Single-cell transcriptomics reveal transcriptional programs underlying male and female cell fate during Plasmodium falciparum gametocytogenesis.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
26 Aug 2024
26 Aug 2024
Historique:
received:
02
06
2023
accepted:
01
08
2024
medline:
27
8
2024
pubmed:
27
8
2024
entrez:
26
8
2024
Statut:
epublish
Résumé
The Plasmodium falciparum life cycle includes obligate transition between a human and mosquito host. Gametocytes are responsible for transmission from the human to the mosquito vector where gamete fusion followed by meiosis occurs. To elucidate how male and female gametocytes differentiate in the absence of sex chromosomes, we perform FACS-based cell enrichment of a P. falciparum gametocyte reporter line followed by single-cell RNA-seq. In our analyses we define the transcriptional programs and predict candidate driver genes underlying male and female development, including genes from the ApiAP2 family of transcription factors. A motif-driven, gene regulatory network analysis indicates that AP2-G5 specifically modulates male development. Additionally, genes linked to the inner membrane complex, involved in morphological changes, are uniquely expressed in the female lineage. The transcriptional programs of male and female development detailed herein allow for further exploration of the evolution of sex in eukaryotes and provide targets for future development of transmission blocking therapies.
Identifiants
pubmed: 39187486
doi: 10.1038/s41467-024-51201-3
pii: 10.1038/s41467-024-51201-3
doi:
Substances chimiques
Protozoan Proteins
0
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7177Subventions
Organisme : Vetenskapsrådet (Swedish Research Council)
ID : 2021-05057
Organisme : Vetenskapsrådet (Swedish Research Council)
ID : 2021-06602
Organisme : Vetenskapsrådet (Swedish Research Council)
ID : SNIC 2020/16-146
Organisme : Vetenskapsrådet (Swedish Research Council)
ID : SNIC 2021/22-492
Organisme : Vetenskapsrådet (Swedish Research Council)
ID : VR 2021-06602
Organisme : Svenska Sällskapet för Medicinsk Forskning (Swedish Society for Medical Research)
ID : Stora Anslaget
Informations de copyright
© 2024. The Author(s).
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