Chromosome-scale pearl millet genomes reveal CLAMT1b as key determinant of strigolactone pattern and Striga susceptibility.

Striga / genetics Lactones / metabolism Pennisetum / genetics Genome, Plant Plant Proteins / genetics Chromosomes, Plant / genetics Plant Diseases / parasitology Methyltransferases / metabolism Plant Weeds / genetics Disease Resistance / genetics Plant Growth Regulators / metabolism

Journal

Nature communications

ISSN: 2041-1723

Titre abrégé: Nat Commun

Pays: England

ID NLM: 101528555

Informations de publication

Date de publication:
12 Aug 2024

Historique:

received: 11 02 2024

accepted: 01 08 2024

medline: 13 8 2024

pubmed: 13 8 2024

entrez: 12 8 2024

Statut: epublish

Résumé

The yield of pearl millet, a resilient cereal crop crucial for African food security, is severely impacted by the root parasitic weed Striga hermonthica, which requires host-released hormones, called strigolactones (SLs), for seed germination. Herein, we identify four SLs present in the Striga-susceptible line SOSAT-C88-P10 (P10) but absent in the resistant 29Aw (Aw). We generate chromosome-scale genome assemblies, including four gapless chromosomes for each line. The Striga-resistant Aw lacks a 0.7 Mb genome segment containing two putative CARLACTONOIC ACID METHYLTRANSFERASE1 (CLAMT1) genes, which may contribute to SL biosynthesis. Functional assays show that P10CLAMT1b produces the SL-biosynthesis intermediate methyl carlactonoate (MeCLA) and that MeCLA is the precursor of P10-specific SLs. Screening a diverse pearl millet panel confirms the pivotal role of the CLAMT1 section for SL diversity and Striga susceptibility. Our results reveal a reason for Striga susceptibility in pearl millet and pave the way for generating resistant lines through marker-assisted breeding or direct genetic modification.

Identifiants

DOI: 10.1038/s41467-024-51189-w PMID: 39134551

pubmed: 39134551

doi: 10.1038/s41467-024-51189-w

pii: 10.1038/s41467-024-51189-w

doi:

Substances chimiques

Lactones 0

Plant Proteins 0

Methyltransferases EC 2.1.1.-

Plant Growth Regulators 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

6906

Subventions

Organisme : Bill and Melinda Gates Foundation (Bill & Melinda Gates Foundation)

ID : OPP1136424

Organisme : King Abdullah University of Science and Technology (KAUST)

ID : baseline

Informations de copyright

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