Metagenomic Analysis of Rhizospheric Bacterial Community of Citrus Trees Expressing Phloem-Directed Antimicrobials.
Rhizosphere
Phloem
/ microbiology
Soil Microbiology
Bacteria
/ genetics
Microbiota
Plant Diseases
/ microbiology
Citrus
/ microbiology
Plants, Genetically Modified
/ microbiology
Phylogeny
Metagenomics
Muramidase
/ metabolism
Plant Proteins
/ genetics
beta-Defensins
/ genetics
RNA, Ribosomal, 16S
/ genetics
Anti-Infective Agents
/ pharmacology
Citrus sinensis
/ microbiology
Plant Roots
/ microbiology
HLB mitigation
Microbial diversity
Phloem-directed antimicrobials
Transgenic citrus
Journal
Microbial ecology
ISSN: 1432-184X
Titre abrégé: Microb Ecol
Pays: United States
ID NLM: 7500663
Informations de publication
Date de publication:
15 Jul 2024
15 Jul 2024
Historique:
received:
27
04
2024
accepted:
05
07
2024
medline:
15
7
2024
pubmed:
15
7
2024
entrez:
15
7
2024
Statut:
epublish
Résumé
Huanglongbing, also known as citrus greening, is currently the most devastating citrus disease with limited success in prevention and mitigation. A promising strategy for Huanglongbing control is the use of antimicrobials fused to a carrier protein (phloem protein of 16 kDa or PP16) that targets vascular tissues. This study investigated the effects of genetically modified citrus trees expressing Citrus sinensis PP16 (CsPP16) fused to human lysozyme and β-defensin-2 on the soil microbiome diversity using 16S amplicon analysis. The results indicated that there were no significant alterations in alpha diversity, beta diversity, phylogenetic diversity, differential abundance, or functional prediction between the antimicrobial phloem-overexpressing plants and the control group, suggesting minimal impact on microbial community structure. However, microbiota diversity analysis revealed distinct bacterial assemblages between the rhizosphere soil and root environments. This study helps to understand the ecological implications of crops expressing phloem-targeted antimicrobials for vascular disease management, with minimal impact on soil microbiota.
Identifiants
pubmed: 39008123
doi: 10.1007/s00248-024-02408-w
pii: 10.1007/s00248-024-02408-w
doi:
Substances chimiques
Muramidase
EC 3.2.1.17
Plant Proteins
0
beta-Defensins
0
RNA, Ribosomal, 16S
0
Anti-Infective Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
93Subventions
Organisme : Servicio Nacional de Sanidad e Inocuidad Agroalimentaria (SENASICA)
ID : SENASICA-CINVESTAV 2017
Organisme : Servicio Nacional de Sanidad e Inocuidad Agroalimentaria (SENASICA)
ID : SENASICA-CINVESTAV 2017
Organisme : Servicio Nacional de Sanidad e Inocuidad Agroalimentaria (SENASICA)
ID : SENASICA-CINVESTAV 2017
Organisme : Servicio Nacional de Sanidad e Inocuidad Agroalimentaria (SENASICA)
ID : SENASICA-CINVESTAV 2017
Organisme : Consejo Nacional de Humanidades, Ciencias y Tecnologías
ID : CF-2023-G-231
Informations de copyright
© 2024. The Author(s).
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