GhMAC3e is involved in plant growth and defense response to Verticillium dahliae.
Gossypium
/ genetics
Gene Expression Regulation, Plant
Plant Diseases
/ microbiology
Plant Proteins
/ genetics
Disease Resistance
/ genetics
Plants, Genetically Modified
Arabidopsis
/ genetics
Ascomycota
/ physiology
Indoleacetic Acids
/ metabolism
Plant Growth Regulators
/ metabolism
Gene Silencing
Stress, Physiological
/ genetics
Verticillium
Verticillium dahliae
Auxin
Cotton
GhMAC3e
MOS4-associated complex
Journal
Plant cell reports
ISSN: 1432-203X
Titre abrégé: Plant Cell Rep
Pays: Germany
ID NLM: 9880970
Informations de publication
Date de publication:
10 Oct 2024
10 Oct 2024
Historique:
received:
13
08
2024
accepted:
30
09
2024
medline:
11
10
2024
pubmed:
11
10
2024
entrez:
10
10
2024
Statut:
epublish
Résumé
GhMAC3e expression was induced by various stresses and hormones. GhMAC3e may regulate plant growth by influencing auxin distribution, and play important roles in Verticillium wilt resistance via mediating SA signaling. The MOS4-Associated Complex (MAC) is a highly conserved protein complex involved in pre-mRNA splicing and spliceosome assembly, which plays a vital role in plant immunity. It comprises key components such as MOS4, CDC5, and PRL1. MAC3A/B, as U-box E3 ubiquitin ligases, are crucial for various plant processes including development, stress responses, and disease resistance. However, their roles in cotton remain largely unknown. In this study, we first cloned the GhMAC3e gene from cotton and explored its biological functions by using virus-induced gene silencing (VIGS) in cotton and transgenic overexpression in Arabidopsis. The results showed that GhMAC3e is ubiquitously expressed in cotton tissues and could be induced by salt stress, Verticillium dahliae (VD) infection, PEG, ABA, ETH, GA3, MeJA, and SA. Silencing GhMAC3e retarded primary stem growth and reduced biomass of cotton coupled with the reduced auxin content in the petioles and veins. Silencing GhMAC3e up-regulated expression of cell growth-related genes GhXTH16 and Gh3.6, while down-regulated GhSAUR12 expression. Ectopic expression of GhMAC3e in Arabidopsis significantly enhanced its resistance to Verticillium wilt (VW) in terms of decreased pathogen biomass and lowered plant mortality. Overexpression of GhMAC3e dramatically upregulated AtPR1 by around 15 fold and more than 262 fold under basal and VD inoculation condition, respectively. This change was not associated with the expression of GhNPR1. In conclusion, GhMAC3e may not only regulate plant growth by influencing auxin distribution and growth-related gene expression, but also play important roles in VW resistance via mediating SA signaling independent of NPR1 transcription level.
Identifiants
pubmed: 39390296
doi: 10.1007/s00299-024-03348-y
pii: 10.1007/s00299-024-03348-y
doi:
Substances chimiques
Plant Proteins
0
Indoleacetic Acids
0
Plant Growth Regulators
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
259Subventions
Organisme : National Natural Science Foundation of China
ID : No. 31371672
Organisme : National Natural Science Foundation of China
ID : No. 32172083
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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