Active-bromide and surfactant synergy for enhanced microfouling control.
Biofilms
/ drug effects
Surface-Active Agents
/ pharmacology
Pseudomonas aeruginosa
/ drug effects
Anti-Bacterial Agents
/ pharmacology
Bromides
/ pharmacology
Staphylococcus aureus
/ drug effects
Biofouling
/ prevention & control
Drug Synergism
Hydrophobic and Hydrophilic Interactions
Disinfectants
/ pharmacology
Seawater
/ microbiology
Octoxynol
/ pharmacology
Active-bromide
Antibacterial activity
Biofilm
Biofouling control
Condenser cooling system
Surfactant dispersal
Journal
Archives of microbiology
ISSN: 1432-072X
Titre abrégé: Arch Microbiol
Pays: Germany
ID NLM: 0410427
Informations de publication
Date de publication:
10 Oct 2024
10 Oct 2024
Historique:
received:
31
05
2024
accepted:
29
09
2024
revised:
16
09
2024
medline:
11
10
2024
pubmed:
11
10
2024
entrez:
10
10
2024
Statut:
epublish
Résumé
Biofilms are structured microbial communities encased in a matrix of self-produced extracellular polymeric substance (EPS) and pose significant challenges in various industrial cooling systems. A nuclear power plant uses a biocide active-bromide for control of biological growth in its condenser cooling system. This study is aimed at evaluating the anti-bacterial and anti-biofilm efficacy of active-bromide against planktonic and biofilm-forming bacteria that are commonly encountered in seawater cooling systems. The results demonstrated that active-bromide at the concentration used at the power plant (1 ppm) exhibited minimal killing activity against Pseudomonas aeruginosa planktonic cells. The bacterial cell surface hydrophobicity assay using Staphylococcus aureus and P. aeruginosa indicated that Triton-X 100 significantly decreased the hydrophobicity of planktonic cells, enhancing the susceptibility of the cells to active-bromide. Biofilm inhibition assays revealed limited efficacy of active-bromide at 1 ppm concentration, but significant inhibition at 5 ppm and 10 ppm. However, the addition of a surfactant, Triton-X 100, in combination with 1 ppm active-bromide displayed a synergistic effect, leading to significant biofilm dispersal of pre-formed P. aeruginosa biofilms. This observation was substantiated by epifluorescence microscopy using a live/dead bacterial assay that showed the combination treatment resulted in extensive cell death within the biofilm, as indicated by a marked increase in red fluorescence, compared to treatments with either agent alone. These findings suggest that active bromide alone may be insufficient for microfouling control in the seawater-based condenser cooling system of the power plant. Including a biocompatible surfactant that disrupts established biofilms (microfouling) can significantly improve the efficacy of active bromide treatment.
Identifiants
pubmed: 39387929
doi: 10.1007/s00203-024-04154-6
pii: 10.1007/s00203-024-04154-6
doi:
Substances chimiques
Surface-Active Agents
0
Anti-Bacterial Agents
0
Bromides
0
Disinfectants
0
Octoxynol
9002-93-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
430Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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