Syringin from Tinospora crispa downregulates pro-inflammatory mediator production through MyD88-dependent pathways in lipopolysaccharide (LPS)-induced U937 macrophages.
Humans
Myeloid Differentiation Factor 88
/ metabolism
Macrophages
/ drug effects
Lipopolysaccharides
/ pharmacology
Signal Transduction
/ drug effects
Tinospora
/ chemistry
Glucosides
/ pharmacology
Phenylpropionates
/ pharmacology
NF-kappa B
/ metabolism
U937 Cells
Dinoprostone
/ metabolism
Interleukin-1beta
/ metabolism
Down-Regulation
/ drug effects
Cyclooxygenase 2
/ metabolism
Inflammation Mediators
/ metabolism
Tumor Necrosis Factor-alpha
/ metabolism
Proto-Oncogene Proteins c-akt
/ metabolism
Phosphatidylinositol 3-Kinases
/ metabolism
Toll-Like Receptor 4
/ metabolism
Anti-inflammatory
MAPKs
Macrophages
NF‐кB
PI3K-Akt
Syringin
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
11 Jul 2024
11 Jul 2024
Historique:
received:
13
03
2024
accepted:
12
06
2024
medline:
11
7
2024
pubmed:
11
7
2024
entrez:
11
7
2024
Statut:
epublish
Résumé
Syringin, a phenylpropanoid glycoside, has exhibited numerous biological properties including inhibitory activities against various immune and inflammatory disorders. In this study, syringin isolated from Tinospora crispa was evaluated for its ability to down-regulate activated nuclear factor-kappa B (NF-κB), phosphoinositide-3-kinase-Akt (PI3K-Akt) and mitogen-activated protein kinases (MAPKs) signal transducing networks in U937 macrophages activated by lipopolysaccharide. The attenuating effects of syringin on the productions of prostaglandin E2 (PGE2), cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α), and the expressions of signaling molecules of the signaling pathways were investigated by using ELISA, Western blot, and qRT-PCR. Syringin downregulated the NF-κB, MAPKs, and PI3K-Akt signal networks by significantly reducing PGE The suppressive effect of syringin on the inflammatory signaling molecules in MyD88-dependent pathways suggested it's potential as a drug candidate for development into an agent for treatment of various immune-mediated inflammatory disorders.
Sections du résumé
BACKGROUND
BACKGROUND
Syringin, a phenylpropanoid glycoside, has exhibited numerous biological properties including inhibitory activities against various immune and inflammatory disorders. In this study, syringin isolated from Tinospora crispa was evaluated for its ability to down-regulate activated nuclear factor-kappa B (NF-κB), phosphoinositide-3-kinase-Akt (PI3K-Akt) and mitogen-activated protein kinases (MAPKs) signal transducing networks in U937 macrophages activated by lipopolysaccharide.
METHODS
METHODS
The attenuating effects of syringin on the productions of prostaglandin E2 (PGE2), cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α), and the expressions of signaling molecules of the signaling pathways were investigated by using ELISA, Western blot, and qRT-PCR.
RESULTS
RESULTS
Syringin downregulated the NF-κB, MAPKs, and PI3K-Akt signal networks by significantly reducing PGE
CONCLUSION
CONCLUSIONS
The suppressive effect of syringin on the inflammatory signaling molecules in MyD88-dependent pathways suggested it's potential as a drug candidate for development into an agent for treatment of various immune-mediated inflammatory disorders.
Identifiants
pubmed: 38990383
doi: 10.1007/s11033-024-09722-z
pii: 10.1007/s11033-024-09722-z
doi:
Substances chimiques
syringin
I6F5B11C96
Myeloid Differentiation Factor 88
0
Lipopolysaccharides
0
Glucosides
0
Phenylpropionates
0
NF-kappa B
0
Dinoprostone
K7Q1JQR04M
MYD88 protein, human
0
Interleukin-1beta
0
Cyclooxygenase 2
EC 1.14.99.1
Inflammation Mediators
0
Tumor Necrosis Factor-alpha
0
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Phosphatidylinositol 3-Kinases
EC 2.7.1.-
Toll-Like Receptor 4
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
789Subventions
Organisme : Kementerian Pertanian dan Industri Asas Tani Malaysia
ID : NH1015D075
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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