Precision control of ammonium release in Azotobacter vinelandii.
Journal
Microbial biotechnology
ISSN: 1751-7915
Titre abrégé: Microb Biotechnol
Pays: United States
ID NLM: 101316335
Informations de publication
Date de publication:
Jul 2024
Jul 2024
Historique:
received:
30
03
2024
accepted:
27
06
2024
medline:
18
7
2024
pubmed:
18
7
2024
entrez:
18
7
2024
Statut:
ppublish
Résumé
The capture and reduction of atmospheric dinitrogen gas to ammonium can be accomplished through the enzyme nitrogenase in a process known as biological nitrogen fixation (BNF), by a class of microbes known as diazotrophs. The diazotroph Azotobacter vinelandii is a model organism for the study of aerobic nitrogen fixation, and in recent years has been promoted as a potential producer of biofertilizers. Prior reports have demonstrated the potential to partially deregulate BNF in A. vinelandii, resulting in accumulation and extracellular release of ammonium. In many cases, deregulation requires the introduction of transgenic genes or elements to yield the desired phenotype, and the long-term stability of these strains has been reported to be somewhat problematic. In this work, we constructed two strains of A. vinelandii where regulation can be precisely controlled without the addition of any foreign genes or genetic markers. Regulation is maintained through native promoters found in A. vinelandii that can be induced through the addition of extraneous galactose. These strains result in varied degrees of regulation of BNF, and as a result, the release of extracellular ammonium is controlled in a precise, and galactose concentration-dependent manner. In addition, these strains yield high biomass levels, similar to the wild-type A. vinelandii strain and are further able to produce high percentages of the bioplastic polyhydroxybutyrate.
Identifiants
pubmed: 39023513
doi: 10.1111/1751-7915.14523
doi:
Substances chimiques
Ammonium Compounds
0
poly-beta-hydroxybutyrate
26063-00-3
Hydroxybutyrates
0
Polyhydroxybutyrates
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14523Subventions
Organisme : U.S. Department of Agriculture
ID : 2020-67019-31148
Organisme : National Institute of Food and Agriculture
ID : MIN-12-070
Organisme : National Institute of Food and Agriculture
ID : MIN-12-081
Informations de copyright
© 2024 The Author(s). Microbial Biotechnology published by John Wiley & Sons Ltd.
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