Regulatory mechanism of C4-dicarboxylates in cyclo (Phe-Pro) production.
Burkholderia seminalis
Anti-fungus
C4-dicarboxylates
Cyclo (Phe-Pro)
Dct system
Journal
Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812
Informations de publication
Date de publication:
28 Sep 2024
28 Sep 2024
Historique:
received:
08
07
2024
accepted:
11
09
2024
medline:
29
9
2024
pubmed:
29
9
2024
entrez:
28
9
2024
Statut:
epublish
Résumé
Cyclo (Phe-Pro) (cFP), a cyclic dipeptide with notable antifungal, antibacterial, and antiviral properties, shows great promise for biological control of plant diseases. Produced as a byproduct by non-ribosomal peptide synthetases (NRPS), the regulatory mechanism of cFP biosynthesis remains unclear. In a screening test of 997 Tn5 mutants of Burkholderia seminalis strain R456, we identified eight mutants with enhanced antagonistic effects against Fusarium graminearum (Fg). Among these, mutant 88's culture filtrate contained cFP, confirmed through HPLC and LC-MS, which actively inhibited Fg. The gene disrupted in mutant 88 is part of the Dct transport system (Dct-A, -B, -D), responsible for C4-dicarboxylate transport. Knockout mutants of Dct genes exhibited higher cFP levels than the wild type, whereas complementary strains showed no significant difference. Additionally, the presence of exogenous C4-dicarboxylates reduced cFP production in wild type R456, indicating that these substrates negatively regulate cFP synthesis. Given that cFP synthesis is related to NRPS, we previously identified an NRPS cluster in R456, horizontally transferred from algae. Specifically, knocking out gene 2061 within this NRPS cluster significantly reduced cFP production. A Fur box binding site was predicted upstream of gene 2061, and yeast one-hybrid assays confirmed Fur protein binding, which increased with additional C4-dicarboxylates. Knockout of the Fur gene led to up-regulation of gene 2061 and increased cFP production, suggesting that C4-dicarboxylates suppress cFP synthesis by enhancing Fur-mediated repression of gene 2061.
Identifiants
pubmed: 39342283
doi: 10.1186/s12934-024-02527-6
pii: 10.1186/s12934-024-02527-6
doi:
Substances chimiques
Peptides, Cyclic
0
Peptide Synthases
EC 6.3.2.-
Dicarboxylic Acids
0
non-ribosomal peptide synthase
EC 6.3.2.-
Bacterial Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
255Subventions
Organisme : National Natural Science Foundation of China
ID : 31872017, 32072472
Organisme : The Zhejiang Provincial Natural Science Foundation of China
ID : LZ24C140004
Organisme : Key Research and Development Project in Zhejiang Province
ID : 2019C02035
Organisme : Hangzhou Science and Technology Development Plan Project
ID : 20231203A05
Organisme : State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products
ID : 2010DS700124-KF202205
Informations de copyright
© 2024. The Author(s).
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