VlMYB4 and VlCDF3 co-targeted the VlLOG11 promoter to regulate fruit setting in grape (Vitis vinifera L).
Vitis
/ genetics
Gene Expression Regulation, Plant
Fruit
/ genetics
Plant Proteins
/ genetics
Promoter Regions, Genetic
/ genetics
Cytokinins
/ metabolism
Plants, Genetically Modified
Transcription Factors
/ genetics
Solanum lycopersicum
/ genetics
Phenylurea Compounds
/ pharmacology
Signal Transduction
/ genetics
Pyridines
Cytokinin
Functions
Grape
VlLOG11
VlMYB4/VlCDF3-VlLOG11
Journal
Plant cell reports
ISSN: 1432-203X
Titre abrégé: Plant Cell Rep
Pays: Germany
ID NLM: 9880970
Informations de publication
Date de publication:
15 Jul 2024
15 Jul 2024
Historique:
received:
26
03
2024
accepted:
09
07
2024
medline:
15
7
2024
pubmed:
15
7
2024
entrez:
15
7
2024
Statut:
epublish
Résumé
The VlLOG11 mediates the cytokinin signaling pathway to regulate grape fruit setting. Fruit set, as an accepted agronomic trait, is inextricably linked with fruit quality and yield. Previous studies have demonstrated that exogenous treatment with the synthetic cytokinin analog, forchlorfenuron (CPPU), significantly enhances fruit set. In this study, a significant reduction in endogenous cytokinins was found by measuring the content of cytokinins in young grape berries after CPPU treatment. LONELY GUYs (VlLOGs), a key cytokinin-activating enzyme working in the biosynthesis pathway of cytokinins, exhibited differential expression. Some differentially expressed VlLOGs genes were presented by RNA seq data and their functions and regulation patterns were further investigated. The results showed that VlLOG11 was differentially expressed in young grape berries after CPPU treatment. Overexpression of VlLOG11 in tomato increases the amount of fruit set, and upregulated the expression of genes associated with cytokinin signaling including SlHK4, SlHK5, SlHP3, SlHP4, SlPHP1, SlPHP2. VlMYB4 and VlCDF3 could regulate the expression of VlLOG11 by directly binding to its promoter in young grape berries during fruit set. These results strongly demonstrated that VlMYB4/VlCDF3-VlLOG11 regulatory module plays a key role in the process of fruit setting in grape. This provided a basis for the molecular mechanism of VlLOG11-mediated cytokinin biosynthesis in young grape fruit set.
Identifiants
pubmed: 39008131
doi: 10.1007/s00299-024-03279-8
pii: 10.1007/s00299-024-03279-8
doi:
Substances chimiques
Plant Proteins
0
Cytokinins
0
Transcription Factors
0
Phenylurea Compounds
0
N-(2-chloro-4-pyridyl)-N'-phenylurea
K62IP7463J
Pyridines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
194Subventions
Organisme : National Natural Science Foundation of China
ID : (Grant no. 32072517)
Organisme : Science and Technology Innovation Talents in Universities of Henan Province
ID : (21HASTIT035)
Organisme : Top Young Talents in Central Plains
ID : (No. Yuzutong (2021)44)
Organisme : Luoyang City Science and Technology Planning Project
ID : (2101102A)
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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