Assessment of the Safety and Exopolysaccharide Synthesis Capabilities of Bacillus amyloliquefaciens D189 Based on Complete Genome and Phenotype Analysis.
Journal
Current microbiology
ISSN: 1432-0991
Titre abrégé: Curr Microbiol
Pays: United States
ID NLM: 7808448
Informations de publication
Date de publication:
03 Sep 2024
03 Sep 2024
Historique:
received:
22
02
2024
accepted:
20
06
2024
medline:
3
9
2024
pubmed:
3
9
2024
entrez:
3
9
2024
Statut:
epublish
Résumé
Exopolysaccharides (EPS) are natural macromolecular carbohydrates with good functional activity and physiological activities, which can be utilized as an emulsifier, viscosity enhancer, stabilizer, gelling agent, and water retention agent in a wide range of food products. In this study, the whole genome of Bacillus amyloliquefaciens D189, an EPS-producing bacteria, was sequenced. The result showed that D189 contains a single, circular chromosome of 3,963,356 bp with an average GC content of 45.74% and 3996 coding genes. The gene annotation results showed that D189 is a potentially safe strain and confirmed to be safe associated with hemolytic assay, and antibiotic resistance test. Meanwhile, D189 genome possessed 240 genes related to carbohydrate metabolism. More importantly, D189 could transport 9 sugars and contained a complete biosynthetic pathway for 8 nucleotide sugars. Based on the validation experiments, strain D189 could metabolize 8 sugars (glucose, sucrose, trehalose, fructose, cellobiose, maltose, mannitol, and N-acetyl-D-glucosamine) to produce EPS, with the highest yield of 1.212 g/L when sucrose was the carbon source. Therefore, the whole genome sequencing preliminarily elucidated the physiological mechanism of EPS, providing several pathways for engineering D189 to further enhance the yield of EPS.
Identifiants
pubmed: 39225770
doi: 10.1007/s00284-024-03777-8
pii: 10.1007/s00284-024-03777-8
doi:
Substances chimiques
Polysaccharides, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
342Subventions
Organisme : National Natural Science Foundation of China
ID : 32160558
Organisme : Key projects in Guangxi
ID : 2019GXNSFDA245008
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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