J Ethnopharmacol 257:112879. https://doi.org/10.1016/j.jep.2020.112879
doi: 10.1016/j.jep.2020.112879
pubmed: 32305637
Qi Z, Du Y, Liu Y (2015) Role of rho GTPase-activated protein MoBem2 in conidium morphogenesis of Magnaporthe oryzae. Jiangsu J Agricultural Sci 31:1001–1005. https://doi.org/10.3969/j.issn.1000-4440.2015.05.009
doi: 10.3969/j.issn.1000-4440.2015.05.009
Qin W, Mu C, Yin S (2019a) Research progress of chemical components and pharmacological effects of lsaria cicadae Miquel. Chin J Libr Inform Sci Traditional Chin Med 43:73–76. https://doi.org/10.3969/j.issn.2095-5707.2019.04.019
Qin L, Yin B, Ren P, Wang J, Wang Y, Wang C (2019b) Effects of lsaria cicadae fruiting bodies on renal tubular epithelial cells in rats. Mycosystema 38:1501–1509. https://doi.org/10.13346/j.mycosystema.190142
doi: 10.13346/j.mycosystema.190142
Qu Y et al (2022) A kelch domain cell end protein, PoTea1, mediates cell polarization during appressorium morphogenesis in Pyricularia oryzae. Microbiol Res 259:126999. https://doi.org/10.1016/j.micres.2022.126999
doi: 10.1016/j.micres.2022.126999
pubmed: 35305442
Schäffer AA et al (2001) Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements. Nucleic Acids Res 29:2994–3005. https://doi.org/10.1093/nar/29.14.2994
doi: 10.1093/nar/29.14.2994
pubmed: 11452024
pmcid: 55814
Schultz J, Milpetz F, Bork P, Ponting CP (1998) SMART, a simple modular architecture research tool: identification of signaling domains. PNAS 95:5857–5864. https://doi.org/10.1073/pnas.95.11.5857
doi: 10.1073/pnas.95.11.5857
pubmed: 9600884
pmcid: 34487
Soto T et al (2010) Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein. J Biol Chem 285:11516–11525. https://doi.org/10.1074/jbc.M109.071027
doi: 10.1074/jbc.M109.071027
pubmed: 20164182
pmcid: 2857030
Sun Y et al (2017) Isaria Cicadae conidia possess antiproliferative and inducing apoptosis properties in gynaecological carcinoma cells. Mycology 8:327–334. https://doi.org/10.1080/21501203.2017.1386243
doi: 10.1080/21501203.2017.1386243
pubmed: 30123653
pmcid: 6059127
Vijayan D, Young A, Teng MWL, Smyth MJ (2017) Targeting immunosuppressive adenosine in cancer. Nat Rev Cancer 17:709–724. https://doi.org/10.1038/nrc.2017.86
doi: 10.1038/nrc.2017.86
pubmed: 29059149
Virag A, Lee MP, Si H, Harris SD (2007) Regulation of hyphal morphogenesis by cdc42 and rac1 homologues in aspergillus nidulans. Mol Microbiol 66:1579–1596. https://doi.org/10.1111/j.1365-2958.2007.06021.x
doi: 10.1111/j.1365-2958.2007.06021.x
pubmed: 18005099
Visagie CM et al (2014) Identification and nomenclature of the genus Penicillium. Stud Mycol 78:343–371. https://doi.org/10.1016/j.simyco.2014.09.001
doi: 10.1016/j.simyco.2014.09.001
pubmed: 25505353
pmcid: 4261876
Vogt N, Seiler S, Chang F (2008) The RHO1-specific GTPase-activating protein LRG1 regulates polar tip growth in parallel to ndr kinase signaling in Neurospora. Mol Biol Cell 19:4554–4569. https://doi.org/10.1091/mbc.e07-12-1266
doi: 10.1091/mbc.e07-12-1266
pubmed: 18716060
pmcid: 2575149
