Functionalization of microbubbles in a microfluidic chip for biosensing application.
Biosensor
Microbubble
Reusable
Surface functionalization
Journal
Biomedical microdevices
ISSN: 1572-8781
Titre abrégé: Biomed Microdevices
Pays: United States
ID NLM: 100887374
Informations de publication
Date de publication:
17 Sep 2024
17 Sep 2024
Historique:
accepted:
27
08
2024
medline:
17
9
2024
pubmed:
17
9
2024
entrez:
17
9
2024
Statut:
epublish
Résumé
Microbubbles are widely used for biomedical applications, ranging from imagery to therapy. In these applications, microbubbles can be functionalized to allow targeted drug delivery or imaging of the human body. However, functionalization of the microbubbles is quite difficult, due to the unstable nature of the gas/liquid interface. In this paper, we describe a simple protocol for rapid functionalization of microbubbles and show how to use them inside a microfluidic chip to develop a novel type of biosensor. The microbubbles are functionalized with biochemical ligand directly at their generation inside the microfluidic chip using a DSPE-PEG-Biotin phospholipid. The microbubbles are then organized inside a chamber before injecting the fluid with the bioanalyte of interest through the static bubbles network. In this proof-of-concept demonstration, we use streptavidin as the bioanalyte of interest. Both functionalization and capture are assessed using fluorescent microscopy thanks to fluorescent labeled chemicals. The main advantages of the proposed technique compared to classical ligand based biosensor using solid surface is its ability to rapidly regenerate the functionalized surface, with the complete functionalization/capture/measurement cycle taking less than 10 min.
Identifiants
pubmed: 39287824
doi: 10.1007/s10544-024-00721-2
pii: 10.1007/s10544-024-00721-2
doi:
Substances chimiques
Streptavidin
9013-20-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
39Subventions
Organisme : EIPHI Graduate school
ID : ANR-17-EURE-0002
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Références
C.-L. Azzopardi, V. Lacour, J.-F. Manceau, M. Barthès, D. Bonnet, F. Chollet, T. Leblois, A fluidic interface with high flow uniformity for reusable large area resonant biosensors. Micromachines 8(10), 308 (2017). https://doi.org/10.3390/mi8100308
doi: 10.3390/mi8100308
C.-L. Azzopardi, F. Chollet, J.-F. Manceau, W. Boireau, Analyte capture in an array of functionalized droplets for a regenerable biosensor. Biomicrofluidics 13(5), 054105 (2019). https://doi.org/10.1063/1.5115494
doi: 10.1063/1.5115494
E. Beguin, L. Bau, S. Shrivastava, E. Stride, Comparing strategies for magnetic functionalization of microbubbles. ACS Applied Materials & Interfaces 11(2), 1829–1840 (2019). https://doi.org/10.1021/acsami.8b18418
doi: 10.1021/acsami.8b18418
S.H. Cho, J.H. Jeong, S.R. Yang, B.Y. Kim, J.-D. Kim, Binding evaluation of targeted microbubbles with biotin-avidin interaction by surface plasmon resonance biosensor. Jpn. J. Appl. Phys. 45(1S), 421 (2006). https://doi.org/10.1143/jjap.45.421
doi: 10.1143/jjap.45.421
S.M. Chowdhury, L. Abou-Elkacem, T. Lee, J. Dahl, A.M. Lutz, Ultrasound and microbubble mediated therapeutic delivery: underlying mechanisms and future outlook. J. Control. Release 326, 75–90 (2020). https://doi.org/10.1016/j.jconrel.2020.06.008
P.B. Duncan, D. Needham, Test of the epstein- plesset model for gas microparticle dissolution in aqueous media: effect of surface tension and gas undersaturation in solution. Langmuir 20(7), 2567–2578 (2004). https://doi.org/10.1021/la034930i
doi: 10.1021/la034930i
C.M. Dundas, D. Demonte, S. Park, Streptavidin-biotin technology: improvements and innovations in chemical and biological applications. Appl. Microbiol. Biotechnol. 97, 9343–9353 (2013). https://doi.org/10.1007/s00253-013-5232-z
doi: 10.1007/s00253-013-5232-z
M. Focsan, A. Campu, A.-M. Craciun, M. Potara, C. Leordean, D. Maniu, S. Astilean, A simple and efficient design to improve the detection of biotin-streptavidin interaction with plasmonic nanobiosensors. Biosens. Bioelectron. 86, 728–735 (2016). https://doi.org/10.1016/j.bios.2016.07.054
doi: 10.1016/j.bios.2016.07.054
E. Fuguet, C. Ràfols, M. Rosés, E. Bosch, Critical micelle concentration of surfactants in aqueous buffered and unbuffered systems. Anal. Chim. Acta 548(1–2), 95–100 (2005). https://doi.org/10.1016/j.aca.2005.05.069
doi: 10.1016/j.aca.2005.05.069
R. Gramiak, P.M. Shah, Echocardiography of the aortic root. Invest. Radiol. 3(5), 356–366 (1968). https://doi.org/10.1097/00004424-196809000-00011
doi: 10.1097/00004424-196809000-00011
