The MTR4/hnRNPK complex surveils aberrant polyadenylated RNAs with multiple exons.
Humans
Exons
/ genetics
Heterogeneous-Nuclear Ribonucleoprotein K
/ metabolism
RNA, Messenger
/ genetics
RNA-Binding Proteins
/ metabolism
HeLa Cells
HEK293 Cells
Exosome Multienzyme Ribonuclease Complex
/ metabolism
Polyadenylation
Nucleocytoplasmic Transport Proteins
/ genetics
Introns
/ genetics
RNA Nucleotidyltransferases
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
17 Oct 2024
17 Oct 2024
Historique:
received:
11
09
2023
accepted:
21
08
2024
medline:
18
10
2024
pubmed:
18
10
2024
entrez:
17
10
2024
Statut:
epublish
Résumé
RNA surveillance systems degrade aberrant RNAs that result from defective transcriptional termination, splicing, and polyadenylation. Defective RNAs in the nucleus are recognized by RNA-binding proteins and MTR4, and are degraded by the RNA exosome complex. Here, we detect aberrant RNAs in MTR4-depleted cells using long-read direct RNA sequencing and 3' sequencing. MTR4 destabilizes intronic polyadenylated transcripts generated by transcriptional read-through over one or more exons, termed 3' eXtended Transcripts (3XTs). MTR4 also associates with hnRNPK, which recognizes 3XTs with multiple exons. Moreover, the aberrant protein translated from KCTD13 3XT is a target of the hnRNPK-MTR4-RNA exosome pathway and forms aberrant condensates, which we name KCTD13 3eXtended Transcript-derived protein (KeXT) bodies. Our results suggest that RNA surveillance in human cells inhibits the formation of condensates of a defective polyadenylated transcript-derived protein.
Identifiants
pubmed: 39419981
doi: 10.1038/s41467-024-51981-8
pii: 10.1038/s41467-024-51981-8
doi:
Substances chimiques
Heterogeneous-Nuclear Ribonucleoprotein K
0
HNRNPK protein, human
146410-60-8
RNA, Messenger
0
RNA-Binding Proteins
0
TENT4B protein, human
EC 2.7.7.-
Exosome Multienzyme Ribonuclease Complex
EC 3.1.-
Nucleocytoplasmic Transport Proteins
0
RNA Nucleotidyltransferases
EC 2.7.7.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8684Subventions
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 17KK0163
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 21H04792
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 23H04955
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 21H00243
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 21H02758
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 21K19402
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 22KK0285
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 22K15093
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 22H04925
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : 20H00624
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : JP21ae0121049
Informations de copyright
© 2024. The Author(s).
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