Wang H, Zhang J, Sit W, Lee C, Wan J (2014) Cordyceps Cicadae induces G2/M cell cycle arrest in MHCC97H human hepatocellular carcinoma cells: a proteomic study. Chin Med 9:15. https://doi.org/10.1186/1749-8546-9-15
doi: 10.1186/1749-8546-9-15
pubmed: 24872842
pmcid: 4036300
Wang D, Zhu X, Gao L, Chen D (2019) Comparative analyses of different solvent extracts of lsaria cicadae on obesity control using a mouse model. Mycosystema 38:1689–1701. https://doi.org/10.13346/j.mycosystema.190197
doi: 10.13346/j.mycosystema.190197
Wei Y, Yang M, Zou X, Liu A (2014) Review on bioactive components from lsaria cicadae. Guizhou Agricultural Sci 42:142–148. https://doi.org/10.3969/j.issn.1001-3601.2014.12.034
doi: 10.3969/j.issn.1001-3601.2014.12.034
Wei C et al (2016) Structure and chain conformation of a neutral intracellular heteropolysaccharide from mycelium of Paecilomyces cicadae. Carbohydr Polym 136:728–737. https://doi.org/10.1016/j.carbpol.2015.09.088
doi: 10.1016/j.carbpol.2015.09.088
pubmed: 26572406
Wu T et al (2019) A single transcription factor (PDD1) determines development and yield of winter mushroom (Flammulina Velutipes). Appl Environ Microbiol 85:e01735–e01719. https://doi.org/10.1128/aem.01735-19
doi: 10.1128/aem.01735-19
pubmed: 31604770
pmcid: 6881794
Wu X et al (2021) The expression pattern of CWl and HOG signal pathway genes during the growth and development of Hypsizygus marmoreus. Mycosystema 40:1388–1399. https://doi.org/10.13346/j.mycosystema.200393
doi: 10.13346/j.mycosystema.200393
Xie S et al (2020) Research progress of cicada flower and its components in enhancing immunity and antitumor pharmacology. Drug Eval Res 43:624–629. https://doi.org/10.7501/j.issn.1674-6376.2020.04.007
Yang Q, Xiao L, Huang X, Zhang G, Yu N, Tan J (2016) Polysaccharide content and composition analysis of Golden Cicada flowers and effect on NF-κB activity of LPS inducted THP-1 cells. Mod Chin Med 18:1129–1134. https://doi.org/10.13313/j.issn.1673-4890.2016.9.010
doi: 10.13313/j.issn.1673-4890.2016.9.010
Ye W et al (2014) Putative RhoGAP proteins orchestrate vegetative growth, conidiogenesis and pathogenicity of the rice blast fungus Magnaporthe oryzae. Fungal Genet Biol 67:37–50. https://doi.org/10.1016/j.fgb.2014.03.008
doi: 10.1016/j.fgb.2014.03.008
pubmed: 24731806
Ye W, Yu W, Lu G (2017) Progress of researches on RhoGAP domain protein family. Genomics Appl Biology 36:2573–2580. https://doi.org/10.13417/j.gab.036.002573
doi: 10.13417/j.gab.036.002573
Yue F, Zhang J, Xu J, Niu T, Lü X, Liu M (2022) Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method. Front Nutr 9:963318. https://doi.org/10.3389/fnut.2022.963318
doi: 10.3389/fnut.2022.963318
pubmed: 35983486
pmcid: 9378961
Zhang X (2019) Role of small GTPase Rho1 in cell wall integrity, stress response and pathogenesis of Aspergillus fumigatus. Dissertation, Academy of Military Sciences 6.Statements & Declarations
Zheng W et al (2007) A Rho3 homolog is essential for appressorium development and pathogenicity of magnaporthe grisea. Eukaryot Cell 6:2240–2250. https://doi.org/10.1128/ec.00104-07
doi: 10.1128/ec.00104-07
pubmed: 17933908
pmcid: 2168240