A snapshot of the Physcomitrella N-terminome reveals N-terminal methylation of organellar proteins.
AARE
N-terminus
Organelle
Proteases
Protein methylation
Protein modification
Protein targeting
Journal
Plant cell reports
ISSN: 1432-203X
Titre abrégé: Plant Cell Rep
Pays: Germany
ID NLM: 9880970
Informations de publication
Date de publication:
03 Oct 2024
03 Oct 2024
Historique:
received:
13
06
2024
accepted:
13
09
2024
medline:
3
10
2024
pubmed:
3
10
2024
entrez:
3
10
2024
Statut:
epublish
Résumé
Analysis of the N-terminome of Physcomitrella reveals N-terminal monomethylation of nuclear-encoded, mitochondria-localized proteins. Post- or co-translational N-terminal modifications of proteins influence their half-life as well as mediating protein sorting to organelles via cleavable N-terminal sequences that are recognized by the respective translocation machinery. Here, we provide an overview on the current modification state of the N-termini of over 4500 proteins from the model moss Physcomitrella (Physcomitrium patens) using a compilation of 24 N-terminomics datasets. Our data reveal distinct proteoforms and modification states and confirm predicted targeting peptide cleavage sites of 1,144 proteins localized to plastids and the thylakoid lumen, to mitochondria, and to the secretory pathway. In addition, we uncover extended N-terminal methylation of mitochondrial proteins. Moreover, we identified PpNTM1 (P. patens alpha N-terminal protein methyltransferase 1) as a candidate for protein methylation in plastids, mitochondria, and the cytosol. These data can now be used to optimize computational targeting predictors, for customized protein fusions and their targeted localization in biotechnology, and offer novel insights into potential dual targeting of proteins.
Identifiants
pubmed: 39361041
doi: 10.1007/s00299-024-03329-1
pii: 10.1007/s00299-024-03329-1
doi:
Substances chimiques
Plant Proteins
0
Mitochondrial Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
250Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : EXC-2189
Organisme : Deutsche Forschungsgemeinschaft
ID : IG9/8
Informations de copyright
© 2024. The Author(s).
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