Synthetic biology toolkit of Ralstonia eutropha (Cupriavidus necator).

Cupriavidus necator / genetics Synthetic Biology / methods Metabolic Engineering / methods Polyhydroxyalkanoates / metabolism Biofuels
Ralstonia eutropha (Cupriavidus necator) Bioproduction Industrial biotechnology Metabolic engineering Standardized parts Synthetic biology toolkit

Journal

Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612

Informations de publication

Date de publication:
29 Aug 2024
Historique:
received: 06 06 2024
accepted: 12 08 2024
revised: 09 08 2024
medline: 31 8 2024
pubmed: 31 8 2024
entrez: 29 8 2024
Statut: epublish

Résumé

Synthetic biology encompasses many kinds of ideas and techniques with the common theme of creating something novel. The industrially relevant microorganism, Ralstonia eutropha (also known as Cupriavidus necator), has long been a subject of metabolic engineering efforts to either enhance a product it naturally makes (polyhydroxyalkanoate) or produce novel bioproducts (e.g., biofuels and other small molecule compounds). Given the metabolic versatility of R. eutropha and the existence of multiple molecular genetic tools and techniques for the organism, development of a synthetic biology toolkit is underway. This toolkit will allow for novel, user-friendly design that can impart new capabilities to R. eutropha strains to be used for novel application. This article reviews the different synthetic biology techniques currently available for modifying and enhancing bioproduction in R. eutropha. KEY POINTS: • R. eutropha (C. necator) is a versatile organism that has been examined for many applications. • Synthetic biology is being used to design more powerful strains for bioproduction. • A diverse synthetic biology toolkit is being developed to enhance R. eutropha's capabilities.

Identifiants

DOI: 10.1007/s00253-024-13284-2 PMID: 39207499
pubmed: 39207499
doi: 10.1007/s00253-024-13284-2
pii: 10.1007/s00253-024-13284-2
doi:

Substances chimiques

Polyhydroxyalkanoates 0
Biofuels 0

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

450

Subventions

Organisme : Bundesministerium für Bildung und Forschung
ID : 031B1339A

Informations de copyright

© 2024. The Author(s).

Références

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Auteurs

Lara Santolin (L)

Technische Universität Berlin, Institute of Biotechnology, Chair of Bioprocess Engineering, Berlin, Germany.

Sebastian L Riedel (SL)

Berliner Hochschule Für Technik, Department VIII - Mechanical Engineering, Event Technology and Process Engineering, Environmental and Bioprocess Engineering Laboratory, Berlin, Germany. Sebastian.Riedel@bht-berlin.de.
ORCID: 0000-0003-1373-417X

Christopher J Brigham (CJ)

Department of Bioengineering, University of Massachusetts Dartmouth, North Dartmouth, MA, USA. cbrigham@umassd.edu.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus necator › Synthetic biology toolkit of Ralstonia...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Biologie synthétique › Synthetic biology toolkit of Ralstonia...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Génie métabolique › Synthetic biology toolkit of Ralstonia...
questionsmedicales.fr › Lipides › Polyhydroxyalcanoates › Synthetic biology toolkit of Ralstonia...
questionsmedicales.fr › Environnement et santé publique › Environnement › Ressources de production d'énergie › Énergie renouvelable › Biocarburants › Synthetic biology toolkit of Ralstonia...