Regulation of carbon metabolic fluxes to enhance lipid and succinate production in oleaginous fungus Mortierella alpina.
Mortierella alpina
Carbon conversion
Carbon flux
Lipid
Succinic acid
Journal
World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472
Informations de publication
Date de publication:
12 Aug 2024
12 Aug 2024
Historique:
received:
14
01
2024
accepted:
14
07
2024
medline:
12
8
2024
pubmed:
12
8
2024
entrez:
11
8
2024
Statut:
epublish
Résumé
Mortierella alpina is popular for lipid production, but the low carbon conversion rate and lipid yield are major obstacles for its economic performance. Here, external addition of organic acids involved in tricarboxylic acid cycle was used to tune carbon flux and improve lipid production. Citrate was determined to be the best organic acid that can be used for enhancing lipid production. By the addition of citrate, the lipid titer and content were approximately 1.24 and 1.34 times higher, respectively. Meanwhile, citrate supplement also promoted the accumulation of succinate, an important value-added platform chemical. Owing to the improved lipid and succinate production through adding citrate, the carbon conversion rate of M. alpina reached up to 52.17%, much higher than that of the control group (14.11%). The addition of citrate could redistribute carbon flux by regulating the expression level of genes related to tricarboxylic acid cycle metabolism. More carbon fluxes flow to lipid and succinate synthesis, which greatly improved the carbon conversion efficiency of M. alpina. This study provides an effective and straightforward strategy with potential economic benefits to improve carbon conversion efficiency in M. alpina.
Identifiants
pubmed: 39128979
doi: 10.1007/s11274-024-04082-z
pii: 10.1007/s11274-024-04082-z
doi:
Substances chimiques
Succinic Acid
AB6MNQ6J6L
Carbon
7440-44-0
Citric Acid
2968PHW8QP
Lipids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
298Subventions
Organisme : The Start-up Funding of Central South University
ID : Grant No. 202045023
Organisme : The Natural Science Foundation of Hunan Province
ID : Grant No. 2021JJ30803
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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