P. Kogan, R.C. Gessner, P.A. Dayton, Microbubbles in imaging: applications beyond ultrasound. Bubble Sci. Eng. Technol. 2(1), 3–8 (2010). https://doi.org/10.1179/175889610X12730566149100
doi: 10.1179/175889610X12730566149100
V. Lacour, C. Elie-Caille, T. Leblois, J.J. Dubowski, Regeneration of a thiolated and antibody functionalized gaas (001) surface using wet chemical processes. Biointerphases 11(1), (2016). https://doi.org/10.1116/1.4942878
S.A.G. Langeveld, B. Meijlink, K. Kooiman, Phospholipid-coated targeted microbubbles for ultrasound molecular imaging and therapy. Curr. Opin. Chem. Biol. 63, 171–179 (2021). https://doi.org/10.1016/j.cbpa.2021.04.013
doi: 10.1016/j.cbpa.2021.04.013
T. Muller, BiAcoustic PID Flowrate. GitLab (2022). https://gitlab.com/mllr.tristan/projetm2
A. Oseev, T. Lecompte, F. Remy-Martin, G. Mourey, F. Chollet, B.L.R. Boiseaumarie, A. Rouleau, O. Bourgeois, E. Maistre, C. Elie-Caille, J.-F. Manceau, W. Boireau, T. Leblois, Assessment of shear-dependent kinetics of primary haemostasis with a microfluidic acoustic biosensor. IEEE Trans. Biomed. Eng. 68(8), 2329–2338 (2021). https://doi.org/10.1109/tbme.2020.3031542
doi: 10.1109/tbme.2020.3031542
A.D. Petelska, M. Naumowicz, Z.A. Figaszewski, The influence of ph on phosphatidylethanolamine monolayer at the air/aqueous solution interface. Cell Biochem. Biophys. 65(2), 229–235 (2012). https://doi.org/10.1007/s12013-012-9424-4
L. Pinon, L. Montel, O. Mesdjian, M. Bernard, A. Michel, C. Ménager, J. Fattaccioli, Kinetically enhanced fabrication of homogeneous biomimetic and functional emulsion droplets. Langmuir 34(50), 15319–15326 (2018). https://doi.org/10.1021/acs.langmuir.8b02721
doi: 10.1021/acs.langmuir.8b02721
T.M. Squires, R.J. Messinger, S.R. Manalis, Making it stick: convection, reaction and diffusion in surface-based biosensors. Nat. Biotechnol. 26(4), 417–426 (2008). https://doi.org/10.1038/nbt1388
doi: 10.1038/nbt1388
M. Sypabekova, A. Hagemann, D. Rho, S. Kim, Review: 3-aminopropyltriethoxysilane (aptes) deposition methods on oxide surfaces in solution and vapor phases for biosensing applications. Biosensors 13(1), 36 (2022). https://doi.org/10.3390/bios13010036
doi: 10.3390/bios13010036
A.M. Takalkar, A.L. Klibanov, J.J. Rychak, J.R. Lindner, K. Ley, Binding and detachment dynamics of microbubbles targeted to p-selectin under controlled shear flow. J. Control. Release 96(3), 473–482 (2004). https://doi.org/10.1016/j.jconrel.2004.03.002
N. Tarchichi, F. Chollet, J.-F. Manceau, New regime of droplet generation in a T-shape microfluidic junction. Microfluid. Nanofluid. 14, 45–51 (2013). https://doi.org/10.1007/s10404-012-1021-8
doi: 10.1007/s10404-012-1021-8
E.C. Unger, T.O. Matsunaga, T. McCreery, P. Schumann, R. Sweitzer, R. Quigley, Therapeutic applications of microbubbles. European J. Radiol. 160–168 (2002). https://doi.org/10.1016/s0720-048x(01)00455-7
E. Unger, T. Porter, J. Lindner, P. Grayburn, Cardiovascular drug delivery with ultrasound and microbubbles. Adv. Drug Deliv. Rev. 72, 110–126 (2014). https://doi.org/10.1016/j.addr.2014.01.012
doi: 10.1016/j.addr.2014.01.012
M. Versluis, E. Stride, G. Lajoinie, B. Dollet, T. Segers, Ultrasound contrast agent modeling: A review. Ultrasound Med. Biol. 46(9), 2117–2144 (2020). https://doi.org/10.1016/j.ultrasmedbio.2020.04.014
S. Wang, J.A. Hossack, A.L. Klibanov, Targeting of microbubbles: contrast agents for ultrasound molecular imaging. J. Drug Target. 26(5–6), 420–434 (2018). https://doi.org/10.1080/1061186x.2017.1419362
J.M. Warram, A.G. Sorace, R. Saini, H.R. Umphrey, K.R. Zinn, K. Hoyt, A triple-targeted ultrasound contrast agent provides improved localization to tumor vasculature. J. Ultrasound Med. 30(7), 921–931 (2011). https://doi.org/10.7863/jum.2011.30.7.921
doi: 10.7863/jum.2011.30.7.921
J. Yang, X. Miao, Y. Guan, C. Chen, S. Chen, X. Zhang, X. Xiao, Z. Zhang, Z. Xia, T. Yin, Z. Hei, W. Yao, Microbubble functionalization with platelet membrane enables targeting and early detection of sepsis-induced acute kidney injury. Adv. Healthcare Mater. 10(23), 2101628 (2021). https://doi.org/10.1002/adhm.202101628
doi: 10.1002/adhm.202101628
L. Ye, R. Pelton, M.A. Brook, Biotinylation of tio2 nanoparticles and their conjugation with streptavidin. Langmuir 23(10), 5630–5637 (2007). https://doi.org/10.1021/la0626656
doi: 10.1021/la0626656
J.S.-M. Yeh, C.A. Sennoga, E. McConnell, R. Eckersley, M.-X. Tang, S. Nourshargh, J.M. Seddon, D.O. Haskard, P. Nihoyannopoulos, A targeting microbubble for ultrasound molecular imaging. PLoS ONE 10(7), 0129681 (2015). https://doi.org/10.1371/journal.pone.0129681
doi: 10.1371/journal.pone.0129681