Molecular-based characterization and bioengineering of Sorghum bicolor to enhance iron deficiency tolerance in iron-limiting calcareous soils.
Sorghum
/ genetics
Soil
/ chemistry
Iron Deficiencies
Gene Expression Regulation, Plant
Iron
/ metabolism
Plant Proteins
/ genetics
Oryza
/ genetics
Plants, Genetically Modified
Alkyl and Aryl Transferases
/ genetics
Hordeum
/ genetics
Azetidinecarboxylic Acid
/ analogs & derivatives
Bioengineering
Siderophores
/ metabolism
Promoter Regions, Genetic
Bioengineering
Calcareous soil
Energy plant
Iron deficiency tolerance
Rice
Sorghum bicolor
Journal
Plant molecular biology
ISSN: 1573-5028
Titre abrégé: Plant Mol Biol
Pays: Netherlands
ID NLM: 9106343
Informations de publication
Date de publication:
24 Oct 2024
24 Oct 2024
Historique:
received:
01
10
2023
accepted:
16
09
2024
medline:
25
10
2024
pubmed:
25
10
2024
entrez:
24
10
2024
Statut:
epublish
Résumé
Plant biomass can significantly contribute to alternative energy sources. Sorghum bicolor is a promising plant for producing energy, but is susceptible to iron deficiency, which inhibits its cultivation in iron-limiting calcareous soils. The molecular basis for the susceptibility of sorghum to iron deficiency remains unclear. Here, we explored the sorghum genome to identify genes involved in iron uptake and translocation. Iron deficiency-responsive gene expression was comparable to that in other graminaceous plants. A nicotianamine synthase gene, SbNAS1, was induced in response to iron deficiency, and SbNAS1 showed enzyme activity. Sorghum secreted 2'-deoxymugineic acid and other phytosiderophores under iron deficiency, but their levels were relatively low. Intercropping of sorghum with barley or rice rescued iron deficiency symptoms of sorghum. To produce bioengineered sorghum with enhanced tolerance to iron deficiency, we introduced four cassettes into sorghum: 35S promoter-OsIRO2 for activation of iron acquisition-related gene expression, SbIRT1 promoter-Refre1/372 for enhanced ferric-chelate reductase activity, and barley IDS3, and HvNAS1 genomic fragments for enhanced production of phytosiderophores and nicotianamine. The resultant single sorghum line exhibited enhanced secretion of phytosiderophores, increased ferric-chelate reductase activity, and improved iron uptake and leaf greenness compared with non-transformants under iron-limiting conditions. Similar traits were also conferred to rice by introducing the four cassettes. Moreover, these rice lines showed similar or better tolerance in calcareous soils and increased grain iron accumulation compared with previous rice lines carrying two or three comparable cassettes. These results provide a molecular basis for the bioengineering of sorghum tolerant of low iron availability in calcareous soils.
Identifiants
pubmed: 39448407
doi: 10.1007/s11103-024-01508-y
pii: 10.1007/s11103-024-01508-y
doi:
Substances chimiques
Soil
0
Iron
E1UOL152H7
Plant Proteins
0
nicotianamine synthase
EC 2.5.-
Alkyl and Aryl Transferases
EC 2.5.-
Azetidinecarboxylic Acid
5GZ3E0L9ZU
2'-deoxymugineic acid
74235-24-8
nicotianamine
34441-14-0
Siderophores
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
117Subventions
Organisme : Japan Society for the Promotion of Science
ID : JP15K18658
Organisme : Japan Society for the Promotion of Science
ID : JP15KK0286
Organisme : Japan Society for the Promotion of Science
ID : JP20K05777
Organisme : Japan Society for the Promotion of Science
ID : JP18H02115
Organisme : Japan Society for the Promotion of Science
ID : JP22H02231
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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