DUF1127-containing protein and ProQ had opposite effects on biofilm formation in Vibrio alginolyticus.
Vibrio alginolyticus
Biofilm
DUF1127
ProQ
RNA-binding protein
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
07 Sep 2024
07 Sep 2024
Historique:
received:
11
06
2024
accepted:
02
09
2024
medline:
8
9
2024
pubmed:
8
9
2024
entrez:
7
9
2024
Statut:
epublish
Résumé
The RNA binding protein is crucial for gene regulation at the post transcription level. In this study, functions of the DUF1127-containing protein and ProQ, which are RNA-binding proteins, were revealed in Vibrio alginolyticus. DUF1127 deletion increased the ability of biofilm formation, whereas ProQ deletion reduced the amount of biofilm. Moreover, extracellular proteinase secretion was significantly reduced in the DUF1127 deletion strain. ProQ, not DUF1127-containing protein, can help the cell to defense oxidative stress. Deletion of DUF1127 resulted in a higher ROS level in the cell, however, ProQ deletion showed no difference. RNA-seq unveiled the expression of genes involved in extracellular protease secretion were significantly downregulated and biofilm synthesis-related genes, such as rbsB and alsS, were differentially expressed in the DUF1127 deletion strain. ProQ affected the expression of genes involved in biofilm synthesis (flgC and flgE), virulence (betB and hutG), and oxidative stress. Moreover, the DUF1127-containing and ProQ affected the mRNA levels of various regulators, such as LysR and BetI. Overall, our study revealed that the DUF1127-containing protein and ProQ have crucial functions on biofilm formation in V. alginolyticus.
Identifiants
pubmed: 39244528
doi: 10.1186/s12866-024-03486-z
pii: 10.1186/s12866-024-03486-z
doi:
Substances chimiques
Bacterial Proteins
0
RNA-Binding Proteins
0
Reactive Oxygen Species
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
330Subventions
Organisme : National Natural Science Foundation of China
ID : 42276158
Organisme : Guang dong Natural Science Foundation-General Project
ID : 2024A1515010759
Organisme : Initial Funding of Jiangsu University of Technology
ID : KYY24053
Informations de copyright
© 2024. The Author(s).
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