Upstream CtrA-binding sites both induce and repress pilin gene expression in Caulobacter crescentus.
Caulobacter crescentus
pilA
CtrA
Transcriptional regulation
Journal
BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258
Informations de publication
Date de publication:
19 Jul 2024
19 Jul 2024
Historique:
received:
12
02
2024
accepted:
17
06
2024
medline:
20
7
2024
pubmed:
20
7
2024
entrez:
19
7
2024
Statut:
epublish
Résumé
Pili are bacterial surface structures important for surface adhesion. In the alphaproteobacterium Caulobacter crescentus, the global regulator CtrA activates transcription of roughly 100 genes, including pilA which codes for the pilin monomer that makes up the pilus filament. While most CtrA-activated promoters have a single CtrA-binding site at the - 35 position and are induced at the early to mid-predivisional cell stage, the pilA promoter has 3 additional upstream CtrA-binding sites and it is induced at the late predivisional cell stage. Reporter constructs where these additional sites were disrupted by deletion or mutation led to increased activity compared to the WT promoter. In synchronized cultures, these mutations caused pilA transcription to occur approximately 20 min earlier than WT. The results suggested that the site overlapping the - 35 position drives pilA gene expression while the other upstream CtrA-binding sites serve to reduce and delay expression. EMSA experiments showed that the - 35 Site has lower affinity for CtrA∼P compared to the other sites, suggesting binding site affinity may be involved in the delay mechanism. Mutating the upstream inhibitory CtrA-binding sites in the pilA promoter caused significantly higher numbers of pre-divisional cells to express pili, and phage survival assays showed this strain to be significantly more sensitive to pilitropic phage. These results suggest that pilA regulation evolved in C. crescentus to provide an ecological advantage within the context of phage infection.
Identifiants
pubmed: 39030481
doi: 10.1186/s12864-024-10533-6
pii: 10.1186/s12864-024-10533-6
doi:
Substances chimiques
Bacterial Proteins
0
Fimbriae Proteins
147680-16-8
Transcription Factors
0
CtrA protein, Caulobacter
0
DNA-Binding Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
703Subventions
Organisme : NIH HHS
ID : 1R15GM139127
Pays : United States
Organisme : NIH HHS
ID : 1R15GM139127
Pays : United States
Organisme : NIH HHS
ID : 1R15GM139127
Pays : United States
Informations de copyright
© 2024. The Author(s).
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